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  1. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ... glycosylation patterns. Surface labeling is particularly useful for classifying and sorting cells by ...Paul Heisig is a Research Associate in the lab of Arlene Sharpe at Harvard Medical School. His projects ...

  2. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...into the parental plasmid (or other). This is particularly relevant in cases where a large plasmid with...introduce a point mutation in a large construct. Particularly, if a pair of unique restriction site is located...

  3. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ...using the same expression plasmid. This is a particularly important control when creating a phosphoserine-incorporation...Institute for Research in Madison, Wisconsin. She is particularly interested in the intersection of cell signaling...

  4. Evolution of Lab Techniques

    Type
    Blog Post
    ...cars become more technologically advanced leaving earlier versions obsolete, the techniques we use in lab...researchers at a time when sequencing was still in the early days of development. Today, researchers use both...

  5. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ...this way, if you know what you need. Most often, early career scientists aren’t trained to have these types...based on financial values. All too often, we see early investigators making unnecessary short term sacrifices...

  6. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...requires a nearby PAM, although these are still early days for this technology, and the user may need ... P-Y, Daley G, Church G (2013) Optimization of scarless human stem cell genome editing. Nucleic Acids ...

  7. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...different system (Issa et al., 2023). This can be particularly burdensome in the brain where many different...such as chicken-beta-actin or CAG can drive strong early gene expression, but can become methylated over ...

  8. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...unanticipated sites. While off-target effects are nearly impossible to eliminate, it’s essential to understand...appropriate method for an activator or repressor Cas. Similarly, Cas ChIP-Seq would also identify all DNA binding...

  9. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...specific changes into the mouse genome. With these early technologies, a researcher would first introduce... is a molecular biologist with Biogen and is particularly interested in applying CRISPR for mouse genome...

  10. Antibodies 101: Validation

    Type
    Blog Post
    ... does not mean that is all it will bind to - particularly when you start using complex samples such as... the antibody webpages. These outlets can be particularly useful for sharing negative data. Negative data...

  11. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...waivers. One of the blog posts that helped me quite early on was Christine Liu’s “How to Become a Scientist...on the Addgene blog Check out our resources for early career researchers Get advice for applying for jobs...

  12. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ...to recognize DNA sequences is not ideal. For the early generation gene editors, new amino acid recognition...engineering of novel base editor proteins. She is particularly interested in combining the realms of art and...

  13. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ... different colors. This lends flow cytometry particularly well to complex experiments looking at a variety...from the use of secondary antibodies. It’s also particularly useful for intracellular staining where binding...

  14. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...bacteria or cell-free systems. The approach is particularly useful for producing drug precursors, biofuels...works in both mammalian systems and Drosophila. Similarly, the Multi-Kingdom (MK) Golden Gate system was...

  15. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...Initial photoactivatable CRISPR systems published in early 2015 focused on using light to control transcription...Moritoshi Sato’s lab at the University of Tokyo and Charles Gersbach’s lab at Duke University developed similar...

  16. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...Early CRISPR applications were often limited by the low editing efficiency of homology-directed repair...nickase (Nishida et al., 2016). Target-AID acts similarly but not identically to BE3, modifying a 3-5 base...

  17. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...enough to get good separation in your ladder, particularly in the sizes around your protein of interest... leftover sample) to fill the relevant wells. Similarly, if you load a gel and then forget to start it...

  18. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...provides improvements to classical cloning methods, particularly for complex transgenesis constructs. Kirchmaier...terminator). Next, up to six Level 1 modules can be similarly assembled into a Level 2 vector, thus forming ...

  19. Antibodies 101 Series on TikTok

    Type
    Blog Post
    ... Addgene We'll be posting these animations regularly from now until May, so follow us to get updates...

  20. The Twelve Days of Antibodies

    Type
    Blog Post
    ...Antibody, Addgene gave to me…  A recombinant Anti-Arl13b!    🎶On the fifth day of Antibody, Addgene gave...
Showing: 301 - 320 of 576 results