We narrowed to 152 results for: Gene

...excised, and gene expression proceeds. Gene Knockout: Placing the loxP sites on either side of a gene (called...shRNA expression. Gene Switch: These constructs contain two genes of interest, Genes A and B. When Cre... few popular recombinase-dependent gene expression strategies. Gene Expression: A stop codon flanked with...LSL" cassette) placed upstream of a gene of interest will prevent gene expression in the absence of Cre.... loxP"), will permit gene expression until Cre is present, at which time the gene will be disrupted or...Cre is absent, only Gene A is translated correctly. Cre expression excises Gene A and alters the reading...backbone to regulate your gene of interest. Depending on the construct, gene expression may be activated...

...Additional Gene Silencing Resources A modular plasmid assembly kit for multigene expression, gene silencing...plant-optimized plasmids for cloning, gene expression, reporter assays, genome editing, gene silencing, and more. Plasmid... a new window) — Tools for gene and protein expression and mapping of gene expression in plants. Grassius...Resources Cloning & SynBio Genome Editing Gene Silencing Reporters Addgene’s repository contains many plasmid ...from genome engineering and gene expression to reporter systems. Addgene’s collection includes plasmids...plasmids containing promoters that drive general and species-specific gene expression. Cloning and Synthetic ...most popular plasmids for gene editing in plants. Please refer to Addgene's Plant CRISPR Resources for...

...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Base Edit ID Plasmid Gene/Insert...Plasmid Gene/Insert Selectable Marker PI Publication Return to top Interfere ID Plasmid Gene/Insert Promoter...Plasmid Gene/Insert Promoter Selectable Marker Publication Return to top Prime Edit ID Plasmid Gene/Insert...Plasmid Gene/Insert Selectable Marker PI Publication Return to top RNA Knockdown ID Plasmid Gene/Insert...ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Epigenetics ID Plasmid ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top CRISPR Resources Addgene has ...Marker PI Publication Return to top Purify ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication...

... Depositor Collections Gene Vector Core Viral Vectors Gene Vector Core Viral...remove genes flanked by either loxP or FRT sites respectively, and empty vectors to express your gene of ...and puromycin resistance gene from the CMV promoter pCDH-CMV 72265 Express gene of interest from the CMV...pCDH-EF1 72266 Express gene of interest from the EF-1 promoter pCDH-CB 72267 Express gene of interest from ...pCDH-CB-IRES-copGFP-T2A-Puro 72299 Express gene of interest from the CB promoter pCDH-EF1s 72484 Express gene of interest from ...recombinases. Genes can be cloned into these transfer vectors using shuttle vectors pICPIS-EF1 (Addgene #73355...College of Medicine Gene Vector Core Depositor...

...Plasmids by Gene The following table lists genes in the ARF GTPase family, information on each gene, and examples...each gene. Click the gene name for a full list of plasmids available. Please note that some genes may ...Plasmids by Gene The following table lists genes in the ARF GAP and GEF family, information on each gene, and...have plasmids available for request. Family Gene Entrez Gene ID Length (aa) Example Expression Systems ...Browse Addgene's collection of ARF family plasmids. ARF family members include regulatory GTPases, GEFs...

...Screening Use pooled CRISPR libraries to screen for genes involved in specific biological processes. For more... Plasmid Pooled Libraries . ID Name Description Genes/Insert Selection PI Human knockout pooled libraries...library in backbone lentiGuide-Puro targeting 19,114 genes and containing 76,441 unique sgRNAs along with 1000...SpCas9. 76,441 unique sgRNAs targeting 19,114 human genes along with 1000 non-targeting controls Puromycin...library in backbone lentiCRISPRv2 targeting 19,114 genes and containing 76,441 unique sgRNAs along with 1000...and 76,441 unique sgRNAs targeting 19,114 human genes along with 1000 non-targeting controls Puromycin... in backbone XPR_502 (P65 HSF) targeting 18,885 genes and containing 56,762 unique sgRNAs. This backbone...

