We narrowed to 421 results for: SUA

1. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

   Type

   Blog Post

   ... genome editing, activating gene expression, visualizing genomic loci, and much more. Now, researchers...

2. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

   Type

   Blog Post

   ...some sort of model (cartoons are appreciated) to visually sum up all of the conclusions. In any case, the...

3. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

   Type

   Blog Post

   ...how the output changes as a function of input. Usually that function is in the shape of an S; there is...

4. Career Coaching for Scientists: Why and Where Do I Find One?

   Type

   Blog Post

   ...this is a style that will help you. A good coach usually will be a clear communicator and make direct suggestions...

5. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

   Type

   Blog Post

   ...the Addgene Blog Learn about techniques for visualizing protein turnover Learn about plasmids for in ...

6. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

   Type

   Blog Post

   ...sites. Knock-in experiments are important for visualizing ncRNA localization and function, but directly...

7. Viral Vectors 101: Calcium Sensors

   Type

   Blog Post

   ...would like to monitor? Neurons, for example, have unusually fast calcium dynamics, whereas non-neuronal systems...

8. Viral Vectors 101: Virus Safety

   Type

   Blog Post

   ...19q13.4 qtr. (AAVS1)). For these reasons, AAV is usually classified as a BSL-1 safety level. Adenovirus ...

9. Hot Plasmids - October 2020

   Type

   Blog Post

   ... lab has developed AAV vectors to selectively visualize excitatory and inhibitory synapses in the brain...

10. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

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    Blog Post

    ...Vogelstein showed, for the first time, there were unusual patterns of DNA methylation detected in a human...

11. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type

    Blog Post

    ... one’s figures, or at least the majority of the visual data. This central box is then flanked by two equally-sized...

12. A Look at Addgene's QC Process

    Type

    Blog Post

    ...these resources allow QC scientists to easily visualize and identify sequence differences between our ...

13. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type

    Blog Post

    ...spreadsheet to complete Step 1. Steps 2 and 3 are usually very easy — our tech transfer team will communicate...

14. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type

    Blog Post

    ...non-homologous endjoining (NHEJ). Cas9-mediated NHEJ usually destroys the PAM site due its proximity to the ...

15. Plasmids 101: Terminators and PolyA signals

    Type

    Blog Post

    ... transcript. Rho-dependent terminators are not usually employed in plasmid-based expression systems, so...

16. Gaining Leadership Skills Volunteering at a Professional Organization

    Type

    Blog Post

    ...Volunteering on these committees was real work; meetings usually resulted in actionable tasks and I made a point...

17. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type

    Blog Post

    ...difficult people: Hostile-aggressives, who are usually antagonistic and impolite, Complainers, who spend...

18. Antibody Guide

    Type

    Guide

    ...Chromatin immunoprecipitation (ChIP) Visualization - used to visualize protein location. Includes: Immunofluorescence...immunoprecipitation. Antibody Applications - Visualization Methods Visualization methods such as immunofluorescence...individual assay conditions. Controls for visualization methods Visualization methods should employ positive, negative...Specificity Other Affinity Reagents Handling Visualization Signal Amplification Application Overview Quantification...Quantification Methods Capture Methods Visualization Methods Cell Sorting Methods Multiplex Assays Selection...accessible to antibodies. Detection of sdAbs is usually achieved by conjugation of a signaling molecule...before conjugating your antibodies. Antibody Visualization The ability of an antibody to specifically bind...

19. 3D Printing Meets CRISPR Cas9

    Type

    Blog Post

    ...Learn All about CRISPR Use CRISPR for Genome Visualization Tips for a First Time CRISPR User by a First...

20. Production of Virus in Insect Versus Mammalian Cells

    Type

    Blog Post

    ...production when roughly 60–70% of cells have died, usually after four to five days. This means that viral ...