Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 12 results for: GAN

Showing: 1 - 12 of 12 results

  1. Chemogenetics Guide

    Type
    Guide
    ...small molecule ligand. The ideal chemogenetic tools are unresponsive to native ligands and are engineered...transmembrane proteins that bind a specific ligand. Ligand binding then activates G-proteins to modulate...unresponsive to endogenous ligands, could be activated by synthetic ligands. Specifically, Receptors Activated...insensitivity to endogenous ligands, have low constitutive activity, and their activating ligands have few, if any... Figure 2: DREADDs, their ligands, and signaling properties. DREADD Ligands Muscarinic-receptor based ...neurotransmission. This, and the fact that GPCR ligands can have high potency, which is potentially important... Activated Solely by Synthetic Ligands (RASSLs) based on κ-opiod receptors (Coward et al. 1998). These...

  2. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...AAV vectors . Microorganisms, 12 (2), 384. https://doi.org/10.3390/microorganisms12020384 (Link opens in...genes. rAAV episomes can develop chromatin-like organization and persist in non-dividing cells for a period...different serotype(s) for transduction of a given organ. Tissue Serotype Central nervous system AAV1, AAV2...administration, distributing widely throughout the organism to target large or diffused biological structures... of systemic rAAV vectors that can target whole organs or specific cell types of the CNS (e.g. AAV-PHP.eB... transport Movement of molecules, vesicles or organelles from the main cell body, or soma, towards the...pathogen, like viruses or bacteria, within a host organism, leading to disease. Insertional mutagenesis Random...

  3. Molecular Biology Reference

    Type
    Guide
    ...transcriptional machinery from a particular organism or group of organisms. Meaning, if a plasmid in intended ...abbreviated to A, T, C, and G respectively) that are organized into a double stranded helix. The order of these...the instructions to make every protein within an organism. Proteins are made up of amino acids. Each amino...can drive gene expression in a wide variety of organisms, including plants, worms, mice, and even cultured...when trying to sequence an entire plasmid or an organism’s genome. One Sanger sequencing reaction would ...

  4. Modular Cloning Guide

    Type
    Guide
    ...at 18 levels for rapid characterization in new organisms. Mobius Assembly Vector Toolkit Bacterial Expression...reconstituting the ubiquitination cascades of different organisms in E. coli . MetClo DNA Assembly Kit Bacterial...Platinum TALENs for use in a wide variety of target organisms. TALEN Kit Genome Engineering, TALEN Stephen Ekker...fusion proteins for use in many different model organisms. Diatom uLoop Assembly Kit Other, Yeast Expression...tags, and signal peptides, for use in a range of organisms including diatoms, yeast, plants and bacteria....

  5. CRISPR Guide

    Type
    Guide
    ...knock out target genes in various cell types and organisms, but modifications to various Cas enzymes have...be added to detect the presence of infectious organisms (like SARS-CoV-2 ) and genetic mutations. Similar...SpCas9 EQR variant 3' NGAG SpCas9 VQR variant 3' NGAN or NGNG xCas9 3' NG, GAA, or GAT SpCas9-NG 3' NG...Hultquist, J. F., Waters, C. S., McGregor, M. J., Krogan, N. J., & Bondy-Denomy, J. (2017). Inhibition of..., E. P. R., Lin, S., Kiani, S., Guzman, C. D., Wiegand, D. J., Ter-Ovanesyan, D., Braff, J. L., Davidsohn...23907171 Nuñez, J. K., Chen, J., Pommier, G. C., Cogan, J. Z., Replogle, J. M., Adriaens, C., Ramadoss,...

  6. Optogenetics Guide

    Type
    Guide
    ...and study certain behavioral responses in model organisms like mice, zebrafish, and Drosophila. These tools...LAD Light-activated dimerization, using proteins GIGANTEA (GI) and the light oxygen voltage (LOV) domain...engineered zinc finger proteins, using proteins GIGANTEA (GI) and the light oxygen voltage (LOV) domain...

  7. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...Hussain, R., Banani, M. A., Day, W., Stevenson, E., Madigan, M., Chen, J., Khinder, R., . . . Pule, M. (2022...pathogen, like viruses or bacteria, within a host organism, leading to disease. Provirus The genetic material...

  8. Plan Your Experiment

    Type
    Guide
    ...researchers can adapt these experiments for different organisms. This page provides a general framework to get...principles also apply to using CRISPR in other organisms. As with most experiments, you first need to have...

  9. Lentiviral Vector Guide

    Type
    Guide
    ... Naldini, L., Blömer, U., Gallay, P., Ory, D., Mulligan, R., Gage, F. H., Verma, I. M., & Trono, D. (1996...pathogen, like viruses or bacteria, within a host organism, leading to disease. Lentivirus A retrovirus from...

  10. Antibody Guide

    Type
    Guide
    ...myeloma cells (immortalized B cells). The genetic reorganization that creates variety in the antibody allowing...the variable region goes through a randomized reorganization process, creating a unique binding site. This...

  11. Promoters

    Type
    Guide
    ...promoter region controls when and where in the organism your gene of interest is expressed. Summary Promoters...

  12. Adenovirus Guide

    Type
    Guide
    ...pathogen, like viruses or bacteria, within a host organism, leading to disease. Innate immunity Non-specific...
Showing: 1 - 12 of 12 results