Skip to main content
Addgene
Showing: 301 - 320 of 559 results

  2. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...been used for protein expression. Plant systems share many of the same features and processing requirements...loves being able to help the scientific community share plasmids.   Additional Resources Thermofisher Protein...

  5. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...that matures quickly and the other slowly, see Verkhusha et al.), one benefit of the common FP timers is...19889833. PubMed Central PMCID: PMC2801723. 3. Verkhusha, Vladislav V., et al. "An enhanced mutant of red...

  7. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    ...designing the best donor DNA, and avoiding common mishaps in this class of genome edit. Knock-in mutations...2020). 10.1016/j.jbiotec.2019.11.010 Wright, D. W., Shah, S. S., Heyer, W. D. Homologous recombination and...

  8. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ... 1996, is an eleven β-sheet-containing “barrel” shape, with the chromophore concealed at the center of...Gal Haimovich of greenfluorescentblog. Gal will share his list of 10 things to keep in mind when choosing...

  9. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...visualize numerous molecules or structures at once (Shaner, 2007). In contrast to epitope tags, FPs are bigger...2009.12 (2009): pdb-top63. PubMed PMID: 20150100. 4. Shaner, Nathan C., George H. Patterson, and Michael W....

  11. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...frequency in a reference of highly expressed genes (Sharp and Li, 1987). In 2000 the Ikemura lab created the...Genet 12:683–691. https://doi.org/10.1038/nrg3051 Sharp PM, Li W-H (1987) The codon adaptation index-a measure...

  15. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ...make a career in academia, you are likely in good shape, as your own adviser will probably have a lot of...webpages. Importantly, find a good mentor that can share relevant experience and resources with you to ease...

  16. How to Be an Excellent Trainee

    Type
    Blog Post
    ...protocol steps and other tips and tricks your mentor shares. Additionally, go ahead and write down where that...that one drug is stored or how to access the shared server. The blue box labeled “A5” on the second rack...

  17. How to Write a Scientific Review Article

    Type
    Blog Post
    ...at the limited number of hits. So, here, I will share my process with you. But before I do that, let me...formatting guidelines, etc.) you have some criteria to shape the document. Get and use a reference management...

  19. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ...you are making your hypothesis stronger. When you share data at a conference poster session, you don’t necessarily...  Communication (or lack thereof): Information sharing gets more challenging the bigger an organization...

  20. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...tissue morphogenesis, organ development, and the shaping of the extracellular matrix. During immune responses...cellular processes.   Figure 1: Integrins can shape into three conformations: (A) bent-closed, (B) extended-closed...
Showing: 301 - 320 of 559 results