We narrowed to 665 results for: SIM;

...prime editing, achieving up to 3-loci MPE with similar high efficiencies, which is scalable for a higher...adeno-associated virus (AAV) and lentivirus, demonstrating simultaneous editing of genes against multiple genetic diseases...

...sequencing instruments by enhancing the efficiency and simplicity of library prep. As part of our mission, we’ve...here is that, as more samples are sequenced simultaneously, it gets harder and harder to obtain balanced...

...pocket.   5. +AMINO- by iGreenEarth AMINO is a very simple app that packs important biological information... names have a B, J, X, or Z. Some alternative, similar apps: Findings Lab Notebook Cell Culture Split...

...using CRISPR/Cas9 for genome editing, researchers simply need to express a gRNA designed to direct Cas9 ...these arrays, they did not know their functions and simply called the repeated elements “direct repeats” and...

...BamHI) are capable of cleaving sequences which are similar, but not identical, to their defined recognition... the DNA mass of comparably intense samples of similar size. Run the gel at 80-150V until you have good...

...strong collaborative research team motivated by similar visions and goals? We continue our conversation...and a friend. George Church runs his lab fairly similarly to his advisor Wally Gilbert: “Very interdisciplinary...

...can either express the plasmid DNA transiently (similar to bacteria) or incorporate the genetic material.... Positive selection in mammalian cells works similarly to that in bacteria and a table of the most commonly...

...Packaging using 293T cells This method is very similar to lentiviral packaging methods. The three components...constructs. Why do many γ-retroviral plasmids have similar names? The nomenclature of the plasmid backbone...

...not robust. Sampling noise There is, of course, simple noise in the process of sampling and inference-...are known. The author submits the research plan (similar to a preregistration, but usually with a fully ...

...research purposes requires stable host cells (the simplest example would be a transformed mesophilic, for... warm weather. Psychrophilic enzymes face very similar issues when forced to express in mesophilic host...

...NG (Nishimasu et al., 2018). The David Liu Lab similarly described xCas9, which displayed the ability to... (HypaCas9). This nuclease showed decreased or similar off-target activity, relative to eSpCas9 and SpCas9...

...management, and how does it relate to science? In the simplest of terms, a software product manager helps ensure...could code the best operations software possible. Similarly, when the developers needed to know information...

..., where multiple fluorescent tools are used simultaneously to monitor or control different cell types ...4.0 International license. Figure 5: A) Simultaneous fluorescence (upper trace) and electrophysiological...

... an advanced search option. For the former, you simply type in identifier keywords (Cas name, RefSeq ID...phylogenetic nomenclature and discover orthologs with similar properties (Makarova et al). CasPEDIA is an evolving...

...organisms. This is often cDNA, shRNA, or something similar, and is contained on the transfer plasmid. If expression...different from gamma-retrovirus, but they perform very similar functions to infiltrate the host cell and deposit...

...this post in the graphic below - including some simple comparisons. The easy multiplexing afforded by...integrated RNA and CRISPR/Cas toolkit in human cells. Nissim L, Perli SD, Fridkin A, Perez-Pinera P, Lu TK. ...

...and mentees. Because the participants are at a similar development stage, they have a lot to learn from...Sometimes it is helpful to find peers that share a similar direction or problem. For example, a group of people...

...inhibition, with TRIM-away targeting of mTOR producing similar results to rapamycin inhibition. To assess TRIM21...control will enable future researchers to better simulate physiological gene expression regimes that cannot...

...successfully used in conditions that are the most similar to yours. Take a look at the publications cited...application, a publication successfully used it in a similar way as you plan to. To secondary or not to secondary...

...conjugated in the lab. In the indirect approach, you can simply keep a stock of conjugated secondary antibodies...regardless of the number of protein targets, simplifying multiplex assays.  A brief note Since the indirect...