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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 614 results for: GAN

Showing: 301 - 320 of 614 results

  2. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ...tools by increasing gRNA stability. First, they began with the Broccoli aptamer system to visualize the... will recruit the HaloTag with red fluorescent ligands to the loci of interest. Dual color detection In...

  3. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    ... iGluSnFR could detect neuronal activity in C. elegans, zebrafish, and mice. Second Generation iGluSnFR...C-terminal domain of Stargazin including a terminal PDZ ligand. The researchers recommend using the SGZ for...

  4. Science Career Options

    Type
    Blog Post
    ...that requires travel can show that you are self organised, reliable, and can work independently. Practical...involves traveling, showing that you are a highly organised and experienced traveler by citing specific examples...

  5. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    ... number of CRISPR plasmids for that particular organism available at Addgene. Percentages represent the...used to deliver CRISPR components to cells or organisms under study. In the past we’ve left the arduous...

  7. How to Lead a Great Meeting

    Type
    Blog Post
    ... are needed. All-Company Meetings: As your organization grows, so will the length of your group meetings... strategies and find out what works for your organization. Ask for feedback from your colleagues. When...

  8. Components of CRISPR/Cas9

    Type
    Blog Post
    ... cut the target. Cas9 homologs from different organisms or Cas9 mutants developed in a variety of labs...EQR variant 3' NGAG SpCas9 VQR variant  3' NGAN or NGNG  xCas9 3' NG, GAA, or GAT SpCas9-NG ...

  9. Management for Scientists: Delegating is Key

    Type
    Blog Post
    ...develop to reach their full potential in the organization. This will result in more engaged, successful...Series Learn How Volunteering at a Professional Organization Can Help You Develop Leadership Skils Set Concrete...

  12. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    ...mutagenizing a population (typically cells or small organisms) and then assaying for a functional or phenotypical... events. To put it another way, only cells or organisms with the desired phenotype will survive and be...

  13. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...systemically transduce and then retransduce a model organism such as mice, e.g. first at 2 months, then at ...field has settled on ‘best practices’ for model organisms.    Q11: How consistent is expression is when ...

  15. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ... environment, all while reciting their favorite slogan, “look how cool the brain is!" Using retrograde...account since activation with chemogenetic specific ligands activates both neurons at the injection site and...

  16. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ...for easy genome engineering in species like C. elegans and Drosophila. Light inducible promoters Light...transcription machinery differs between cell types or organisms, promoters are similarly variable. Bacterial promoters...

  17. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ...Neuroscience covers many subfields When I first began looking into the details of SfN17, I expected that...addition, there was an entire story-telling session organized by Story Collider and which featured a talk about...

  18. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...processing machinery present in almost every living organism. Precursor, or pre-tRNAs are cleaved by RNase ... illustrated the power of polycistronic gene organization, and its translation to CRISPR/Cas9 shows great...

  20. RNA Extraction Without A Kit

    Type
    Blog Post
    ...proteins are retained in the interphase and bottom, organic layer. The top aqueous layer is extracted and RNA...layer to avoid carryover of DNA from the bottom, organic layer. Another trick is to precipitate the RNA ...
Showing: 301 - 320 of 614 results