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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 661 results for: SIM;

Showing: 301 - 320 of 661 results

  1. Components of CRISPR/Cas9

    Type
    Blog Post
    ...using CRISPR/Cas9 for genome editing, researchers simply need to express a gRNA designed to direct Cas9 ...these arrays, they did not know their functions and simply called the repeated elements “direct repeats” and...

  3. Advice for Scientists Starting a Lab

    Type
    Blog Post
    ...strong collaborative research team motivated by similar visions and goals? We continue our conversation...and a friend. George Church runs his lab fairly similarly to his advisor Wally Gilbert: “Very interdisciplinary...

  4. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...can either express the plasmid DNA transiently (similar to bacteria) or incorporate the genetic material.... Positive selection in mammalian cells works similarly to that in bacteria and a table of the most commonly...

  5. OCaMP: A New Calcium Indicator for Neural Imaging

    Type
    Blog Post
    ..., where multiple fluorescent tools are used simultaneously to monitor or control different cell types ...4.0 International license. Figure 5: A) Simultaneous fluorescence (upper trace) and electrophysiological...

  6. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ...Packaging using 293T cells This method is very similar to lentiviral packaging methods. The three components...constructs. Why do many γ-retroviral plasmids have similar names? The nomenclature of the plasmid backbone...

  7. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ... an advanced search option. For the former, you simply type in identifier keywords (Cas name, RefSeq ID...phylogenetic nomenclature and discover orthologs with similar properties (Makarova et al). CasPEDIA is an evolving...

  8. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ...organisms. This is often cDNA, shRNA, or something similar, and is contained on the transfer plasmid. If expression...different from gamma-retrovirus, but they perform very similar functions to infiltrate the host cell and deposit...

  12. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    ...management, and how does it relate to science? In the simplest of terms, a software product manager helps ensure...could code the best operations software possible. Similarly, when the developers needed to know information...

  13. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...this post in the graphic below - including some simple comparisons. The easy multiplexing afforded by...integrated RNA and CRISPR/Cas toolkit in human cells. Nissim L, Perli SD, Fridkin A, Perez-Pinera P, Lu TK. ...

  15. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    ...getting too nervous or having a memory lapse, I simply pause and take a deep breath before the panic sets...make a strategy that can help. Even something as simple as telling yourself, “I can stop and check my notes...

  16. All in a Twist: dsRNA

    Type
    Blog Post
    ...been clinically exploited for antiviral purposes similar to that of vaccines. If you know the sequence of...responses can mount in the absence of infection. Similarly, if endogenously synthesized dsRNA finds its way...

  17. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ...successfully used in conditions that are the most similar to yours. Take a look at the publications cited...application, a publication successfully used it in a similar way as you plan to. To secondary or not to secondary...

  18. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...conjugated in the lab. In the indirect approach, you can simply keep a stock of conjugated secondary antibodies...regardless of the number of protein targets, simplifying multiplex assays.  A brief note Since the indirect...

  19. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    ...Fandino, L. B., Labasky, M. E., Forostyan, T., Colosimo, L. K., Graham, T. E. (2015). Rapid parallel measurements...Fluorescent Protein Oligomerization Seeing Red: Simple GFP Photoconversion Light Sheet Fluorescence Microscopy...
Showing: 301 - 320 of 661 results