We narrowed to 622 results for: Set;

...). A control RFP expression plasmid.  With this set up, all successfully transfected cells will express...

...applications and targeting scope of ABEs David Liu’s lab set out to evolve ABEs with an increased compatibility..., can only target certain sites, or make only a subset of base substitutions.  The need for speed: base...

... welcome changeover from conventional academic settings, these are an eye-opening experience for cultivating...

...mitochondria, because cPYP binds to BoPD. A similar set of newly identified light- and dark- binder plasmid...Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids...genes-of-interest (GOI) into two independent expression cassettes on a single plasmid (Grefen and Blatt, 2012). ...

...population (all barcodes can be amplified using the same sets of forward and reverse primers). The abundance of...

...for resource allocation. Those will allow you to set the amount of transcriptional and translational activity...Christopher Voigt explains it, his lab at the Massachusetts Institute of Technology has been “working on...

...ready for restriction digestion. Digest Your DNA Set up restriction digests for your PCR product and recipient...recipient plasmid to insert. It is also important to set up negative controls in parallel. For instance, a...

...current position at Fred Hutch because her skill set wasn’t matching up with the local job postings. “...

...tracked and quantitatively analyzed by sequencing its set of barcodes. CombiGEM is highly flexible and can ...

...blast from the past: Cofounder and CSO Melina Fan setting up Addgene's very first in-house NGS run.   ...approvals. In 2013 we were thrilled to receive the Massachusetts Nonprofit Network Excellence Award for our electronic...

...your blot with multiple antibodies. You’ll want to set up your blot with a polyvinylidene difluoride (PVDF...

...Dronpa dimers interact and lock MEK1 in an “off” setting by blocking the active site. Dronpa also produces...few such methods exist and they only work for a subset of kinases. The Problem:  There is no common method...

... et al, 2008) occasionally and certainly after a set-up has been changed (for instance after replacing...

...gene. In this manner, we have created an initial set of 10 plasmids representing 10 human genes and will...Last updated on Oct 14, 2020 by Seth Kasowitz. This post was contributed by the gene editing team at ...

...coinfect with multiple viruses to express genes or gene sets over 4.7 kb. Co-infection rates for AAVs are generally...

...activation  and base editing, whereas a different set of mutations (the “V3.1” version) works best for ...

...solution can easily be implemented in an academic lab setting, where student and postdoc turnover happens regularly...

...when thinking about crafting your own poster. What sets a scientific poster apart? First, a good poster ...

...connections This is a site for meeting people. Use the settings to let people see who you are when you look at...

... Karmella Haynes at Emory recently contributed a set of plasmids to the repository that will help epigenetic...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ...