...coinfect with multiple viruses to express genes or gene sets over 4.7 kb. Co-infection rates for AAVs are generally...

... range of DNA edits.   Cas3 – The shredder What sets Cas3 apart from Cas9 and almost all other Cas enzymes...

...blast from the past: Cofounder and CSO Melina Fan setting up Addgene's very first in-house NGS run.   ...approvals. In 2013 we were thrilled to receive the Massachusetts Nonprofit Network Excellence Award for our electronic...

...your blot with multiple antibodies. You’ll want to set up your blot with a polyvinylidene difluoride (PVDF...

... et al, 2008) occasionally and certainly after a set-up has been changed (for instance after replacing...

...gene. In this manner, we have created an initial set of 10 plasmids representing 10 human genes and will...Last updated on Oct 14, 2020 by Seth Kasowitz. This post was contributed by the gene editing team at ...

...current position at Fred Hutch because her skill set wasn’t matching up with the local job postings. “...

...activation  and base editing, whereas a different set of mutations (the “V3.1” version) works best for ...

... Karmella Haynes at Emory recently contributed a set of plasmids to the repository that will help epigenetic...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ...

...its surroundings and being shaped by them. Another set of highly influential microenvironment cells are ...

...deposit to Addgene. We are also lauded by another set of stakeholders - the tech transfer community. In...

...“We hope to use CRISPR libraries from Addgene to set up assays to see what molecules or proteins are involved...

...extraction. Researchers at the NIH in the mid 1980’s set out to develop a protocol that skipped the ultracentrifugation...

...solution can easily be implemented in an academic lab setting, where student and postdoc turnover happens regularly...

...when thinking about crafting your own poster. What sets a scientific poster apart? First, a good poster ...

...connections This is a site for meeting people. Use the settings to let people see who you are when you look at...

...Dronpa dimers interact and lock MEK1 in an “off” setting by blocking the active site. Dronpa also produces...few such methods exist and they only work for a subset of kinases. The Problem:  There is no common method...

...tracked and quantitatively analyzed by sequencing its set of barcodes. CombiGEM is highly flexible and can ...

... a small subset of cells? Other proteins may also be utliized as scaffolds, including the set of red fluorescent...recombination at LoxP sites. Placing a lox-STOP-lox cassette upstream of a gene of interest blocks transcription...absent. When Cre is present, it removes the STOP cassette, activating gene expression. Although Cre isn’...

...for this transition. Second, a coach will help you set goals and then hold you accountable for carrying ...