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We narrowed to 655 results for: EPO

Showing: 301 - 320 of 655 results
  1. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...they are integrating viruses – they physically deposit their cargo into the host’s DNA. These viruses ...familiarize yourself with your organization’s reporting mechanisms before you start an experiment, just...
  2. Tips for Technical Support Calls

    Type
    Blog Post
    ...the experiments performed by our thousands of depositors. That means their original articles are your ... Addgene Help Center Get Help with the Addgene Deposit Process Resources on Addgene.org Check Out Our...
  3. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ...in mammalian cells, LbCpf1 and AsCpf1. In both reports, on target editing efficiency for the Cpf1 orthologs...mismatches, but variably so, with Kleinstiver et al. reporting that Cpf1 can tolerate mismatches at gRNA positions...
  4. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to ...
  5. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...sensors are better classified by their fluorescent reporter and the mechanism of their voltage sensing component...genetic cargo in lieu of their own genomes and deposit this cargo upon host infection. Packaging viruses...
  6. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...have been around since 2000 when Terskikh et al. reported the production of dsRed E5. This timer predictably...fluorescent timers that change color from blue to red report on cellular trafficking." Nature chemical biology...
  7. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...sections of DNA can be more difficult. Addgene depositors have developed new CRISPR-based methods for various...or cell lines, please consider sharing them by depositing with Addgene! Figures were adapted from: Hall...
  8. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ... nuclease active site wasn’t even close to its reported cleavage site on the complementary DNA strand....model also displays the several Lys/Ala mutations reported by Ian Slaymaker and colleagues to reduce off-...
  9. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ... In our study, we used the TRIP (Thousands of Reporters Integrated in Parallel; Akhtar et al., 2013; Schep...Chromatin Position Effects Assayed by Thousands of Reporters Integrated in Parallel. Cell, 154(4), 914–927....
  10. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...easier to construct targeting vectors. Addgene depositor Takashi Yamamoto’s lab has harnessed MMEJ to create...Pages Find CRISPR Plasmids for Your Experiments Deposit Your New Plasmid Tools with Us  ...
  11. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...experimental settings. It is a curated set of technical reports on AAV vectors from Addgene and the CLOVER Center...Center, each one submitted by researchers like you. Reports are reviewed by Addgene scientists for clarity ...
  12. An Introduction to Adenovirus

    Type
    Blog Post
    ...(Tollefson et al., 1996) – cell death has been reported when using vectors, and immune inactivation of...pre-existing toolbox for adenoviral vectors in Addgene’s repository is smaller than for other vector types, we hope...
  13. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...diseases and metabolic diseases are also underway and reported results are encouraging. Importantly, a significant...combined with AAV for in vivo gene editing, and reports suggest that the first clinical study combining...
  14. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...viral vector system that could do both, Addgene depositor Didier Trono and collaborators turned to the lentivirus...transduced rat neurons in vivo with beta-gal and other reporters, and they showed that expression persisted for...
  15. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ...in mammalian cells, LbCpf1 and AsCpf1. In both reports, on target editing efficiency for the Cpf1 orthologs...mismatches, but variably so, with Kleinstiver et al. reporting that Cpf1 can tolerate mismatches at gRNA positions...
  16. Promega Plasmid Collection

    Type
    Collection
    ... and more. Luciferase Reporters Luciferase (Link opens in a new window) reporter assays use light produced...Find luciferase reporter vectors for sensitive gene expression analysis and fusion vectors containing... Depositor Collections Promega Plasmid Collection Promega... workflows. The collection contains luciferase reporter vectors for sensitive gene expression analysis...collection. For example, search by response element, reporter, protein, or tag, such as "NFAT", "luciferase"... a new window) is a structural complementation reporter system composed of a Large BiT (LgBiT; 18 kDa)...Addgene Find additional information about luciferase reporter assays (Link opens in a new window) from Promega...
Showing: 301 - 320 of 655 results