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We narrowed to 1,144 results for: Gene

Showing: 301 - 320 of 1144 results

  1. Top Requested AAV of 2017: pmSyn1-EBFP-CRE

    Type
    Blog Post
    ...Cre-dependent gene of interest in neurons. This ability to be paired with any Cre-controlled gene of interest...neuroscientists because they efficiently deliver genes to various brain regions. You can partially control...occasionally you may want to further control where your gene of interest is expressed.  A cell-type specific ...and what do they mean? AAV: A Versatile Tool for Gene Expression in Mammals Subscribe to Viral Vector ...! To find more viral vectors available through Addgene, be sure to check out our viral service page and...Vector Blog Posts Additional Resources on Addgene.org Check out our Viral Service Page Browse Additional ...

  2. MAPK Plasmids

    Type
    Collection
    ...deposit@addgene.org to be paired with an Addgene scientist. MAPK Plasmids (organized by gene) Gene Alternate... numerous research labs from around the globe, Addgene has assembled a MAPK plasmid collection for community...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  3. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    ...inducible yet permanent gene excision. Essentially, LoxP sites are knocked in flanking a gene of interest (or ... the gene for your protein of interest. CRISPR-Cas9 and a custom gRNA is all you need to generate a frameshift...? Is the regulation of an exogenously introduced gene or over the native locus? Let’s explore each of...Cas9 fused to a transcriptional repressor to your gene of interest. It will inhibit transcription instead...cinch. Stably introducing the coding region of a gene (cDNA) can be done by either plasmid or virus. A...Cas9. The system is led, via a custom gRNA, to your gene of interest to temporarily increase expression. ...of action Check out Addgene's Lentiviral Tet-on and AAVS1 targeted Tet-on transgene vector. Degron tags...

  4. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    ...all known genes. This is a perfect example of an unbiased, reverse screen – all known genes are assayed...of knocking out the genes of interest (or the whole genome), they knock down gene expression temporarily... a biological process. Players can be proteins, genes, enzymes, drugs, or anything else that plays a role...where there is removal of known factors (such as a genes) and the loss of those factors is assessed. The ...especially useful if you are interested in studying genes whose knockout would be lethal to cells and thus...The two most common methods of manipulation are genetic knockout mutations and treatment with small molecules...selection would work is if you are trying to identify genetic conditions which convey resistance to a drug. You...

  5. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ...stably integrate a reporter gene that contains a puromycin resistance gene followed by the target site...that targets your desired gene (whose sequence is also found in the reporter gene). A control RFP expression...donor plasmid that contains a puromycin resistance gene linked to an in-frame H2B-GFP. This visual read ... express RFP while cells containing the reporter gene that was cleaved by Cas9 and repaired via HDR using...unedited cells. In an experiment that targeted the gene NFE2L2, the Madison lab found that H2B-GFP expressing...desired genomic edit.  Find SRIRACCHA plasmids at Addgene GEMCherry2, an improved monomeric fluorescent protein...stem cells.  Find GEmCherry2 reporter plasmids at Addgene Overall SRIRACHA and GEmCherry2 reporter systems...

  6. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type
    Blog Post
    ... allowing for the gene on the toehold switch to be expressed. In this case, the gene encodes the LacZ𝛼...interfere with other genetic pathways making it difficult to link a phenotype to the gene being targeted via...need for mutant genetic backgrounds. The standard RNAi vector, L4440, places a target gene between two inverted..., highly efficient RNAi construct for C. elegans gene knockdown Article contributed by Alyssa Cecchetelli...extensively used in C. elegans research to study gene function. RNAi is commonly achieved by feeding E... from each promoter progresses past the specific gene producing long unspecific RNA fragments that are... most scientists use mutant animals that are genetically altered to overexpress endogenous RNAi pathways...

  7. Working with Nuclear Receptors

    Type
    Blog Post
    ...functional groups of genes involved in the execution of a specific regulatory regime (such as genes involved in...consortium-generated datasets with a systematic effort to compile NR- and coregulator-related global gene profiling...remain unknown. Together, NRs govern expression of genes involved in a broad range of reproductive, developmental...efficient and timely activation or repression of gene expression (see www.nursa.org for a curated list... NRs and coregulators, and although the reporter gene has over the years evolved from chloramphenicol ...O'Malley, B. W. (2002). Combinatorial control of gene expression by nuclear receptors and coregulators...funding model in nuclear receptor research, and has generated a set of comprehensive datasets that collectively...

