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  2. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ... be included in a single PCR, for example for subcloning genes from different templates. All PCRs are ...can be included at the 5’ end of either primer. Subcloning - To move a gene from one vector to another simply...

  3. Plasmids 101: Colony PCR

    Type
    Blog Post
    ... the last step is to mini-prep these clones and submit the plasmids for Sanger sequencing. Sequencing ...there aren’t mutations in your insert. Make sure to submit multiple positive clones for sequencing to verify...

  4. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    ...range from coordinating the grant application and submitting it, to evaluating the capacity of an organization...deadlines, and we ensure that grant reports are submitted and project evaluations are completed. To enter...

  7. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...interchangeable. While these differences may seem subtle they result in a key physiological difference between...useful in gene therapy applications where a distinct subset of the cellular population needs to be targeted...

  9. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...transcription to convert amplified DNA to RNA for subsequent detection by Cas13. This amplification step in...trans-ssDNA, including the ssDNA-FQ reporter are subsequently degraded at a rate of ~1,250 cuts per second...

  10. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ... activating chemogentic receptor (hM3Dq) into a subset of neurons within the LC of mice. When those neurons...further and showed that the activity of a particular subset of excitatory neurons (Drd1) in mPFC is responsible...

  11. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ...market? In addition to all of the documents you submit, you will need to present a "chalk talk." However...mini-organs or organoids, neither of these can substitute for a liver transplant. My lab will combine bioengineered...

  12. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ...directly accomplish the base edit or to create the substrate for a base excision repair pathway. The team behind...UNG enzyme was modified to recognize T and/or C substrates. The HuidaGene Therapeutics team has deposited...

  13. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...multiple myeloma drug mezigdomide and ubiquitination substrate Ikaros (IKZF1ZF2) (B). Image adapted from Kroupova... Tag catalyze a luminescent reaction with the substrate furimazine. Right: Demonstration of PILATeS. Cell...

  14. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    .../dev team rarely, if ever, sees cover letters submitted for their entry-level jobs and does not require...there were examples of great hires who hadn’t submitted a cover letter; their experience and skills had...

  15. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ... an organism uses only a subset of codons, it only needs to produce a subset of charged tRNAs and therefore...

  16. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...transductions. Cell lines should be routinely subcultured at densities that are low enough to prevent confluency...behavior. Signs of a mycoplasma contamination can be subtle and may include reduced saturation density, decreased...

  18. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...N-terminus, but this hardly reduces its size. The substantial size of GFP may interfere with the activity or...generated several functional heterotrimeric G-alpha subunit fusions that do not tolerate N- and C-terminal ...

  19. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...are identified by PCR. Then, if necessary for subsequent experiments, the floxed neoR gene is removed ..., and are therefore able to infect only a small subset of the cells used in a given experiment. For this...

  20. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ...are already done. Intracellular labeling can be subdivided into two categories, depending on the location...DNA during DNA synthesis and then diluted with subsequent rounds of DNA synthesis (cell divisions).   ...
Showing: 301 - 320 of 578 results