...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert...Drosophila ID Plasmid Gene/Insert Promoter PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter...elegans ID Plasmid Gene/Insert Promoter PI Publication Return to top Yeast ID Plasmid Gene/Insert Promoter...CRISPR plasmids for activating or upregulating gene expression in various model organisms. CRISPR...peptide can increase transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator...activator to promoter or regulatory regions of your gene of interest. If the plasmid that you choose does... PI Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...

... vector for gene expression pINDUCER21 (ORF-EG) - Inducible lentiviral gene expression ...destination vector for gene expression Lentiviral backbones made by Addgene Addgene has an extensive collection...host cell and are designed for a user to insert a gene of interest. They may contain tags or fusion proteins...These vectors allow you to test the function of your gene of interest in a controlled environment under various...we will assume that you want to express a gene; however, we have included a function section for if you...If you want to drive expression of your favorite gene, you will need a plasmid with a promoter that...mTagBFP2 tag pNIC-GST-TEV-GG - Cloning of target gene with N-terminal GST tag and TEV cleavage Transient...

...promoter upstream of your gene of interest. If your gene of interest should be generally active and only turned...Tet-On Systems for Doxycycline-inducible Gene Expression . Curr Gene Ther, 16 (3), 156–167. https://doi.org...Tet-On system for regulated gene expression through viral evolution . Gene Ther, 13 (19), 1382–1390. https... methods to induce gene expression in experimental systems. 2nd and 3rd Generation Systems Subsequent ...Learn about tetracycline-regulated gene expression and find plasmids for your experiments using tet- ...repressible promoters are powerful tools to control gene expression and study biological targets. The tetracycline... for rapid, efficient, and reversible control of gene expression in eukaryotes. To avoid secondary effects...

...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Yeast ID Plasmid Gene/Insert...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Xenopus ID Plasmid Gene/Insert...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top CRISPR Resources Addgene has ...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...Synthetic Biology Networks and Gene Regulation Synthetic Biology: Networks and Gene Regulation SynBio Resources...Addgene's collection of synthetic biology plasmids related to Networks and Gene Regulation Plasmid...Metabolism Networks & Gene Regulation Sensing & Signaling Strains Browse Addgene's collection of synthetic...regulatory elements, pre-assembled genetic circuits, and higher level gene networks. Examples Include: promoters...repressors and activators logic gates Networks and Gene Regulation Plasmids Search the table by keyword ...plasmids available from this depositor's lab. Plasmid Gene/Insert Vector Type Promoter PI Publication Back ...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Yeast ID Plasmid Gene/Insert...plasmids for transcriptional repression of target genes. CRISPR...transcriptional repressor peptide like KRAB, can knock down gene expression by interfering with transcription. This...interference is most efficient when the gRNA targets your gene of interest’s promoter, enhancer, or the beginning... PI Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...zebrafish gene of interest? Search Addgene's collection for plasmids that contain a zebrafish gene or sequence...zebrafish-specific gene expression database that provides information about when genes are active in developing...developing embryos. zfRegeneration - A database for gene expression profiling during regeneration. Seurat - R ...-type specific conditional gene inactivation - Jeffrey Essner Lab GeneWeld Vectors for Targeted Integration... finger nucleases, TALENS, and CRISPR/Cas9-based gene editing make the zebrafish a stalwart research model... model. With more than 70% of human disease genes having an ortholog in zebrafish, this species has more...kits are described below . ID Plasmid Description Gene/Insert PI Return to top Highlights of Zebrafish ...

...essential tool for studying gene function, modeling diseases, and advancing gene therapy development. In ...containing adenoviral or herpesviral helper genes. These genes are not present in the AAV genome, but are...helper genes normally contained in the helper plasmid. Packaging Plasmids Available with Addgene's Viral... (Rep/Cap plasmids) in Addgene’s repository that express AAV Rep and Cap genes for packaging in specific... lines. Helper plasmids generally encode the adenoviral E4, E2a, and VA genes. This table contains helper...containing the AAV replication (Rep) and capsid (Cap) genes necessary for the production and assembly of viral...search or sort this table based on plasmid name, gene name, in-stock viral prep availability, viral packaging...

