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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 616 results for: TIL

Showing: 301 - 320 of 616 results

  4. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...engineering world by storm owing to its ease of use and utility in a wide variety of organisms.  While much of ...X (2019) Rescue of a plant cytorhabdovirus as versatile expression platforms for planthopper and cereal...

  5. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ...exist for this, the most common of which is the utilization of phosphomimetic residues. The phosphorylated...tRNA that recognizes a specific ‘open’ codon and utilizes this triplet to incorporate phosphoserine A Sep-tRNA...

  8. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...cells. Flexible tagging: Choose untagged or add a versatile N- or C-terminal HA tag. Selectable and stable... embryonic development and cancer. But there is still much to learn about their myriad functions and therapeutic...

  9. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...ve reduced the chance of off-target effects, you still need to do your due diligence to ensure they are...here. For some applications, this is ok, but it still must be controlled for and acknowledged as a potential...

  10. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...with a light-pulse at one wavelength and stay on until a second pulse of light, with a different wavelength... number of strategies have been developed for utilizing light to control GPCR activation and downstream...

  11. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...how a virus enters the cell. Different serotypes utilize a number of different receptors, co-receptors, ...cells express your gene. Off-target expression can still occur (Fig. 3) - for example, some neurons can transiently...

  12. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...diverse biological entities on our planet, but we still know collectively little about how they infect and...be understood. Therefore, in addition to their utility in the health sector, phages represent a reservoir...

  13. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    ...and therefore it is important that the digest go until completion. The time required for complete digestion...colonies are a result of uncut empty plasmid, you will still have colonies when you do not add ligase. If the...

  14. Finding Your Perfect Job After University

    Type
    Blog Post
    ...research in a lab in California. I felt then, and still feel now, that it is important to take advantage...often don’t tell you what company a job is with until just before the interview). This wasn’t exactly ...

  15. Centrifugation

    Type
    Protocol
    ...been placed in the centrifuge before you and may still be on the equipment. Before using the centrifuge...

  16. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ... directly injecting DNA into the pronuclei of fertilized eggs, which leads to random incorporation of ...genome after injection into the cytoplasm of a fertilized egg, a process which is inefficient when DNA ...

  17. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...’ve found that the advice in these resources is still valuable for anyone in the lab though, including...but didn’t realize I was interested in microbes until the end of my second year! Conclusion and other...

  19. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ...cells in the population, so some of the cells will still appear green despite having active Cas9 (see tip...site in the middle of the PCR product, you would still be able to identify the cleavage product. 3. The...
Showing: 301 - 320 of 616 results