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We narrowed to 756 results for: ELP

Showing: 301 - 320 of 756 results

  1. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ...largely does not integrate, and the addition of a helper virus or plasmid allows it to replicate episomally...research, you might have an idea which ones could be helpful to you. Interested in learning more about a specific...

  2. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ... Stable, Stbl3, JC8111, SURE2, or XL10-Gold will help to reduce the likelihood of ITR deletions occurring...sequencing results. Next-generation sequencing is also helpful in quantifying ITR mutants since analysis of individual...

  3. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    ...the DNA surrounding your restriction site(s) can help you quickly predict which (if any) of those sites...visualization software (we used Snapgene here) can help you assess whether your restriction site will be...

  4. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    ...field will know about grants and projects that might help your work Tenure can depend on your national recognition... and be ready to talk about them. People love to help especially when solving problems.  During the conference...

  5. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    ...these measurements and hope this information can help guide your understanding of reported data. Photobleaching...substantial changes in excitation power and will help to keep the photostability of the probes in check...

  7. RNA Extraction Without A Kit

    Type
    Blog Post
    ...biotechnology companies have marketed several products that help to further streamline this process and/or stabilize...choose from) on your resuspended RNA sample will help ensure DNA contamination is not an issue.  References...

  9. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    ...we wish to conduct research that fails less and helps more people, we need to take sex into account. Gendered...Physiology over the past decade. For more practical help, there are numerous methodological articles to consider—we...

  10. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    ...can use primer design software such as Primer3 to help you design your custom primers. Remember, when designing...forward and reverse primers for each sample with the help of DNA-editing software which integrates our database...

  12. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...the practical application of the plasmid and will help a scientist outside your field understand if this...up-to-date version! We hope the deposit spreadsheet will help expedite your data entry and make it easier than...

  14. A Look at Addgene's QC Process

    Type
    Blog Post
    ...it be fully sequenced by Addgene’s QC team. Email help@addgene.org to get started! Addgene’s QC process...the raw NGS reads to a reference sequence can also help QC scientists determine if enough reads were acquired...

  15. Design Tips for Prime Editing

    Type
    Blog Post
    ...details. You can find many software programs to help you design pegRNAs. Here on the Addgene blog, we...a guest blog by the developer of PRIDICT, which helps users select pegRNA designs that are most likely...

  18. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...family Parvoviridae and requires co-infection of helper viruses like adenovirus for productive infection...and the selection of specific promoters may also help AAV vectors evade host immunity. While basic research...

  19. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...America. Diagnostics will play an important role in helping to monitor and slow this spread until vaccine programs...toehold switches and amplification primers. This helped make sure that we had a number of successful sensors...

  20. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ...Sticky-end mediated gene transfer would be particularly helpful for targeting non-dividing cells, which are difficult...Exploring the efficiency of these two processes will help us better understand where Cpf1 fits into the genome...
Showing: 301 - 320 of 756 results