...briggsae , etc.) gene or sequence: ID Plasmid Gene/Insert PI Don’t see your gene listed? Know of some great...can knock down genes by feeding worms bacteria expressing dsRNA or use CRISPR/Cas9 gene editing to easily... used in worms, search Addgene's entire collection. ID Plasmid Description Gene/Insert PI Plasmids Expressing...which diminishes epigenetic silencing in the germline. A "FLP-Out" system for controlled gene expression in...Expressing Worm Genes/Inserts The table below lists plasmids that contain a worm ( C. elegans, C. briggsae...Plasmids for the temporal and spatial control of gene expression in C. elegans by combining expression...Editing Efficiency Fluorescent Tagging of Endogenous Genes with SapTrap Even more elegant: Single injection...

...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Base Edit ID Plasmid Gene/Insert... Plasmid Gene/Insert Selectable Marker PI Publication Return to top Interfere ID Plasmid Gene/Insert Promoter...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Prime Edit ID Plasmid Gene/Insert...in plants. To find emtpy gRNA plasmids, visit Addgene's Empty gRNA Expression Vectors page and search ... PI Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Base Edit ID Plasmid Gene/Insert...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Interfere ID Plasmid Gene/Insert...Description Gene/Insert Promoter Selectable Marker PI Publication Return to top Purify ID Plasmid Gene/Insert... in yeast. To find emtpy gRNA plasmids, visit Addgene's Empty gRNA Expression Vectors page and search ... PI Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...type or mutant ORFs, gene fragments, and gRNAs for genes linked to neurodegenerative disorders. Find plasmids...within the huntingtin (HTT) gene. An individual with the mutated HTT gene will have huntingtin proteins...association between a gene and late-onset AD. It is more likely a combination of gene variation and lifestyle...Localization , Optogenetics Plasmids , and Chemogenetics Plasmids . Find plasmids for endogenous gene tagging... are associated with known genetic mutations in one of the following genes: SOD1, C9ORF72, TARDBP, FUS...these additional genes may play in Alzheimer's disease. Research Tools Neurodegeneration Plasmid Collection...heritable gene silencing with CRISPRoff-v2.1 . (Nuñez et al. Cell. 2021) Conduct multimodal genetic screens...

...targeted to various zebrafish genes. The table below lists zebrafish genes and associated pairs of zinc...of zebrafish genes and associated pairs of zinc finger arrays that can be used to generate ZFNs targeted...arrays that can be used to generate ZFNs targeted to a site within that gene. To construct ZFN expression...targeted to a site within that gene. Plasmid...Engineered Zinc Finger Arrays for Endogenous Zebrafish Gene Targets Recent published reports have shown that...tested for activity as ZFNs in zebrafish. Target Gene Name Zinc Finger Arrays Engineering Method Full ...aCCTCGCCTCagtgtGACGGAGGAc Swachmann-Bodian-Diamond Syndrome Gene (sbds) OZ545 and OZ546 OPEN OPEN tGTTGCCGTCgtgagGATGAAGAAa...

...Cut ID Plasmid Gene/Insert Promoter PI Publication Return to top Interfere ID Plasmid Gene/Insert Promoter...Nick ID Plasmid Gene/Insert Promoter PI Publication Return to top Prime Edit ID Plasmid Gene/Insert Promoter...search the table for "Bacteria". Activate ID Plasmid Gene/Insert Promoter PI Publication Return to top Base...Promoter PI Publication Return to top Purify ID Plasmid Gene/Insert Promoter PI Publication 64325 3xFLAG-dCas9... bacteria. To find emtpy gRNA plasmids, visit Addgene's Empty gRNA Expression Vectors page and search ... PI Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...