Addgene

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An Inside Look into Addgene's Dev Team

Type

Blog Post

...in iterative chunks, we have the flexibility to adapt and easily respond to changing needs of the teams...
Neuronal labeling with Spaghetti Monster

Type

Blog Post

...smFPs in the future. References 1. Viswanathan, Sarada, et al. "High-performance probes for light and ...
Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

Type

Blog Post

...Genetic compensation triggered by mutant mRNA degradation." Nature 568.7751 (2019): 193. PubMed PMID: 30944477...
Fluorescent Proteins 101: Aptamer Fluorophores

Type

Blog Post

...nucleotide-based ligand binders would prove to be highly adaptable tools. The key to aptamers’ flexibility is their...
Mesothelioma - Causes, Symptoms, and Treatment

Type

Blog Post

...like the Asbestos Disease Awareness Organization (ADAO) are working toward such a ban, and the president...
Antibiotic Resistance: An Old Solution but a New Problem

Type

Blog Post

...bacteriology and drug resistance. It seems as though, nowadays, we cannot make it a full month without a new,...
Using Video to Share Your Science: We Share Science

Type

Blog Post

...published in scientific journals flies under the radar and gains little notice. A 2009 research study found...
CRISPR Activation: A Practical Guide

Type

Blog Post

...doi.org/10.1038/nmeth.3871 Gilbert LA, Horlbeck MA, Adamson B, Villalta JE, Chen Y, Whitehead EH, Guimaraes...
Adeno-associated Viruses (AAVs) for Genome Editing

Type

Blog Post

...and pAAV-TK-Acceptor) so that they can be easily adapted to edit your gene of choice. These vectors are ...
Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

Type

Blog Post

...that a fusion protein is cleaved by proteases. Nowadays, with ample structural information available, ...
Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

Type

Blog Post

... visualization of two colors. Similarly, Flybow adapts Brainbow 2.0 for use in Drosophila melanogaster...
Plasmids 101: Biotinylation

Type

Blog Post

.... The biotin-avidin interaction has also been adapted for use in immunohistochemistry and antibody labelling...
Quick Guide to Near-Infrared Fluorescent Proteins

Type

Blog Post

... changes occurring due to protein synthesis, degradation, or reconstitution (reviewed in Shcherbakova ...
Five Popular Model Organisms, Part 2

Type

Blog Post

...plant species and metabolic properties affecting adaptations. Arabidopsis thaliana flowers. Image from...
Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

Type

Blog Post

...solution is to include an in-frame degron, or degradation sequence, upstream of the 5’ splice donor and...
Antibodies 101: Normalization and Loading Controls for Western Blots

Type

Blog Post

...the gel (n=3). Data is presented as mean ± SEM. Adapted from Analytical Biochemistry, vol 440, Gilda, J...
Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

Type

Blog Post

...parts. Gibson assembly meets CRISPR Gibson can be adapted to more complicated cloning schemes, such as those...
Treating Muscular Dystrophy with CRISPR Gene Editing

Type

Blog Post

...low-frequency indels will not cause frameshifts. Figure adapted from Osterout et al. (2015b) under a CC-BY 4.0 ...
Technologies Enabled by NanoLuc® Luciferase

Type

Blog Post

...accuators. The researchers took a FRET reporter and adapted it into a BRET reporter by using NanoLuc® Luciferase...
Viral Vectors 101: Optogenetic Tools

Type

Blog Post

...10.1016/j.sbi.2019.05.006 Vierock, J., Rodriguez-Rozada, S., Dieter, A., Pieper, F., Sims, R., Tenedini...

Showing: 301 - 320 of 337 results

Results per page:

An Inside Look into Addgene's Dev Team

Neuronal labeling with Spaghetti Monster

Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

Fluorescent Proteins 101: Aptamer Fluorophores

Mesothelioma - Causes, Symptoms, and Treatment

Antibiotic Resistance: An Old Solution but a New Problem

Using Video to Share Your Science: We Share Science

CRISPR Activation: A Practical Guide

Adeno-associated Viruses (AAVs) for Genome Editing

Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

Plasmids 101: Biotinylation

Quick Guide to Near-Infrared Fluorescent Proteins

Five Popular Model Organisms, Part 2

Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

Antibodies 101: Normalization and Loading Controls for Western Blots

Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

Treating Muscular Dystrophy with CRISPR Gene Editing

Technologies Enabled by NanoLuc® Luciferase

Viral Vectors 101: Optogenetic Tools

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