...indicated by exclamation marks. Error points include places where DNA is being created, such as PCR amplification...request the lab provide a new, sequence-verified replacement for the plasmid, and the full QC process is started... started over again after receiving the replacement plasmid at Addgene. QC is a team effort Addgene’s ...

...formation of disulfide bridges, glycosylation and acetylation to create functional, stable proteins. Protein...range of proteins including antibodies and pharmaceuticals, specifically interleukins. Yeast expression...biochemistry. When using S. cerevisiae, researchers often place recombinant proteins under the control of the galactose...

...proteins (Wrapp et al., 2020), which sit on the surface of coronaviruses and enable the virus to enter ...in the molecular crevices proteins on the cell surface. Figure 1: Comparison of common antibody,... is tricky because they have fewer hydrophilic surfaces, require detergents or lipids to remain folded...

...definitive, but visual identification is a good place to start before taking on more time consuming and...fitness cost yet. However, many fluorescent proteins face similar limitations, and many labs are already using...Figure 4). They created ShadowR by modifying the surface of the chromoprotein Ultramarine with hydrophilic...

...but it actually requires more technical skill to place the needle in the correct vein without collapsing...injections are given in the peritoneal cavity, or the space surrounding organs in the abdomen. They are a popular...cerebral spinal fluid. The injection site is the space in and around the vertebrae, so hitting the correct...

...Korte et al., 2021) for all disposable items and surfaces that come into contact with the rAAV and apply...centrifugation (e.g., iodixanol) using a high-speed ultracentrifuge. During the spin, the virions will form a band...ddPCRAAV Purification by Iodixanol Gradient UltracentrifugationAAV Production in HEK293 Cells...

...technology that allows precise modifications of DNA (replacements, small insertions, and deletions) without introducing.... The pegRNA consists of a target-complementary spacer, a scaffold sequence, and an extension sequence..., Raguram, A., & Liu, D. R. (2019). Search-and-replace genome editing without double-strand breaks or ...

...the genome and located immediately 5’ to a protospacer adjacent motif (PAM) sequence. Co-delivery of wild-type...produces the phenotype you screened for in the first place. With the proper experimental design and validation...

... who need to overexpress and purify proteins can face any number of technical challenges depending on ...protease. A TEV protease cleavage site is frequently placed between two tags being used for two rounds of purification...purification, with the cleavage reaction taking place between column runs. The TEV protease itself, with...

...in the cytosol (instead of on the yeast extracellular surface) which enables tracking of protease activity...fluorescent proteins might not localize to the right places, which can provide misleading information on where...

...conference can be intimidating. And it can be exacerbated when everyone seems to know one another, but ...program to Microbe 2020 in Chicago.  Finding our place in networking and mentoring circles We looked at...this first year. Mentors Amanda Gunn, Bryan Gibb, Candace Williams, Dave Baltrus, Geof Hannigan, Irene Newton...

... Thomas D. Gilmore. "Histone acetyltransferases and histone deacetylases in B-and T-cell development, ...GenBank, but it was useful to present it all in one place to get a sense of the similarities and differences...

... and excision recombination reactions that take place when lambda phage infects bacteria. In vivo, these...reactions described below: The BP Reaction takes place between the attB sites flanking the insert and the...gene expression.     The LR Reaction takes place between the attL sites of the generated entry clone...

...science. Facebook is continuing its growth, and while many publishers and researchers have Facebook pages...article, find blogs about the study, or even review Facebook and Twitter comments. On We Share science, video...

...recently moved to our brand new location, with more space to grow and help scientists share resources in new...segmentation during embryogenesis. Typically, regularly spaced denticles (bristles) outline the segments of the...actually clump together forming one patch on the surface, giving the embryo a “spiny and prickly look” that...

...frequently why many people get into science in the first place. The feeling of camaraderie one gets from a well-functioning...could dread these interactions, but you could also embrace them. Talking about science to non-scientists can...curiosity that led you into science in the first place. Finally, if you still find the idea of not holding...

...on their earlier Voltron indicators (a fusion of Ace2N and HaloTag, Figure 4), they determined that an ...-to-D mutation in the related voltage indicator Ace2N-mNeon also led to similar improvements in performance...www.janelia.org/open-science/voltron-and-positron.  Both Ace2N-mNeon-A122D and Voltron2 are available as Cre-dependent...

...focused on therapeutic strategies that use gene replacement therapy, gene editing, MECP2 reactivation, RNA...RNA editing, RNA trans-splicing, and protein replacement to reverse the impacts of this disease.  As a...is a strong translational candidate for gene replacement therapy. In 2014, RSRT brought together a strong...

...overexpressing mice spent more time in the risky bright place, driven by their motivation to explore novel environments...treatment in rodents. In this test, an animal is placed into a tank of water with no support from below...attempts to escape, or passive floating on the water surface. The latter reflects a state of behavioral despair...

...forensic technique (aka DNA fingerprinting) that can place suspects at the scene of a crime (every human in...experience through working for a variety of pharmaceutical companies including GSK, AstraZeneca-MedImmune...talks and seminars on cell culture and other pharmaceutical topics. The LGC Group also provides standards...