We narrowed to 365 results for: Pol;

1. How to Be an Excellent Trainee

   Type

   Blog Post

   ...It’s often easiest to train individuals who are polite, appreciative, and clear about their needs. Remember...

2. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

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   ... the cells could be batch-sorted to isolate a polyclonal cell line with strong gas vesicle expression ...

3. Plasmids 101: Stringent Regulation of Replication

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   Blog Post

   ... from the ori. In the elongation phase, a DNA polymerase synthesizes a new DNA strand at each replication...

4. Plasmids 101: Broad Host Range Plasmids

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   ...structural elements, oris interact with various RNA polymerases and proteins from bacterial hosts. In a plasmid...

5. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

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   ... Marc Sze, Victoria McGovern, and Julie Wolf. Apologies to anyone I’ve inadvertently left off this list...

6. Transferable Skills Guide: External Collaborations

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   ...skill is a hallmark for jobs focusing on: Science Policy, Business Development, Administration and Intellectual...

7. AAV Titers: Where Do They Come From And What Do They Mean?

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   ...Paolo, et al. "Systemic errors in quantitative polymerase chain reaction titration of self-complementary...

8. Career Insights: Technical Support Specialist

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   Blog Post

   ...the job. When dealing with customers, you must be polite, friendly, patient, attentive, empathetic, and ...

9. The Challenges of Cell Culture

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   ...whether the cell lines had isoenzymes and genetic polymorphisms specific to their origins. This technique is...

10. Plasmids 101: E. coli Strains for Protein Expression

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    ...induction system. The DE3 lysogen expresses T7 RNA polymerase (RNAP) from the bacterial genome under control...

11. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

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    ...mitochondrial matrix (Rhee et al., 2013) and determine the topology of the mitochondrial calcium uniporter (Martell...

12. Harnessing Bacterial Toxins for Allelic Exchange

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    ...tuner: a computer program for the construction of polylinker sequences of molecular vectors". Proceedings ...

13. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

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    ...Bendezú, Felipe O., et al. "Spontaneous Cdc42 polarization independent of GDI-mediated extraction and actin-based...

14. Antibodies 101: Introduction to Immunofluorescence

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    Blog Post

    ...common is that secondary antibodies are generally polyclonal, which means that multiple secondary molecules...

15. Antibodies 101: Single Chain Fragment Variables (scFvs)

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    ...recombinant antibody. They are ~25 kDa single polypeptides that contain the variable light chain (VL) and...

16. Adeno-associated Viruses (AAVs) for Genome Editing

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    ...choice. These vectors are very similar, both with polylinkers for the addition of gene-specific 1 kb homology...

17. Multiple Plasmids at a Low Price: Kits from Addgene

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    Blog Post

    ...terminators can be used to precisely tune your polycistronic E. coli expression constructs. Some applications...

18. Using Phosphoserine to Study Protein Phosphorylation

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    Blog Post

    ...extension and translation of the pSer-incorporated polypeptide. These three components were shown to effectively...

19. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

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    Blog Post

    ...Addgene. Kaminski R, Chen Y, Fischer T, Tedaldi E, Napoli A, Zhang Y, Karn J, Hu W, Khalili K. Elimination...

20. CRISPR 101: Epigenetics and Editing the Epigenome

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    ...https://doi.org/10.1093/nar/gky1286 Hilton IB, D’Ippolito AM, Vockley CM, Thakore PI, Crawford GE, Reddy...