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We narrowed to 731 results for: Sis;

Showing: 301 - 320 of 731 results

  1. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...gene networks for the detection of antibiotic resistance and strain-specific diagnostics for the Ebola...Pardee and Alexander Green! Keith Pardee is an Assistant professor in the Leslie Dan Faculty of Pharmacy...the environment.     Alexander A. Green is an Assistant professor at the Biodesign Center for Molecular...

  2. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    ...silencing of self-genes. Dr. Mello presented a hypothesis that these pathways are involved in RNA based...address the growing clinical problem of antibiotic resistant bacteria. See the picture to the left for his ... vectors are engineered to replace the drug resistantance genes in the bacteria thus making the bacteria...

  3. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    ...living cells. Essentially this reporter construct consists of one substrate (the histone-derived peptide)... of these sites was controlled, the reporter consistently showed significantly different FRET levels between...complex or multifactorial diseases, enabling faster diagnosis and treatment and improving patients lives. References...

  4. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...one.   SMASh Small molecule-assisted shutoff (SMASh) functions on the basis of both a degron and a viral...Given its popularity and widespread usage, a proteolysis-targeting chimera (PROTAC) was developed for ...

  5. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...knowledge of protein structure and saturation mutagenesis. mK-GO matures from green to red, and the FT ...absorption spectra). Subach et al’s directed mutagenesis also produced the three separate timers shown...Age-dependent preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed...

  6. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...able to study many cellular processes, including mitosis, mitochondrial fission/fusion, nuclear import, ...several groups have designed modular toolkits to assist with tagging genomic loci in other model systems...including mammalian cells. CRISPaint (CRISPR-assisted insertion tagging), developed by the Hornung Lab...

  7. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...refers to the second step of this process: the synthesis of proteins from mRNA and the addition of post-translational...Overall the general strategy for protein expression consists of transfecting cells with your DNA template of...the infected host cell is eventually lysed. Cell lysis halts protein production, but there are non-lytic...

  8. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    ...-transformed cells yielded rates of targeted mutagenesis similar to those achieved by isogenic AdVs encoding...Elsevier, pp 39–67 Ding Q, Strong A, Patel KM, Ng S-L, Gosis BS, Regan SN, Cowan CA, Rader DJ, Musunuru K (2014...CRISPR/Cas9 nuclease complexes induces targeted mutagenesis in a diverse array of human cells. Sci Rep 4:...

  9. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...results you need.  Whichever method you choose, resist the temptation to over interpret your results: .... PMID: 15833050. Ma L, Chung WK. Quantitative analysis of copy number variants based on real-time LightCycler...amplification of Plasmodium falciparum multidrug resistance-1 gene (pfmdr1). J Parasitol. 2011 Oct;97(5):...

  10. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...cloning rings to isolate colonies of antibiotic-resistant cells; during the time required to grease, place... below) is simple: After obtaining antibiotic-resistant colonies, use a Sharpie to circle 12-48 individual...specific wells from the 48-well plate for further analysis. To assess CRISPR/Cas9 knockout efficacy, we have...

  11. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ...to 1:32. Each individual step of the series is consistent at 1:2. When preparing a dilution series, use...non-linear and you should use a different method of analysis. Different fields and assays have varying criteria...run.  When setting up your dilution series, use consistent dilution factors, change pipette tips regularly...

  12. MXS Chaining

    Type
    Blog Post
    ...the flanks of each ligation product, which is the basis for the repeatability of the method. Note that the... 4. Josse J, Kaiser AD, Kornberg A. Enzymatic synthesis of deoxyribonucleic acid. VIII. Frequencies of...Swartz MN, Trautner TA, Kornberg A. Enzymatic synthesis of deoxyribonucleic acid. XI. Further studies ...

  13. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...the detection of rare sequences and single cell analysis. It is also being used in the detection of microbes... al. "Detection of circulating Mycobacterium tuberculosis-specific DNA by droplet digital PCR for vaccine...vaccine evaluation in challenged monkeys and TB diagnosis." Emerging microbes & infections 7.1 (2018): 1...

  14. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    ...adapted for a wide variety of applications - as mutagenesis tools for genetic studies (e.g. using mariner...Insertion of Transposable Elements by Guide RNA-Assisted Targeting) by the Sternberg group, and CAST (CRISPR-associated...SE, Sternberg SH, Fernández IS (2020) Structural basis of DNA targeting by a transposon-encoded CRISPR–...

  16. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...non-dividing cells. The first lentiviral vector system consisted of three plasmids: the packaging, envelope, and...other reporters, and they showed that expression persisted for at least thirty days. Although adeno-associated...integrate into the genome, they could promote oncogenesis by altering local gene expression. Self-inactivating...

  17. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type
    Blog Post
    ...minigenome, the viral polymerase initiates RNA synthesis at the 3’ end of the minigenome to produce the...These products can be detected by Northern blot analysis. In addition, the polymerase will use the minigenome...mRNA can be detected directly by Northern blot analysis or RT-PCR. Uses of the minigenome system Minigenome...

  18. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ...tracrRNA, crRNA guides are only ~42 nt long. Direct synthesis of these crRNAs should be significantly cheaper...rates, determined using sophisticated genome-wide analysis. At many of its computationally predicted off-...at Addgene. 3. Kim, Daesik, et al. Genome-wide analysis reveals specificities of Cpf1 endonucleases in...

  19. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    ...immunoprecipitation (locus-specific ChIP). These methods consist of insertional chromatin immunoprecipitation (iChIP...on development of technologies for biochemical analysis of genome functions such as transcription and ...technologies. The locus-specific ChIP technologies consisting of iChIP and enChIP are recent examples. We also...

  20. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...that a plasmid can transfer to, replicate in, and persist in. Some plasmids have a narrow host range, while...host range recombinant vectors Replicons, which consist of a plasmid’s ori and all of its control elements...scientists can incorporate interchangeable antibiotic resistance cassettes (Prior et al. 2010), inducible promoters...
Showing: 301 - 320 of 731 results