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We narrowed to 650 results for: EPO

Showing: 301 - 320 of 650 results

  1. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...sections of DNA can be more difficult. Addgene depositors have developed new CRISPR-based methods for various...or cell lines, please consider sharing them by depositing with Addgene! Figures were adapted from: Hall...

  2. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ... nuclease active site wasn’t even close to its reported cleavage site on the complementary DNA strand....model also displays the several Lys/Ala mutations reported by Ian Slaymaker and colleagues to reduce off-...

  3. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...easier to construct targeting vectors. Addgene depositor Takashi Yamamoto’s lab has harnessed MMEJ to create...Pages Find CRISPR Plasmids for Your Experiments Deposit Your New Plasmid Tools with Us  ...

  4. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...diseases and metabolic diseases are also underway and reported results are encouraging. Importantly, a significant...combined with AAV for in vivo gene editing, and reports suggest that the first clinical study combining...

  5. An Introduction to Adenovirus

    Type
    Blog Post
    ...(Tollefson et al., 1996) – cell death has been reported when using vectors, and immune inactivation of...pre-existing toolbox for adenoviral vectors in Addgene’s repository is smaller than for other vector types, we hope...

  6. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...experimental settings. It is a curated set of technical reports on AAV vectors from Addgene and the CLOVER Center...Center, each one submitted by researchers like you. Reports are reviewed by Addgene scientists for clarity ...

  7. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...viral vector system that could do both, Addgene depositor Didier Trono and collaborators turned to the lentivirus...transduced rat neurons in vivo with beta-gal and other reporters, and they showed that expression persisted for...

  8. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ...in mammalian cells, LbCpf1 and AsCpf1. In both reports, on target editing efficiency for the Cpf1 orthologs...mismatches, but variably so, with Kleinstiver et al. reporting that Cpf1 can tolerate mismatches at gRNA positions...

  10. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...even been linked to some diseases. It has been reported that 5-10% of human genes contain a region where...EMBO Rep 1:18–23 . https://doi.org/10.1093/embo-reports/kvd001 Additional resources on the Addgene blog...

  11. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...Hu et al., 2018).  In 2020, the Kleinstiver lab reported the development of near-PAMless Cas9 variants....Streptococcus thermophilus (St) NNAGAAW Treponema denticola (Td) NAAAAC ~20 additional Cas9...

  13. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    .... Specifically, efficacy rates of Cas7-11 were reported as up to 85%, broadly similar to shRNA and Cas13...However, in those same comparisons, the AbuGoot lab reported no detectable effect on cell viability – meaning...

  14. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ... inactivated by the complement system in serum (DePolo et al., 2000). Other envelope proteins for pseudotyping...–398 . https://doi.org/10.2174/1566523054546224 DePolo NJ, Reed JD, Sheridan PL, Townsend K, Sauter SL...

  15. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...of interest and an inactivated fluorescent RNA reporter. If the target sequence is present in the pool...activity of Cas13a becomes activated and the RNA reporter will be cleaved resulting in activation of the...

  16. How to Write a Scientific Review Article

    Type
    Blog Post
    ... member of a class of proteins was mutated, as reported in various studies. We made a table that listed...for each protein we listed all the studies that reported mutations in that protein (including how frequently...

  17. Hot Plasmids - November 2023

    Type
    Blog Post
    ... plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles. Today, it'...or inaccessible. The Ting lab recently made two deposits that increase the utility of this technique.  ...

  18. Viral Production at Addgene

    Type
    Blog Post
    ...expression and/or function. These data are sometimes reported or posted on the material page for the corresponding...recipient’s initial thaw will be accounted for in our reported titers. Lentiviral vectors are titered using a...

  19. Hot Plasmids Spring 2024

    Type
    Blog Post
    ... plasmids, antibodies, and viral preps in our repository through our Hot Plasmids articles.  Here's ...antibodies were recently added to the Addgene repository of tools for SAR-CoV-2 research. Two new antibodies...

  20. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...single-use aliquots, and stored at -80°C. Some reports suggest rapidly freezing virus in a dry ice/ethanol...Things Lentivirus with our Lentiviral Guide Pages Deposit Your Lentiviral Vectors ...
Showing: 301 - 320 of 650 results