We narrowed to 443 results for: DUR

1. Lab Automation at Addgene

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   Blog Post

   ...pipette samples in and out of it, you can set up a procedure for it using the Biomek FxP. For example, we added...

2. Plasmids for Endogenous Gene Tagging in Human Cells

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   ...plasmid from being cut and destroyed by CRISPR/Cas9 during transfection. Making the double strand break and...

3. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

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   ...scientists will contact you if any issues arise during the QC process. As the depositing scientist, you...

4. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

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   ...regulation. This can be used to activate transcription during either initiation or elongation, depending on which...

5. Meet an Addgenie: Insights into Grad School, Career Paths, and More

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   ...it solidified some concepts that are heard often during undergrad. Lauren Flynn is the Addgenie behind...

6. Viral Vectors 101: Chemogenetics

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   ...Carpenter JC, Snowball A, Knauss S, von Schimmelmann M, During MJ, Lignani G, Schorge S, Young D, Kullmann DM,...

7. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

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   Blog Post

   ...that site. So, when DNMT1 methylates the genome during DNA replication, it doesn’t methylate sites blocked...

8. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

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   ...the protein-antigen complexes will be washed away during the protocol. The amount of antibody that binds...

9. 6 Tips for Grant Writing

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   ...’s a good idea to move around to different labs during your career; you learn new techniques and interact...

10. Tips for Titering Your Lentiviral Preps

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    ...subcloning, expansion, and confirmation. This procedure can take several months but should be considered...

11. Illuminating Epigenetics with A FRET Based Biosensor

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    ... and diabetes mortality determined by nutrition during parents' and grandparents' slow growth period."...

12. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

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    ...molded the whole thing into a marketable product. During this process, we had an idea that survived the ...

13. Hot Plasmids: Winter 2025

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    ... inserted. B) Protein expression and labeling procedure. Adapted from Smith et al. 2024 under a CC-BY ...

14. Degrading DNA with Cascade-Cas3

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    ...more gRNAs must be used along the same chromosome. During DNA repair, there is a chance that the two breakpoints...

15. Antibodies 101: Flow Compensation

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    ... you can always change your compensation values during the analysis portion of your experiment. So, as...

16. Twenty Years of Addgene Sharing: CRISPR

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    Blog Post

    ...the usefulness of popular CRISPR tools is both enduring and rapidly recognized.   Where is the CRISPR ...

17. Transferable Skills Guide: Managing a Team

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    ...mine you didn’t think much about management skills during your scientific training. I never thought about...

18. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

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    ...translated protein (sybody), ribosome, and mRNA. During the screening process, displayed sybodies that ...

19. Transferable Skills: Negotiation

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    ...you are negotiating. Then take a moment before, during, or after a negotiation to think about the situation...

20. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

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    ...mass bleaching, the loss of their symbiotic algae, during heat stress. For other organisms, like the sea ...