We narrowed to 731 results for: Sis

...TnpB (named ISYmu1) to the popular plant model Arabidopsis (Weiss et al., 2025). By co-delivering TRV1 with...editor for transgene-free germline editing in Arabidopsis. Nature Plants, 11(5), 967–976. https://doi.org...Figure 3: Using LARRYv2 libraries for state-fate analysis in ex vivo HSC expansion cultures. A) Experimental...

...the flanks of each ligation product, which is the basis for the repeatability of the method. Note that the... 4. Josse J, Kaiser AD, Kornberg A. Enzymatic synthesis of deoxyribonucleic acid. VIII. Frequencies of...Swartz MN, Trautner TA, Kornberg A. Enzymatic synthesis of deoxyribonucleic acid. XI. Further studies ...

...adapted for a wide variety of applications - as mutagenesis tools for genetic studies (e.g. using mariner...Insertion of Transposable Elements by Guide RNA-Assisted Targeting) by the Sternberg group, and CAST (CRISPR-associated...SE, Sternberg SH, Fernández IS (2020) Structural basis of DNA targeting by a transposon-encoded CRISPR–...

...BiFC in your cellular system. BiFC competition analysis can be used in lieu of negative controls (Kerppola...Bimolecular Fluorescence Complementation (BiFC) Analysis of Protein Interactions in Live Cells. Cold Spring...Kim Y, Jung JP, Pack C-G, Huh W-K (2018) Global analysis of protein homomerization in Saccharomyces cerevisiae...

...full-spectrum analysis, rather than just the absorbances at 230, 260, and 280 nm. Full-spectrum analysis allows... Tris-buffered solution tends to produce more consistent readings — just be sure it is compatible with...

...to 1:32. Each individual step of the series is consistent at 1:2. When preparing a dilution series, use...non-linear and you should use a different method of analysis. Different fields and assays have varying criteria...run.  When setting up your dilution series, use consistent dilution factors, change pipette tips regularly...

...the detection of rare sequences and single cell analysis. It is also being used in the detection of microbes... al. "Detection of circulating Mycobacterium tuberculosis-specific DNA by droplet digital PCR for vaccine...vaccine evaluation in challenged monkeys and TB diagnosis." Emerging microbes & infections 7.1 (2018): 1...

...non-dividing cells. The first lentiviral vector system consisted of three plasmids: the packaging, envelope, and...other reporters, and they showed that expression persisted for at least thirty days. Although adeno-associated...integrate into the genome, they could promote oncogenesis by altering local gene expression. Self-inactivating...

...minigenome, the viral polymerase initiates RNA synthesis at the 3’ end of the minigenome to produce the...These products can be detected by Northern blot analysis. In addition, the polymerase will use the minigenome...mRNA can be detected directly by Northern blot analysis or RT-PCR. Uses of the minigenome system Minigenome...

...tracrRNA, crRNA guides are only ~42 nt long. Direct synthesis of these crRNAs should be significantly cheaper...rates, determined using sophisticated genome-wide analysis. At many of its computationally predicted off-...at Addgene. 3. Kim, Daesik, et al. Genome-wide analysis reveals specificities of Cpf1 endonucleases in...

...immunoprecipitation (locus-specific ChIP). These methods consist of insertional chromatin immunoprecipitation (iChIP...on development of technologies for biochemical analysis of genome functions such as transcription and ...technologies. The locus-specific ChIP technologies consisting of iChIP and enChIP are recent examples. We also...

...Properties of FLP Recombinase Evolved by Cycling Mutagenesis.” Nature biotechnology 16(7): 657–62. PubMed ...Deleter Mouse in C57Bl/6 Based on FLPo Recombinase.” Genesis 48(8): 512–20. PubMed PMID: 20506501. 6. Logie,...Synapses Are Repeatedly Assembled and Removed at Persistent Sites In Vivo.” Neuron 89(4): 756–69. PubMed ...

...proteins within the bacterial genome. From this analysis, they identified 53 potential candidate genes ...within Cas13a are essential for RNA cleavage, consistent with known roles for HEPN domains in other proteins...located at the 3’ end of the spacer sequence and consists of a single A, U, or C base pair. In bacteria,...

...CRISPR corner New from the viral service Faster catalysis after yeast-based protein evolution By Joanne ...evolution platform to generate TEV protease variants. Catalysis by TEV protease allows a transcription factor ...CRISPR/Cas Toolkit for plants includes 95 plasmids consisting of CRISPR/Cas nucleases, base editors, gRNA backbones...

...singletons (reads that only appear once) in our analysis. Many pipelines toss these singletons out, and...stranger - Finding mentors for the meeting Just like analysis pipelines for DNA that group similar sequences...variability was organic, as some people need less assistance than others, but we did have some participants...

...the accompanying sequencing data collection and analysis eventually outpaced our software, even with continuous...singular purpose (ex. adapter trimming, quality score analysis, k-mer counting, or sequence assembly), but the...would handle plasmid sequences on a 96-well plate basis, but for rapid testing and refinements, we specified...

...concentration, ionic strength and pH, affect binding the lysis buffer may need to be adjusted depending on the ...include protease and phosphatase inhibitors in the lysis buffer, keep samples on ice, and perform the IP ...For mass spectrometry, elute in a urea-containing lysis buffer. Alternatively, the protein can also be eluted...

...pTargetF system Our original pCas/pTargetF system consists of one plasmid that expresses the S. pyogenes ...engineering case study of N-acetylglucosamine biosynthesis, we constructed a strain library with 1-11 copies... modified pCas/pTargetF system for CRISPR-Cas9-assisted genome editing in Escherichia coli. Acta Biochimica...

...important models for solutions to the coral reef crisis. I like how they are using protocols.io to create...disparate environments so can be used study the genetic basis of evolution in immunology, neurobiology, and more...lab has the capability) with receptor mediated endocytosis. They used this to edited those brown marmorated...

...They first created a defective chloramphenicol resistance gene (CamR) by introducing a point mutation (... adenine base editor would restore antibiotic resistance. To find such a protein, they created a mutant...any restriction enzyme (RE) recognition site, RE analysis of PCR product could be used to detect success...