  8. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    ...specific genes involved. Downregulation of tumor suppressors and upregulation of proto-oncogenes ­­­can ...click on a gene of interest will direct the investigator to a list of all available Addgene plasmids he... group of genes/proteins that, when expressed, normally encourage cell survival, growth and proliferation... the health of an organism that both classes of genes function normally to maintain balance – encouraging...Technologies, Carlsbad, CA) that represents 180 genes from the Ras signaling pathway. Its major appeal...of vectors to study the function of any of these genes through several different applications such as fluorescent... in the development of cancer. With hundreds of genes involved in the Ras pathway alone and thousands ...

  9. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...harnessed MMEJ to create a new method for CRISPR gene knock-in, termed PITCh (Precise Integration into Target ...than that observed with NHEJ. PITCh: Using MMEJ for gene knock-in Building on the lab’s previous work, Sakuma... should target near the last coding exon of your gene of interest. For ideal use, this gRNA should be ... is if the GFP-Puro will alter expression of the gene it follows. For increased versatility, it would ...would be advantageous to adapt PITCh to insert genes into AAVS1, the “safe harbor locus” of the human genome...another strategy researchers can exploit for CRISPR gene editing. In organisms where HDR is downregulated...Sakuma, Tatsushi, et al. (2016).  “MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh...

  10. CRISPR References and Information

    Type
    Collection
    ...identify positively or negatively selected sgRNAs, genes, or pathways. For use with genome-scale CRISPR-Cas9...CRISPR screens targeting a long or short list of genes provided by the user. Developed by the Fredrik Wermeling...sgRNA design tool for genome editing, as well as gene regulation (repression and activation). Genome support...target sites by off-target quality. Validated for gene inactivation, NHEJ, and HDR. Includes a wide range... to identify novel gRNA target sites in a single gene, as well as a target site common to a set of similar... Information Addgene Resources CRISPR Software General Resources CRISPR Protocols Addgene CRISPR Resources...Resources CRISPR Plasmids Addgene's collection of CRISPR plasmids. CRISPR Guide Addgene's guide to using CRISPR...

  11. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

    Type
    Blog Post
    ...hygromycin resistance gene as the plant-selectable marker and a kanamycin resistance gene for bacterial selection...viral vectors have been successfully used to generate gene-edited plants without the need for tissue culture... viral delivery is a highly effective method for gene editing and allows targeting of specific cell types...demonstrated that TnpB can also function as an efficient gene activator when fused to a transcriptional activation...TnpB can be used to simultaneously target multiple genes, enabling efficient multiplexed genome editing in...Identify a TAM site (5′-TTGAT-3′) in your target gene. Choose a 20-nt sequence immediately downstream ...for delivery via viral vectors and for multiplexed gene editing applications. As research into TnpB continues...

  12. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    ... and the gene that is responsible is understood. It’s possible to actually silence this gene with RNAi...only 1,700 genes total). Hydra (Celina Juliano) This is a known stem cell and regeneration model. Little... human interaction for all of us studying human genes in test tubes and gels). As a freshman in college... I studied development and evolution of histone genes in Strongylocentrotus purpuratus, the spiny purple...direct tests of cause and effect hypotheses between gene function and phenotypes in diverse plants, animals...frog cell lines to develop robust methods for frog gene editing. They edit in a cell line, then take the...nonetheless - the first example was of a pigmentation gene.   Genus Microtus (Zoe Donaldson) Voles have fascinating...

  13. Feng Zhang Multiplexed Overexpression of Regulatory Factors (MORF) Collection

    Type
    Collection
    ...transcription factor collection that contains 1,836 genes encoded by 3,548 isoforms and two control vectors...10X scRNA-seq (PDF, 59.2 KB) Plasmids ID Plasmid Gene/Insert Pooled Library The MORF pooled library is... transcription factor collection, available at Addgene. The collection can be used for screening transcription... entire pooled collection, can be purchased at Addgene. Each transcription factor isoform has a unique...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  14. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    ...courses on genetic manipulation more hands on While teaching courses about gene therapy and genetic manipulation...that similar types of thinking go into developing gene therapy strategies for various diseases. That’s ...Inserts, Tags, Origins of Replication, Resistance gene and Selection Markers) and one “Transfer Plasmid...had to pick the best strategy and propose a final gene therapy approach for the disease to be openly discussed...impression was that they had clearer ideas about gene therapy in the following lessons. And let me tell...teach gamma-retrovirus or lentivirus-based ex-vivo gene therapy’ strategies to undergraduates, however you...problem is…how can you make a “hands-on” lesson on genetic manipulation of human stem cells when students ...

