...Isopropanol or Lithium Chloride. This protocol was adapted from (Link opens in a new window) Chomczynski P...is not as stable as DNA and is susceptible to degradation by heat, RNases, and other enzymes. Pro-Tips ...reagents cold and work quickly to reduce RNA degradation. Keep work area, equipment, and reagents RNase-free...Having your samples spin at 4°C helps reduce RNA degradation. If you don’t have access to a refrigerated centrifuge... your RNA sample(s) at -80 °C to prevent RNA degradation and avoid multiple freeze-thaw cycles. Pro-Tip...

...distinguishable thanks to the use of phenol red. Image adapted from Zolotukhin, S., et al. "Recombinant adeno-...indicates the location of the purified AAV. Left image adapted from Zolotukhin, S., et al. "Recombinant adeno-... the purified AAV using option #1. Right image adapted by permission from Macmillan Publishers Ltd: Nature...proteins VP1, VP2, VP3; M protein marker. Image adapted from Strobel Benjamin, Miller Felix D., Rist Wolfgang...

...selection with carbenicillin; however it can be adapted for any antibiotic through the use of a selection...

...protocol was developed for 293T cells but can be adapted to a variety of target cell lines and selection...

...HEK293T cell line should work. This approach can be adapted for different cell lines and different transfection...

...protocol was developed for A549 cells but can be adapted to a variety of target cell lines and selection...

...protocol was established using 293T cells but can be adapted to alternative cell lines. Workflow Timeline Day...

...protocol uses a dry transfer device but can be adapted for wet and semi-dry transfer approaches. Last ...

...adequate quality, but this upfront cost will save headaches and expense later. At a minimum we recommend the...

...Institute's TRC shRNA Design Process . To minimize degradation of off-target mRNAs, use NCBI’s BLAST program...