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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 802 results for: Clu

Showing: 301 - 320 of 802 results

  1. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ...resources needed to accomplish your science, and can include: equipment, reagents, animals, glassware, and even...the institution/funding source. Major equipment includes large centrifuges, sequencers and analyzers, and... as many of these tools as possible, this even includes clocks, timers, and phones.  As for reagents and...

  2. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...AAVs to broadly spread throughout an organism, including difficult-to-target tissues like the central nervous...AAV pseudotyping. It has broad tissue tropism, including liver, muscle, lung, CNS, kidney, retinal, and...serotypes used to target the central nervous system, including neurons, AAV5 is generally the best choice for...

  3. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    .... Other practices that fall into this category include performing experiments with too few replicates ...institute better experimental design practices including talking to statisticians when necessary and encouraging...Hines Minisymposium on Reproducibility Booklet - Including descriptions of many organizations promoting reproducibility...

  4. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...phenotype in order to conclude that the phenotype is due to an on-target effect. Conclusions Selection of gRNAs...modification of interest, it would be unwise to make conclusions on the basis of the activity of a single gRNA...

  5. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ... cascades (reviewed in Abreu & Levitz, 2020), including light-sensitive chimeric GPCRS (OptoXRs) (Tichy...experiments!  First, closely read the publication, including the supplemental data. You’re most likely to find...hardware, optogenetics tools, viruses, and more.  Conclusion   Optogenetic tools provide a powerful way to...

  6. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ... variants that are less sensitive to ethanol, including the “middle” ADH1 promoter pADH1m, which maintains...Liqun Luo’s lab developed the Q system from a gene cluster in the fungus Neurospora crassa. The QUAS promoter...3 in a single modular vector for ease of use! Conclusion This post contains only a brief overview of some...

  7. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ...mentioned above. The initial IMP cohort of 78 mentees included: 47 women (60.2%) 20 graduate students (25.6%...directly attributable to participation in the program included improvements in writing fellowships, giving oral...impressions of the IMP experience. Conference highlights included remarks by the Spanish Secretary of Research, ...

  8. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    ...reproducibility of experiments and the validity of conclusions. Antibody engineering also benefits antibody-...antibody. (B) Examples of engineered antibodies, including chimerization, conjugation, and antibody fragments...There are different ways to achieve this goal, including error-prone PCR, degenerate primers, gene shuffling...

  9. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ..., 1998), this 15 amino acid peptide contains a cluster of four cysteines (CCXXCC, where X is any non-cysteine...with minimal cross-reactivity. Other strategies include using engineered ligases to attach a ligand to ...choosing a dye or ligand to use for your experiment. Conclusion Researchers and vendors continue to develop new...

  10. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    ...this time, it is! Factorial ANOVAs include two-way ANOVAs but can include many more than two independent ... Of course, a one-tailed test also works for exclusively less-than comparisons. Paired and unpaired t-tests...

  11. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ...Wild-type A549 cells do not express CAs9 and are included as a control (WT). Vinculin is a loading control... assay before, consider following their tips, including: Before digesting the DNA duplexes with Surveyor...PAGE instead of agarose to get sharper bands. Conclusion Overall, making a human cell line with a gene...

  13. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    ...Science is about fighting scientific partisanship, including more scientists in our leadership, and raising... Anschutz Medical Campus.  He studies viruses including HIV and is interested in global health policy....Committee about climate change. All the scientists – including global warming skeptics – insisted that more data...

  14. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ...on immune system Conventional cancer therapies include radiation therapy (owing to contributions from ...blocking. Other molecular targets under research include TIM-3, LAG-3, STAT-3, EGFR, CD20 and CD73 on specific... factors researchers are beginning to focus on include: Cell heterogeneity Tumors are composed of a wide...

  15. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...impediments. FRET has been used in many applications, including drug discovery by big pharma (i.e. HTRF and LanthaScreen...dynamic range of your assay. These experiments include producing a positive control construct where the... Women’s Hospital in Boston, MA. His interests include developing novel fluorescent tools for labeling...

  17. Deep Dive: qPCR

    Type
    Blog Post
    ...Scorpions Low Probe is on a hairpin structure included in the 5’ primer Yes Yes. Agarose gel  ...Low Reporter and quencher on hairpin structure included in primer Yes Yes Agarose gel Table...well-to-well normalization of signal. They are often included in commercial sets and most machines have a protocol...

  18. CRISPR 101 eBook...en español

    Type
    Blog Post
    ...Addgene tiene una gran audiencia internacional, incluido trabajando con distribuidores de todo el mundo...  Addgene has a large international audience, including working with distributors across the world to ...

  19. Technical Design of a Western Blot

    Type
    Blog Post
    ...find the original publication and ensure that it includes validation data relevant to your application —... makes the choice easy! Protein-based blockers include dry non-fat milk or serums like FBS, PBS, and BSA...typically spans 2–5 orders of magnitude, which includes a wide dynamic range for fluorescent proteins....
Showing: 301 - 320 of 802 results