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We narrowed to 732 results for: SON

Showing: 301 - 320 of 732 results

  1. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    ...adolescence passed on a heritable trait to their grandsons that decreased their likelihood of death from ... Ong-Abdullah, Meilina, et al. "Loss of Karma transposon methylation underlies the mantled somaclonal .... 3. Kaati, Gunnar, Lars O. Bygren, and Soren Edvinsson. "Cardiovascular and diabetes mortality determined...

  2. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    ...slightly if you know a bit more about the other person. If you have a poster or talk at the conference...there parts of your elevator pitch where the other person seemed confused, bored, or particularly excited...after. For one conference I went to, I made it a personal goal to ask one question each day (this may be...

  3. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...., Polali, S., Anderson, E. D., Bell, A. M., Tzouanas, C. N., Avants, B. W., & Robinson, J. T. (2019).....4124.  Kattnig, D. R., Evans, E. W., Déjean, V., Dodson, C. A., Wallace, M. I., Mackenzie, S. R., Timmel...

  4. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...recommend using a Tris-based lysis buffer and sonication.  The obtained virions from cells and supernatant....2020.120 Lock, M., Alvira, M. R., Chen, S.-J., & Wilson, J. M. (2014). Absolute determination of single-stranded...L. H., Xiao, R., Lock, M., Lin, J., Korn, M., & Wilson, J. M. (2010). Efficient serotype-dependent release...

  5. Pooled Library Amplifications

    Type
    Blog Post
    ...efficiency. Figure used with permission from the Mark Dawson lab Re-transformation of the SPLINTR Libraries ...recombined backbones that may replicate faster. For this reason, if your library has inserts of different sizes... Hannah Dotson...

  6. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    ...is very tricky to use and fussy to optimize. One reason could be the instability of the supposedly alien...many may find this inconvenient. Another possible reason for the trickiness of NgAgo is the inability of... not snipping DNA, he tries random things (101 lessons on Spanish, C++, guitar, photography) and often...

  7. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ...next thing. If I made a mistake, I evaluated what lessons I had learned – call it “troubleshooting.” Trying...I asked some colleagues to share valuable life lessons they have learned during their scientific training... them in a blog post on Born to Science: Life Lessons from Scientific Training More and more voices ...

  8. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...Praparaten. Leipzig: S. Hierzel.
 J.A Dent, A.G. Polson, M.W. Klymkowsky. (1989) Development, 105.61-74... Ross A, J. Hecksher-Sorensen, R. Baldock,  D. Davidson. (2002) Science, 296, 541-545. Pubmed. A. Ertürk.... Kim, S. Kalyanasundaram, A.S. Andalman, T.J. Davidson et al. (2013) Nature, 497, 332-337. Pubmed. R....

  9. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    ...the statistical independence assumption of the Poisson process. This modification also eases the interpretation...orders.  Our model factors the likelihood in our Poisson process into two components: 𝜃x(t) and 𝜓z(t)....contains an intractable integral of the product of a Poisson and multivariate log-normal likelihoods. Effects...

  10. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...qPCR runs before a new one is needed. For these reasons, we are in the process of transitioning our titering...for the detection of low copy numbers. For this reason, it can be used in the detection of rare sequences...PMID: 24328707. PubMed Central PMCID: PMC3991984. Song, Neng, et al. "Detection of circulating Mycobacterium...

  11. An Introduction to Adenovirus

    Type
    Blog Post
    ...genes that apparently contribute to virulence (Tollefson et al., 1996) – cell death has been reported when...6301263. Servellita, V., Sotomayor Gonzalez, A., Lamson, D. M., Foresythe, A., Huh, H. J., Bazinet, A. ...10.1182/BLOOD-2007-02-062117. PMID: 17510320. Tollefson, A. E., Scaria, A., Hermiston, T. W., Ryerse, ...

  12. Tips for arabidopsis transformation

    Type
    Blog Post
    ...Arabidopsis is a fantastic model organism for many reasons, not the least of which is ease of transformation...a protocol for antibiotic selection, check out Harrison et al., 2006. Tips: Don’t plate seeds too densely... 24032037. PubMed Central PMCID: PMC3759027. 2 Harrison, S. J. et al. (2006) ‘A rapid and robust method...

  13. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...gross comparison of AAV.PHP.eB and AAV.PHP.S to their parental vectors. To quantify this comparison, capsids...PubMed PMID: 28671695. 2. Deverman BE, Pravdo PL, Simpson BP, et al. Cre-dependent selection yields AAV variants...

  14. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ...screening! This blog was written by Addgenie Hannah Dotson.  Resources and references References Genome-...protocols.  References McDade JR, Waxmonsky NC, Swanson LE, Fan M. Practical Considerations for Using Pooled...  Hannah Dotson...

  15. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    ...meeting who felt isolated at a conference for any reason was a Singleton. Figure 1: The Binning Singletons...Gorski, Mark O. Martin, Maureen Berg, Michael D. L. Johnson, Tiffany Lowe-Power, Eric Griffis, Janet Iwasa,...Dunivin, Jen Greenwich, Susanna Harris, Heather Hendrickson, Jonathan Jacobs, Mo Kaze, Christina Kellogg,...

  16. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type
    Blog Post
    ...vertebrate embryogenesis. To illustrate some of the reasons that zebrafish have been embraced as a model organism...examine the consequences of manipulating genes. One reason for the success of zebrafish as a model organism... 2016). One common and robust strategy uses a transposon called Tol2 to randomly integrate foreign DNA...

  17. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...Fuller CK, Cameron P, Fazzio TG, Zhu LJ, Wolfe SA, Sontheimer EJ (2018) NmeCas9 is an intrinsically high-fidelity.... Kim E, Koo T, Park SW, Kim D, Kim K, Cho H-Y, Song DW, Lee KJ, Jung MH, Kim S, Kim JH, Kim JH, Kim ...Topkar VV, Nguyen NT, Zheng Z, Gonzales APW, Li Z, Peterson RT, Yeh J-RJ, Aryee MJ, Joung JK (2015) Engineered...

  18. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...mammalian cell lines. However, in those same comparisons, the AbuGoot lab reported no detectable effect...and can be easily programmed for new genes. In comparison with other large insert techniques like HITI ... Rachel Leeson...

  19. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ... can arise, and has arisen, for many different reasons. Exposure to antibacterial drugs provides a selection...households by observing proper cleanliness and personal hygiene standards. Handwashing is an essential...spread of antibiotic resistant bacteria from one person to the next. For more information and tips on preventing...

  20. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...lack of documentation and high turn-over of lab personnel. If you are unsure of the background of the lines... such as serum, other cell lines, or infected personnel and can persist undetected; unlike infections ..., Kassem H.S., Pepper S.D., Hey Y., Ward T.H., Margison G.P. “Mycoplasma infection significantly alters...
Showing: 301 - 320 of 732 results