We narrowed to 799 results for: Clu

... cascades (reviewed in Abreu & Levitz, 2020), including light-sensitive chimeric GPCRS (OptoXRs) (Tichy...experiments!  First, closely read the publication, including the supplemental data. You’re most likely to find...hardware, optogenetics tools, viruses, and more.  Conclusion   Optogenetic tools provide a powerful way to...

... variants that are less sensitive to ethanol, including the “middle” ADH1 promoter pADH1m, which maintains...Liqun Luo’s lab developed the Q system from a gene cluster in the fungus Neurospora crassa. The QUAS promoter...3 in a single modular vector for ease of use! Conclusion This post contains only a brief overview of some...

...fluorescence-based approach. These challenges can include background autofluorescence from the plates or ...researchers to track endogenous activity of EGFR, including dose-dependent response to EGF (Promega, 2025)...led to subtle changes in co-receptor dynamics, including NanoBRET assays to monitor the binding of fluorescently-labeled...

...mentioned above. The initial IMP cohort of 78 mentees included: 47 women (60.2%) 20 graduate students (25.6%...directly attributable to participation in the program included improvements in writing fellowships, giving oral...impressions of the IMP experience. Conference highlights included remarks by the Spanish Secretary of Research, ...

...Wild-type A549 cells do not express CAs9 and are included as a control (WT). Vinculin is a loading control... assay before, consider following their tips, including: Before digesting the DNA duplexes with Surveyor...PAGE instead of agarose to get sharper bands. Conclusion Overall, making a human cell line with a gene...

...phenotype in order to conclude that the phenotype is due to an on-target effect. Conclusions Selection of gRNAs...modification of interest, it would be unwise to make conclusions on the basis of the activity of a single gRNA...

...this time, it is! Factorial ANOVAs include two-way ANOVAs but can include many more than two independent ... Of course, a one-tailed test also works for exclusively less-than comparisons. Paired and unpaired t-tests...

...reproducibility of experiments and the validity of conclusions. Antibody engineering also benefits antibody-...antibody. (B) Examples of engineered antibodies, including chimerization, conjugation, and antibody fragments...There are different ways to achieve this goal, including error-prone PCR, degenerate primers, gene shuffling...

...applications for dsDNA inserts include: large insertions or deletions, including selectable DNA fragments, ...Promoters commonly used to control expression of Red include the IPTG-inducible lac promoter, the arabinose-...

...Science is about fighting scientific partisanship, including more scientists in our leadership, and raising... Anschutz Medical Campus.  He studies viruses including HIV and is interested in global health policy....Committee about climate change. All the scientists – including global warming skeptics – insisted that more data...

...on immune system Conventional cancer therapies include radiation therapy (owing to contributions from ...blocking. Other molecular targets under research include TIM-3, LAG-3, STAT-3, EGFR, CD20 and CD73 on specific... factors researchers are beginning to focus on include: Cell heterogeneity Tumors are composed of a wide...

..., 1998), this 15 amino acid peptide contains a cluster of four cysteines (CCXXCC, where X is any non-cysteine...with minimal cross-reactivity. Other strategies include using engineered ligases to attach a ligand to ...study your gene of interest with these tools. Conclusion Researchers and vendors continue to develop new...

...impediments. FRET has been used in many applications, including drug discovery by big pharma (i.e. HTRF and LanthaScreen...dynamic range of your assay. These experiments include producing a positive control construct where the... Women’s Hospital in Boston, MA. His interests include developing novel fluorescent tools for labeling...

...2014, tools began moving away from designing exclusively for NGG PAM targets and began allowing for arbitrary... and return later or share them. Community exclusivity: Different communities develop software for their...software is organism agnostic. Most tools are exclusive to one or a subset of organisms. Examples: “organism...

...Scorpions Low Probe is on a hairpin structure included in the 5’ primer Yes Yes. Agarose gel  ...Low Reporter and quencher on hairpin structure included in primer Yes Yes Agarose gel Table...well-to-well normalization of signal. They are often included in commercial sets and most machines have a protocol...

...find the original publication and ensure that it includes validation data relevant to your application —... makes the choice easy! Protein-based blockers include dry non-fat milk or serums like FBS, PBS, and BSA...typically spans 2–5 orders of magnitude, which includes a wide dynamic range for fluorescent proteins....

...Addgene tiene una gran audiencia internacional, incluido trabajando con distribuidores de todo el mundo...  Addgene has a large international audience, including working with distributors across the world to ...

...engine May include validation data CiteAb Any Any Search engine May include validation data...

... there! Completed blog posts are also great to include on CVs and Resumes. An Expanded Network - Addgene...many different people. Recent Guest Writers Have Included: Wenning Qin, Molecular biologist with Biogen ...

...hunts. In fact, they’ve been following a set of clues that has led them to their biggest discovery yet...name! Additionally, anyone whose submission is included in the final four will receive some rockin’ newly...