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We narrowed to 710 results for: BASE;

Showing: 301 - 320 of 710 results

  1. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...immunoglobulin genes, recombinant antibodies are plasmid-based. Their sequences can be optimized for specificity...Arbabi-Ghahroudi et al., 2017). Although they’re based on the immune system of llamas, synthetic nanobodies...mixture of proteins. The proteins are separated based on molecular weight by SDS-PAGE, and are subsequently...well as tools for developing and producing plasmid-based antibodies - and we’re always growing our collection...

  2. Using a Light Microscope Protocol

    Type
    Protocol
    ...microscope with labels. Created with BioRender.com. Base Light Source Condenser and Diaphragm Stage Objective...carry it with two hands - one hand supporting the base and the other holding the arm - or use a cart. Revolve...

  3. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...lab at Duke University developed similar systems based on the light-inducible heterodimerizing cryptochrome...systems have been developed for use with CRISPR-based gene editing (see table below). For example, the...possibilities for optogenetic manipulation of CRISPR-based systems in animal models. Use Anti-CRISPRs to Turn...Hasegawa S, Yoshimoto K, Sato M (2017) CRISPR–Cas9-based photoactivatable transcription systems to induce..., Kawano F, Suzuki H, Sato M (2015) CRISPR-Cas9-based Photoactivatable Transcription System. Chemistry...transcription Zhou et al., 2020   NPOM-caged nucleobases Cas9, photocaged-gRNA Gene editing For more...

  4. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ... Santalucía Lab has developed a cloning toolkit based on the Multisite Gateway® technology from Life Technologies...MultiSite Gateway Kit is a customizable Gateway®-based toolkit that allows scientists to generate fusion... Medical School has recently developed a CRISPR-based multicolor tool enabling the imaging of multiple...cancer-associated chromosome shattering. This method is based on the recognition of genomic loci by three orthogonal...does it work? RUSH is an ingenious two-state assay based on the reversible interaction between a hook protein...Neveu laboratory designed a new iterative, chaining-based cloning method that simplifies the process of constructing...presented Archaerhodopsin 3 (Arch) as a new rhodopsin based voltage indicator for use in the detection of neuronal...

  7. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    ... 2015). All of Addgene’s antibodies are plasmid-based and recombinant. Learn more about recombinant antibodies...affinity reagents, such as nanobodies and ScFvs, based on community demand. Figure 1: Antibody...from Addgene via our Antibody Data Hub once the database is available. This data will help the collection...

  8. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    ... time, we’re developing ourselves as a knowledge base for the processes of viral production and testing...connections. Expanding the viral vector knowledge base: You can help! We’ve been expanding our viral vector...

  9. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    ...alternative careers. Science Careers has developed a web-based, career-planning platform called the Individual ...to learn about a variety of science professions based on their skills and interests. Once scientists identify...

  10. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...found in my tissue.” It is based on the same principles as other antibody-based assays, like an ELISA or...cases, a whole organism. But, as with any antibody-based technique, validation and proper experimental design...different companies, such as Validated Antibody Database and CiteAb. The antibody you choose can make or...

  11. Retrovirus Plasmids

    Type
    Collection
    ...pCI-VSVG Envelope VSV-G recommended for the FELIX (FIV-based) system Nolan 35617 pCAG-Eco Envelope Ecotropic ...plasmid with your gene of interest? Search our database for retroviral transfer plasmids that have inserts...

  12. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    ...should be in real scientific practice, so it is based on a win-win strategy. After each team presented...developed to teach gamma-retrovirus or lentivirus-based ex-vivo gene therapy’ strategies to undergraduates...

  13. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    ...interaction approaches that of GST-glutathione. Biotin-based immobilization for kinetic analysis Proteolytic ...bacteria. Our initial attempt to create a plasmid based on the biotin ligase BirA backbone (Eric Campeau...

  14. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    ...discriminating between neurons for light microscopy-based neuronal reconstruction. To achieve stochastic multicolor...conventional transgenic strategies. In such vector-based gene delivery systems, the stochastic expression...

  16. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    ... remotely can be more difficult if your work is based in the lab. If you’re unsure what to do during this...submitting a registered report, where peer review is based on the methods, not the results. Also consider using...

  17. Viral Vectors 101: Transductions

    Type
    Blog Post
    ...long-term (AAV). If you perform a lipid-based or electrical-based transfection, most of the nucleic acid...

  18. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ...Topoisomerase based cloning (TOPO cloning) is a DNA cloning method that does not use restriction enzymes...polymerase has an error rate of about 1 in 3,500 bases. Typically polymerases with proofreading functionality... relies on the basic ability of complementary basepairs adenine (A) and thymine (T) to hybridize and form...

  20. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ... MACS. But MACS usually only allows for sorting based on a single (lineage) marker, while fluorescence-activated...biorender.com.   Dye stains Dye stains are non-antibody-based stains that bind DNA or free amines of proteins....cytosolic and/or nuclear markers. Lastly, stains, based not on antibodies but on small molecule dyes, are...
Showing: 301 - 320 of 710 results