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We narrowed to 490 results for: ARA-2

Showing: 301 - 320 of 490 results

  1. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ...would label everything but the CD8+ T cells (Figure 2). Even better, we can use the same fluorophore for...gating on the negative population.     Figure 2: Dump gating labels all unwanted cell lineages with...conjugated to a biotin tag but not a fluorophore. In a separate step, cells are incubated with a fluorophore-conjugated...the intranuclear ones. You do not need to use a separate fix/perm cytosolic procedure in that case.   ...cell population. To detect non-surface markers, a separate intracellular labeling protocol is necessary, ...

  2. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...to a specific area at the plasma membrane (Figure 2). Just express the plasma membrane-localized iLID ...cells will be ready for the spotlight.    Figure 2: Opto-RhoGEFs to control Rho GTPase activity. A) Schematic...cytoskeleton. They provide cells with physical structure, separate chromosomes during mitosis, support transport ... called a katana. Opto-katanin consists of two separate blue light-sensitive systems, VVDfast to control...your protein of interest and express the other separately, then use blue light to generate CRY2-CIB1 oligomers...

  3. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    ...melanogaster." Molecular and cellular endocrinology 215.1-2 (2004): 1-10. PubMed PMID: 15026169. Soo, Rochelle...algae host for reproduction. Snail parasite Leucochloridium is a parasitic worm that infects a snail’s eyestalks...Brusca and Gilligan, 1983). Grasshopper parasite The parasitic worm Spinochordodes tellinii‘s larvae makes...ve also carved pumpkins (yes, Blugene made an appearance there too) and shared fascinatingly creepy science...Tongue-eating louse Cymothoa exigua is a marine parasite that enters fish through its gills. It attaches...replacement in a marine fish (Lutjanus guttatus) by a parasitic isopod (Crustacea: Isopoda)." Copeia 1983.3 (1983...

  4. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    ...environmental contaminants.  Figure 2: An Addgenie works in the tissue culture hood while wearing...doi.org/10.1290/1071-2690(2002)038<0086:caeomi>2.0.co;2 Uphoff CC, Drexler HG (2011) Detecting Mycoplasma ...of the causes of contamination. If possible, a separate lab should be made available for cell culturing...keeping them in the pockets of your lab coats. Have separate footwear for the lab to avoid bringing in environmental...wide range of mycoplasma. These methods cannot guarantee complete removal of mycoplasma but, can get rid...made permanent damage to the cell culture it is irreparable. Some cells may die due to longer incubation ...Microbiol .44: 42–49  Uphoff Cc, Drexler Hg (2002) Comparative Antibiotic Eradication of Mycoplasma Infections...

  5. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ...wheat germ, HeLa, and Leishmania (Gagoski, et al 2015[2]). The proteins encoded in this set range in size ...to generate Illumina sequencing libraries using a 2-step PCR-based protocol and are compatible with single-end...centered within GFP/RFP bicistronic reporters characterized in Cambray et al., 2013. These terminators can...size from 4 to 156 kDa, enabling the user to characterise the efficiency of their cell-free system in correlation...

  6. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    ...tagging." Nature methods (2015). PubMed PMID:26322837. 2. Stadler, Charlotte, et al. "Immunofluorescence and...the fusions had some negative effect on these parameters. IT5 and IT6 displayed good binding efficiency...

  7. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    ...Agrobacterium infection. At approximately 7 kb, pSiM24 is 2 and 6 kb smaller than commercially available pCAMBIA...duplicated enhancer domains and characterized in studies of tobacco, Arabidopsis, and corn. For both β-glucuronidase...plant infection, as well as plasmid yield during preparation in E. coli. Addgene depositor Indu Maiti has ...functions in multiple species, including tobacco and Arabidopsis. Improving Agrobacterium binary vectors Vectors...efficiently inherited in both transgenic tobacco and Arabidopsis. Finally, Sahoo et al. demonstrate that this ...

  8. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    ...elution choices: TE, Tris buffer, or water Figure 2: Tris-EDTA, Tris, or water? The buffer you choose ..., destabilizing the molecule and favoring the separation of the two helixes. Low pH instead results in... buffer for preserving the stability of your preparation for a long time. Tris buffer controls the pH,... if the priority is to directly use your DNA preparation in any downstream application involving enzymatic...buffer is that it allows better control of the pH preparation stabilizing the plasmid, and still allowing most...at -20°C or even at -80°C, in this case your preparation can be eluted in water or in your buffer of preference...

  10. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ...sequence is unique compared to the rest of the genome.  2) The target is present immediately upstream of a Protospacer...utilized allowing for PAM flexibility. Fig. 2: Prime editor engaging target DNA. The reverse transcriptase...Walton et al., 2020). Further, this group also characterized a second Cas9 variant, SpRY, which cuts at NRN...

