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We narrowed to 725 results for: ACE

Showing: 301 - 320 of 725 results

  1. Common Injection Routes in Mice

    Type
    Blog Post
    ...but it actually requires more technical skill to place the needle in the correct vein without collapsing...injections are given in the peritoneal cavity, or the space surrounding organs in the abdomen. They are a popular...cerebral spinal fluid. The injection site is the space in and around the vertebrae, so hitting the correct...

  2. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...Korte et al., 2021) for all disposable items and surfaces that come into contact with the rAAV and apply...centrifugation (e.g., iodixanol) using a high-speed ultracentrifuge. During the spin, the virions will form a band...ddPCRAAV Purification by Iodixanol Gradient UltracentrifugationAAV Production in HEK293 Cells...

  3. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ...technology that allows precise modifications of DNA (replacements, small insertions, and deletions) without introducing.... The pegRNA consists of a target-complementary spacer, a scaffold sequence, and an extension sequence..., Raguram, A., & Liu, D. R. (2019). Search-and-replace genome editing without double-strand breaks or ...

  4. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    ...proteins (Wrapp et al., 2020), which sit on the surface of coronaviruses and enable the virus to enter ...in the molecular crevices proteins on the cell surface. Figure 1: Comparison of common antibody,... is tricky because they have fewer hydrophilic surfaces, require detergents or lipids to remain folded...

  5. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    ...definitive, but visual identification is a good place to start before taking on more time consuming and...fitness cost yet. However, many fluorescent proteins face similar limitations, and many labs are already using...Figure 4). They created ShadowR by modifying the surface of the chromoprotein Ultramarine with hydrophilic...

  6. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...formation of disulfide bridges, glycosylation and acetylation to create functional, stable proteins. Protein...range of proteins including antibodies and pharmaceuticals, specifically interleukins. Yeast expression...biochemistry. When using S. cerevisiae, researchers often place recombinant proteins under the control of the galactose...

  7. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    ...the genome and located immediately 5’ to a protospacer adjacent motif (PAM) sequence. Co-delivery of wild-type...produces the phenotype you screened for in the first place. With the proper experimental design and validation...

  8. Plasmids 101: Protein tags

    Type
    Blog Post
    ... who need to overexpress and purify proteins can face any number of technical challenges depending on ...protease. A TEV protease cleavage site is frequently placed between two tags being used for two rounds of purification...purification, with the cleavage reaction taking place between column runs. The TEV protease itself, with...

  9. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...on their earlier Voltron indicators (a fusion of Ace2N and HaloTag, Figure 4), they determined that an ...-to-D mutation in the related voltage indicator Ace2N-mNeon also led to similar improvements in performance...www.janelia.org/open-science/voltron-and-positron.  Both Ace2N-mNeon-A122D and Voltron2 are available as Cre-dependent...

  10. How to Write a Scientific Review Article

    Type
    Blog Post
    ... Thomas D. Gilmore. "Histone acetyltransferases and histone deacetylases in B-and T-cell development, ...GenBank, but it was useful to present it all in one place to get a sense of the similarities and differences...

  11. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ... and excision recombination reactions that take place when lambda phage infects bacteria. In vivo, these...reactions described below: The BP Reaction takes place between the attB sites flanking the insert and the...gene expression.     The LR Reaction takes place between the attL sites of the generated entry clone...

  12. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...science. Facebook is continuing its growth, and while many publishers and researchers have Facebook pages...article, find blogs about the study, or even review Facebook and Twitter comments. On We Share science, video...

  13. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    ...conference can be intimidating. And it can be exacerbated when everyone seems to know one another, but ...program to Microbe 2020 in Chicago.  Finding our place in networking and mentoring circles We looked at...this first year. Mentors Amanda Gunn, Bryan Gibb, Candace Williams, Dave Baltrus, Geof Hannigan, Irene Newton...

  14. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...overexpressing mice spent more time in the risky bright place, driven by their motivation to explore novel environments...treatment in rodents. In this test, an animal is placed into a tank of water with no support from below...attempts to escape, or passive floating on the water surface. The latter reflects a state of behavioral despair...

  16. Lab to Office Culture Shock

    Type
    Blog Post
    ...frequently why many people get into science in the first place. The feeling of camaraderie one gets from a well-functioning...could dread these interactions, but you could also embrace them. Talking about science to non-scientists can...curiosity that led you into science in the first place. Finally, if you still find the idea of not holding...

  17. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    ...focused on therapeutic strategies that use gene replacement therapy, gene editing, MECP2 reactivation, RNA...RNA editing, RNA trans-splicing, and protein replacement to reverse the impacts of this disease.  As a...is a strong translational candidate for gene replacement therapy. In 2014, RSRT brought together a strong...

  18. Hot Plasmids - August 2020

    Type
    Blog Post
    ...in the cytosol (instead of on the yeast extracellular surface) which enables tracking of protease activity...fluorescent proteins might not localize to the right places, which can provide misleading information on where...

  19. The Challenges of Cell Culture

    Type
    Blog Post
    ...forensic technique (aka DNA fingerprinting) that can place suspects at the scene of a crime (every human in...experience through working for a variety of pharmaceutical companies including GSK, AstraZeneca-MedImmune...talks and seminars on cell culture and other pharmaceutical topics. The LGC Group also provides standards...

  20. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...protein attaches to cell surface receptors, other important binding events take place during the course of...viral envelop and the receptors on the target cell surface. The possible interactions and hence possible targets...
Showing: 301 - 320 of 725 results