Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 738 results for: Sis;

Showing: 301 - 320 of 738 results

  2. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    ...graduate students who work as teaching or research assistants to join unions. The topic of unionization continues...of Howell Communications Philip Brenner, PhD - Assistant Professor of Sociology. University of Massachusetts...PhD - Biotechnology Communications Specialist at AsisChem Inc. and Social Media Content Specialist at Thermo...

  3. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...gene networks for the detection of antibiotic resistance and strain-specific diagnostics for the Ebola...Pardee and Alexander Green! Keith Pardee is an Assistant professor in the Leslie Dan Faculty of Pharmacy...the environment.     Alexander A. Green is an Assistant professor at the Biodesign Center for Molecular...

  4. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    ...silencing of self-genes. Dr. Mello presented a hypothesis that these pathways are involved in RNA based...address the growing clinical problem of antibiotic resistant bacteria. See the picture to the left for his ... vectors are engineered to replace the drug resistantance genes in the bacteria thus making the bacteria...

  5. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    ...living cells. Essentially this reporter construct consists of one substrate (the histone-derived peptide)... of these sites was controlled, the reporter consistently showed significantly different FRET levels between...complex or multifactorial diseases, enabling faster diagnosis and treatment and improving patients lives. References...

  6. Hot Plasmids: Summer 2025

    Type
    Blog Post
    ...TnpB (named ISYmu1) to the popular plant model Arabidopsis (Weiss et al., 2025). By co-delivering TRV1 with...editor for transgene-free germline editing in Arabidopsis. Nature Plants, 11(5), 967–976. https://doi.org...Figure 3: Using LARRYv2 libraries for state-fate analysis in ex vivo HSC expansion cultures. A) Experimental...

  7. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...one.   SMASh Small molecule-assisted shutoff (SMASh) functions on the basis of both a degron and a viral...Given its popularity and widespread usage, a proteolysis-targeting chimera (PROTAC) was developed for ...

  8. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...able to study many cellular processes, including mitosis, mitochondrial fission/fusion, nuclear import, ...several groups have designed modular toolkits to assist with tagging genomic loci in other model systems...including mammalian cells. CRISPaint (CRISPR-assisted insertion tagging), developed by the Hornung Lab...

  9. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...cloning rings to isolate colonies of antibiotic-resistant cells; during the time required to grease, place... below) is simple: After obtaining antibiotic-resistant colonies, use a Sharpie to circle 12-48 individual...specific wells from the 48-well plate for further analysis. To assess CRISPR/Cas9 knockout efficacy, we have...

  10. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...knowledge of protein structure and saturation mutagenesis. mK-GO matures from green to red, and the FT ...absorption spectra). Subach et al’s directed mutagenesis also produced the three separate timers shown...Age-dependent preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed...

  11. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...refers to the second step of this process: the synthesis of proteins from mRNA and the addition of post-translational...Overall the general strategy for protein expression consists of transfecting cells with your DNA template of...the infected host cell is eventually lysed. Cell lysis halts protein production, but there are non-lytic...

  12. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    ...-transformed cells yielded rates of targeted mutagenesis similar to those achieved by isogenic AdVs encoding...Elsevier, pp 39–67 Ding Q, Strong A, Patel KM, Ng S-L, Gosis BS, Regan SN, Cowan CA, Rader DJ, Musunuru K (2014...CRISPR/Cas9 nuclease complexes induces targeted mutagenesis in a diverse array of human cells. Sci Rep 4:...

  13. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...results you need.  Whichever method you choose, resist the temptation to over interpret your results: .... PMID: 15833050. Ma L, Chung WK. Quantitative analysis of copy number variants based on real-time LightCycler...amplification of Plasmodium falciparum multidrug resistance-1 gene (pfmdr1). J Parasitol. 2011 Oct;97(5):...

  15. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ...to 1:32. Each individual step of the series is consistent at 1:2. When preparing a dilution series, use...non-linear and you should use a different method of analysis. Different fields and assays have varying criteria...run.  When setting up your dilution series, use consistent dilution factors, change pipette tips regularly...

  16. MXS Chaining

    Type
    Blog Post
    ...the flanks of each ligation product, which is the basis for the repeatability of the method. Note that the... 4. Josse J, Kaiser AD, Kornberg A. Enzymatic synthesis of deoxyribonucleic acid. VIII. Frequencies of...Swartz MN, Trautner TA, Kornberg A. Enzymatic synthesis of deoxyribonucleic acid. XI. Further studies ...

  17. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...the detection of rare sequences and single cell analysis. It is also being used in the detection of microbes... al. "Detection of circulating Mycobacterium tuberculosis-specific DNA by droplet digital PCR for vaccine...vaccine evaluation in challenged monkeys and TB diagnosis." Emerging microbes & infections 7.1 (2018): 1...

  18. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    ...adapted for a wide variety of applications - as mutagenesis tools for genetic studies (e.g. using mariner...Insertion of Transposable Elements by Guide RNA-Assisted Targeting) by the Sternberg group, and CAST (CRISPR-associated...SE, Sternberg SH, Fernández IS (2020) Structural basis of DNA targeting by a transposon-encoded CRISPR–...

  20. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...non-dividing cells. The first lentiviral vector system consisted of three plasmids: the packaging, envelope, and...other reporters, and they showed that expression persisted for at least thirty days. Although adeno-associated...integrate into the genome, they could promote oncogenesis by altering local gene expression. Self-inactivating...
Showing: 301 - 320 of 738 results