Addgene

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Showing: 301 - 320 of 382 results

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Transferable Skills Guide: Identifying Your Transferable Skills

Type

Blog Post

... gaps in scientists' transferrable skills. In describing the limitations of the study, the authors noted...
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

Type

Blog Post

...quantification (Kerppola, 2006, 2013). These are described in more detail below: Fusion protein construction...
The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

Type

Blog Post

...into two components: 𝜃x(t) and 𝜓z(t). 𝜃x(t) describes the exponential decay process we observed, while...
DNA Purification Without a Kit

Type

Blog Post

...plasmid purification and DNA gel extraction method described in Kim and Morrison is nearly identical to that...
A Guide to Starting Your Own Journal Club

Type

Blog Post

...served. I hope that with some of the information described above you feel empowered to start your own journal...
AAVs CREATed for Gene Delivery to the CNS and PNS

Type

Blog Post

...AAV as a tool for gene expression in mammals. Subscribe to Viral Vector Blog Posts Additional Resources...
CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

Type

Blog Post

...established with Bowtie2 using the parameters first described in Kearns et al.: bowtie2 -f -x HG19_GENOME --...
Plasmids 101: Colony PCR

Type

Blog Post

...expected results for the three primers previously described. When using insert-specific primers (1), positive...
Plasmids 101: Protein tags

Type

Blog Post

...examples. The tables list more common tags than are described in the post, but have been categorized to help...
Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

Type

Blog Post

...biochemical properties. In 1995, Roger Y. Tsien described an S65T point mutation that increased the fluorescence...
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

Type

Blog Post

...for reprogramming purposes. In this post, I’ll describe an episomal vector system that increases reprogramming...
Fluorescent Proteins 101: Aptamer Fluorophores

Type

Blog Post

... as the aptamer is bound to a protein fluor. Transcribing and translating a protein fluorophore also takes...
Plasmids 101: Gateway Cloning

Type

Blog Post

...Gateway technology relies on the two reactions described below: The BP Reaction takes place between the...
Using Video to Share Your Science: We Share Science

Type

Blog Post

...provide more than enough time for researchers to describe their science, and for viewers to decide if reading...
Changing Labor Laws Bring Increased Postdoc Wages

Type

Blog Post

...expected for postdocs in many cases should be as described in the notice of the new NRSA levels and in the...
5 Reasons to Use Reddit for Science Communication

Type

Blog Post

...receive via email. The cool features are too many to describe here, but I have over 2,000 notes which Evernote...
Deciphering the Mysteries of Behavior with Viral Vectors

Type

Blog Post

...that were not (Hare et al., 2019). The research described here is only a small selection of the increasing...
What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

Type

Blog Post

...genotype to phenotype studies New organismal systems described at this meeting were extraordinarily diverse. ...
Overcoming the AAV Size Limitation for CRISPR Delivery

Type

Blog Post

...as potent and specific? The two AAV strategies described above showed successful target modification, indicating...
An Inside Look into Addgene's Dev Team

Type

Blog Post

... on a new feature. We start with a story that describes the feature and what the criteria is for accepting...

Showing: 301 - 320 of 382 results

Results per page:

Transferable Skills Guide: Identifying Your Transferable Skills

Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

DNA Purification Without a Kit

A Guide to Starting Your Own Journal Club

AAVs CREATed for Gene Delivery to the CNS and PNS

CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

Plasmids 101: Colony PCR

Plasmids 101: Protein tags

Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

Fluorescent Proteins 101: Aptamer Fluorophores

Plasmids 101: Gateway Cloning

Using Video to Share Your Science: We Share Science

Changing Labor Laws Bring Increased Postdoc Wages

5 Reasons to Use Reddit for Science Communication

Deciphering the Mysteries of Behavior with Viral Vectors

What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

Overcoming the AAV Size Limitation for CRISPR Delivery

An Inside Look into Addgene's Dev Team

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