  11. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ...faster when using a dual guide system.   Fig. 2: Size screening for large deletion events.   ... Biol 17: https://doi.org/10.1186/s13059-016-1012-2 Find more CRISPR Resources at Addgene: Read our CRISPRGuide...visualized by running your PCR on a gel. It’s still paramount that you sequence clones to verify that the deletions...

  12. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    ...Almo, Institute for Protein Innovation  1:18:45 - 2:01:18 (end) - Panel Alex Tucker (Ginkgo Bioworks)...their curation of citable, linkable figures. Cause #2: Hidden “negative” data We all want to be able to ... and here I summarize what I learned both in preparation for my talk and at the minisymposium. You can...address this issue directly by creating well characterised antibodies against all human extracellular proteins...stories and read papers wherein a researcher tries 5 separate experiments at once to get confirmatory data for...

  13. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

    Type
    Blog Post
    ...Transgenesis." PLoS ONE. 8(12): e83043 (20 December 2013). 2. Carola Engler et al. "A One Pot, One Step, Precision...Lohmann said they’ve only tested GreenGate in Arabidopsis, it should in principle work in any plant species...

  14. Let There Be LITE Plasmids

    Type
    Blog Post
    ...First, the light-responsive molecules of cryptochrome 2 are the chromophores flavin-adenine dinucleotide and...ingredients - CRY2 and CIB1 – which they borrowed from Arabidopsis thaliana. The results are light-inducible transcriptional...

  15. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...six different research constellations: Doench/Root (2), Zhang (3), Wu (4), Yusa (5), Wang/Lander/Sabatini...Bioinformatics 33.7 (2016): 1099-1100. PubMed PMID: 28414855. 2. Doench, John G., et al. "Optimized sgRNA design to...single cell level), are separated by, for example, cell sorting. The separated cells are subsequently ...which gRNAs are differentially expressed in the separate groups and, as a consequence, which genes are ...the protein following stimulation would then be separated by sorting, and subsequently sequenced to identify...suggested gRNA sequence including adapters. Output_UserInputParams: Contains all the information that you ...al. "Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach." Nature methods...

  16. Finding Your Science Policy Path

    Type
    Blog Post
    ...teaching and mentoring others (see more in Figure 2). Starting early demonstrates not only your interest...application process so you can learn more! Figure 2: The many ways to prepare for a science policy career...the work you do to creatively connect seemingly disparate individuals and ideas toward solving one problem...evidence-based university policy to combat sexual harassment in academia or a large company who decides how...

  17. Antibodies 101: Validation

    Type
    Blog Post
    ...another. You’ll need to validate that points 1 and 2 hold true under your specific experimental conditions...when (1) other strategies may not be feasible and (2) large-scale proteomic and transcriptomic datasets...antigen - Recognition of an immunogen does not guarantee recognition of the target antigen. You want to... transfect it into your favorite cell line. In parallel, you transfect cells with another plasmid not ...

  18. RUBY-Red Siliques

    Type
    Blog Post
    ...make it easier to study these mutants.   Fig. 2: (A) Betacyanin is produced from tyrosine by the activity...occurs in T0 plants (from callus) or T1 plants (Arabidopsis floral dip). In the next generation the CRISPR...planning to use it to simplify my work with an Arabidopsis mutant that can’t set seeds. I study the development... Fig. 3: (Top right) A RUBY-expressing Arabidopsis seed following floral dip transformation next...without antibiotics. (Top left) Mature silique (Arabidopsis fruit) with fertilized developing seeds from ... the Plant Genome Using CRISPR/Cas9 Tips for Arabidopsis Transformation CRISPR 101: CRISPR-Mediated Plant...

  19. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

    Type
    Blog Post
    ... stabilization of unknown bacteria. Figure 2: Christina Mork collecting samples at the hot spring...microfluidic parallelization to sequence this highly complex hot spring sample. The sample was separated into ...Quake Lab member joined for half of the trip. Preparations ahead of time included contacting YNP staff ... Paul and Anastasia (Potanina) Neddersen characterized a few samples by 16S rRNA gene sequencing which...thermostable Cas9 enzymes have previously been characterized: GeoCas9 from the Doudna lab and ThermoCas9 ...

  20. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...phosphoinositides (Varnai and Balla, 2008) (Figure 2). RNA production can be detected by using a MS2 coat... 296.5569 (2002): 913-916. PubMed PMID: 11988576. 2. Tsien, Roger Y. "The green fluorescent protein." ...microscopy (University of Amsterdam). He develops, characterizes, and uses genetically encoded fluorescent probes...
Showing: 301 - 320 of 490 results