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Showing: 301 - 320 of 618 results

  3. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...counter 37 °C, 5% CO 2 incubator with shaking platform set to 120 rpm 37 °C bead bath Vortex Stir bar Magnetic...a 37 °C, 5% CO 2 incubator on a shaking platform set to 120 rpm. Pro-Tip Do not use cells that are over...into a clean microcentrifuge tube. Pro-Tip Cells settle quickly and need to be resuspended before sampling...

  5. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    ...Dronpa dimers interact and lock MEK1 in an “off” setting by blocking the active site. Dronpa also produces...few such methods exist and they only work for a subset of kinases. The Problem:  There is no common method...

  7. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ...gene. In this manner, we have created an initial set of 10 plasmids representing 10 human genes and will...Last updated on Oct 14, 2020 by Seth Kasowitz. This post was contributed by the gene editing team at ...

  10. RNA Extraction Without A Kit

    Type
    Blog Post
    ...extraction. Researchers at the NIH in the mid 1980’s set out to develop a protocol that skipped the ultracentrifugation...

  12. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ... Karmella Haynes at Emory recently contributed a set of plasmids to the repository that will help epigenetic...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ...

  14. Addgene: The First Twenty Years

    Type
    Blog Post
    ...blast from the past: Cofounder and CSO Melina Fan setting up Addgene's very first in-house NGS run.   ...approvals. In 2013 we were thrilled to receive the Massachusetts Nonprofit Network Excellence Award for our electronic...

  19. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    ... a small subset of cells? Other proteins may also be utliized as scaffolds, including the set of red fluorescent...recombination at LoxP sites. Placing a lox-STOP-lox cassette upstream of a gene of interest blocks transcription...absent. When Cre is present, it removes the STOP cassette, activating gene expression. Although Cre isn’...
Showing: 301 - 320 of 618 results