Plasmid Tools for Microbiome Studies
Type
Blog Post
...can be used to introduce fluorescent markers to visualize localization and distribution of gut bacteria ...
We narrowed to 424 results for: SUA
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Viral Vectors 101: An Introduction to AAV
TypeBlog Post...ballpark estimation. Once expressed, viral cargo is usually quite stable and in some mammalian tissues has ... -
Transferable Skills Guide: Public Speaking
TypeBlog Post...public speaking isn't a performance art. You’re (usually) presenting either to share knowledge or to invite... -
Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics
TypeBlog Post... of a light switch. Neurons are not typically persuaded to fire when light is shined on them, but the ... -
Addgene's Tips for Plasmid Quality Control
TypeBlog Post...to perform alignments of multiple sequences to visually check for homology and mismatches. Do you have... -
Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9
TypeBlog Post... genome editing, activating gene expression, visualizing genomic loci, and much more. Now, researchers... -
Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing
TypeBlog Post...some sort of model (cartoons are appreciated) to visually sum up all of the conclusions. In any case, the... -
CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt
TypeBlog Post...how the output changes as a function of input. Usually that function is in the shape of an S; there is... -
Career Coaching for Scientists: Why and Where Do I Find One?
TypeBlog Post...this is a style that will help you. A good coach usually will be a clear communicator and make direct suggestions... -
A Look at Addgene's QC Process
TypeBlog Post...these resources allow QC scientists to easily visualize and identify sequence differences between our ... -
CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9
TypeBlog Post...sites. Knock-in experiments are important for visualizing ncRNA localization and function, but directly... -
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation
TypeBlog Post...Vogelstein showed, for the first time, there were unusual patterns of DNA methylation detected in a human... -
Cpf1 Update: Comparison to Cas9 and NgAgo
TypeBlog Post...non-homologous endjoining (NHEJ). Cas9-mediated NHEJ usually destroys the PAM site due its proximity to the ... -
Plasmids 101: Terminators and PolyA signals
TypeBlog Post... transcript. Rho-dependent terminators are not usually employed in plasmid-based expression systems, so... -
Split-BioID: An Improved Method for Studying Protein-Protein Interactions
TypeBlog Post...the Addgene Blog Learn about techniques for visualizing protein turnover Learn about plasmids for in ... -
A Guide to Designing a Scientific Poster: Content, Layout, and More
TypeBlog Post... one’s figures, or at least the majority of the visual data. This central box is then flanked by two equally-sized... -
Quickest Way to Deposit Plasmids: The Deposit Spreadsheet
TypeBlog Post...spreadsheet to complete Step 1. Steps 2 and 3 are usually very easy — our tech transfer team will communicate... -
Viral Vectors 101: Calcium Sensors
TypeBlog Post...would like to monitor? Neurons, for example, have unusually fast calcium dynamics, whereas non-neuronal systems... -
Viral Vectors 101: Virus Safety
TypeBlog Post...19q13.4 qtr. (AAVS1)). For these reasons, AAV is usually classified as a BSL-1 safety level. Adenovirus ... -
Hot Plasmids - October 2020
TypeBlog Post... lab has developed AAV vectors to selectively visualize excitatory and inhibitory synapses in the brain...