We narrowed to 610 results for: GAN
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TypeBlog Post...investigate the susceptibility of cells from different organisms to SARS-CoV-2 (Chu et al., 2020). An additional...mechanisms to maintain and grow these critical organizations should be a fundamental part of the scientific...
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Networking Follow-up: I Met a Person. Now What?
TypeBlog Post...If appropriate, invite them to speak for your organization, mentoring group, or department. Even if it ...gestures are memorable! Attend events for one organization or go to the same conferences/meetings over ... -
New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor
TypeBlog Post... iGluSnFR could detect neuronal activity in C. elegans, zebrafish, and mice. Second Generation iGluSnFR...C-terminal domain of Stargazin including a terminal PDZ ligand. The researchers recommend using the SGZ for... -
Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish
TypeBlog Post...extreme life on earth including extremophile microorganisms, biomarkers, and recently, cancer research ...Addgene has partnered with various community organizations and workforce projects to reach audiences beyond... -
FPbase: A new community-editable fluorescent protein database
TypeBlog Post...that selection of a fluorescent probe (be it an organic dye or FP) is one of the most important steps in...tools. Years ago, Kurt Thorn (UCSF at the time) began an effort to collate some of the current “best” ... -
Science Career Options
TypeBlog Post...that requires travel can show that you are self organised, reliable, and can work independently. Practical...involves traveling, showing that you are a highly organised and experienced traveler by citing specific examples... -
With Our New Viral Service, We're Taking CRISPR Further!
TypeBlog Post... number of CRISPR plasmids for that particular organism available at Addgene. Percentages represent the...used to deliver CRISPR components to cells or organisms under study. In the past we’ve left the arduous... -
How to Lead a Great Meeting
TypeBlog Post... are needed. All-Company Meetings: As your organization grows, so will the length of your group meetings... strategies and find out what works for your organization. Ask for feedback from your colleagues. When... -
Components of CRISPR/Cas9
TypeBlog Post... cut the target. Cas9 homologs from different organisms or Cas9 mutants developed in a variety of labs...EQR variant 3' NGAG SpCas9 VQR variant 3' NGAN or NGNG xCas9 3' NG, GAA, or GAT SpCas9-NG ... -
Management for Scientists: Delegating is Key
TypeBlog Post...develop to reach their full potential in the organization. This will result in more engaged, successful...Series Learn How Volunteering at a Professional Organization Can Help You Develop Leadership Skils Set Concrete... -
Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity
TypeBlog Post...different organisms and engineered Cas9 variants that were able to target different PAM sites (NGG, NGAN, NGNG... -
Viral Vectors 101: The AAV Retrograde Serotype
TypeBlog Post... environment, all while reciting their favorite slogan, “look how cool the brain is!" Using retrograde...account since activation with chemogenetic specific ligands activates both neurons at the injection site and... -
Plasmids 101: Screens vs. Selections
TypeBlog Post...mutagenizing a population (typically cells or small organisms) and then assaying for a functional or phenotypical... events. To put it another way, only cells or organisms with the desired phenotype will survive and be... -
AAV Q&A with Tim Miles
TypeBlog Post...systemically transduce and then retransduce a model organism such as mice, e.g. first at 2 months, then at ...field has settled on ‘best practices’ for model organisms. Q11: How consistent is expression is when ... -
Plasmids 101: Inducible Promoters
TypeBlog Post...for easy genome engineering in species like C. elegans and Drosophila. Light inducible promoters Light...transcription machinery differs between cell types or organisms, promoters are similarly variable. Bacterial promoters... -
A Novice's Perspective on Neuroscience - SfN2017
TypeBlog Post...Neuroscience covers many subfields When I first began looking into the details of SfN17, I expected that...addition, there was an entire story-telling session organized by Story Collider and which featured a talk about... -
Why and how to start studying chromatin (with Addgene's help)
TypeBlog Post... 2018 Chromosome Architecture and Chromosome Organization Keystone Symposium. As it happens, there are...Keystone Chromosome Architecture and Chromosome Organization. These aren’t the only investigators in the ... -
Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer
TypeBlog Post...inflammation for therapeutic benefit ”as stated by the organizers. My overall impression from this conference...dynamic microvillar search and stabilization during ligand detection by T cells." Science 356.6338 (2017):... -
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs
TypeBlog Post...processing machinery present in almost every living organism. Precursor, or pre-tRNAs are cleaved by RNase ... illustrated the power of polycistronic gene organization, and its translation to CRISPR/Cas9 shows great... -
Plasmids 101: The protein expression toolbox
TypeBlog Post...the relevant tag and then introducing a specific ligand to mark the tag and whatever it is fused to for...expressed, protein levels will quickly recover once the ligand is removed. Degron tag systems include small molecule...