  15. An Addgene Summer Internship

    Type
    Blog Post
    ...choose what gene to focus on. The gene choice had restrictions. It couldn’t be a Covid gene, a toxin, or...Syntaxin3 gene was the winner. We used the next 4-5 weeks to work on cloning the Syntaxin3 gene in a pLenti...after discussion, we sent it for sequencing. The gene was successfully sequenced, confirming that we had...with Amy C., Addgene’s intern coordinator shortly after hearing her talk about Addgene’s internship opportunity... past six weeks. Through Addgene, I learned the basics of lab safety, general work skills — such as using...environment changes them. Addgene gave me the chance to become a real life scientist. Addgene gave me the chance...knowledge more than ever before. Before joining Addgene, I had spent most of my junior year stressed out...

  16. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ...systems allow researchers to understand bacterial gene clusters that produce useful natural materials, ...capable of robust expression of high G+C content genes, as validated through monitoring the expression ...human mRNA binding proteins with 10 guide RNAs per gene. The lab modified the lentiviral backbone to encode...pooled library from Junjie Chen's lab targets human genes known or suspected to be involved in DNA damage ...Veening is now available with one sgRNA per operon or gene. The sgRNA pool can also target core operons in ...library uses CRISPR cytosine base editors to disrupt gene function by targeting start codons, splice acceptor...find all of the CRISPR plasmids available from Addgene, head over to our CRISPR Plasmids and Resources...

  17. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    ... plasmids to generate deletions of candidate genes in euploid iPSCs. “The BeHEARD Addgene reagents will... lentiviral vectors from Addgene, the lab will express these candidate genes in TS iPSCs to identify whether...CTD, which arises due to mutations in the SLC6A8 gene, also known as the creatine transporter. The Association...Congratulations Mark Mellett! Siddharth Prakash: X chromosome genes involved in Turner syndrome Turner syndrome (TS)...can lead to cardiac developmental defects. Eight genes from the X chromosome are expressed at significantly...will facilitate our aims to correct X chromosome gene dosage in TS cells as a first step to reverse the...Germline mutations in one of these parts, the GNB1 gene, which codes for G𝛃1 subunits of G protein complexes...

  18. Promega Plasmid Collection

    Type
    Collection
    ...Find luciferase reporter vectors for sensitive gene expression analysis and fusion vectors containing...contains luciferase reporter vectors for sensitive gene expression analysis and protein fusion vectors for...tracking protein degradation, analyzing CRISPR-mediated gene editing outcomes, and conducting high-throughput...Promega — now available through Addgene. As a purpose-driven nonprofit, Addgene offers expert support, educational...Browse Addgene's Luciferase Plasmid Collection Explore other Promega plasmids available at Addgene Find ...subunits come together to form an active enzyme and generate a bright luminescent signal in the presence of...window) blogs for technical tips and tools for genetic reporter assays (Link opens in a new window) and...

  19. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...comparing gene activation when enhancers were active or silent, they could identify the target genes of previously...efficient. In general, epigenetic chromatin modifications are correlated with alterations in gene expression...updated Jun 24, 2020. Epigenetic modifications are an additional layer of control over gene expression that ...proteins that help package DNA, were associated with gene expression or silencing. To alter DNA modifications...oxidation and repair factors that allow for increased gene activation at the targeted site compared to other...the only CRISPR-based technology designed to alter gene expression. Fusing dCas9 to a transcriptional activator...a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers. Nature Biotechnology...

  20. Plasmids for Stem Cell Research

    Type
    Collection
    ...table based on plasmid name, species, gene name, and more. Plasmid Gene/Insert Vector Type PI Publication ...cell lines with endogenously-tagged gene variants or browse Addgene’s entire collection of Stem Cell Research...against p53 in different gene/insert combinations A more efficient method to generate integration-free human...non-integrating plasmid from blood cells with unique epigenetic and gene expression signatures. Cell Res. 2011 Mar...human cells to pluripotency by activating enogenous genes Human pluripotent reprogramming with CRISPR activators...S1934-5909(19)30423-0. Schöler You can also find gene-specific plasmids at the following links: NANOG ...Browse Addgene's collection of plasmids for the generation and study of stem cells, from iPSC generation...
Showing: 301 - 320 of 1144 results