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  1. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ...exist for this, the most common of which is the utilization of phosphomimetic residues. The phosphorylated...tRNA that recognizes a specific ‘open’ codon and utilizes this triplet to incorporate phosphoserine A Sep-tRNA...

  4. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...ve reduced the chance of off-target effects, you still need to do your due diligence to ensure they are...here. For some applications, this is ok, but it still must be controlled for and acknowledged as a potential...

  5. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...with a light-pulse at one wavelength and stay on until a second pulse of light, with a different wavelength... number of strategies have been developed for utilizing light to control GPCR activation and downstream...

  6. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...how a virus enters the cell. Different serotypes utilize a number of different receptors, co-receptors, ...cells express your gene. Off-target expression can still occur (Fig. 3) - for example, some neurons can transiently...

  7. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...diverse biological entities on our planet, but we still know collectively little about how they infect and...be understood. Therefore, in addition to their utility in the health sector, phages represent a reservoir...

  8. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    ...and therefore it is important that the digest go until completion. The time required for complete digestion...colonies are a result of uncut empty plasmid, you will still have colonies when you do not add ligase. If the...

  9. Finding Your Perfect Job After University

    Type
    Blog Post
    ...research in a lab in California. I felt then, and still feel now, that it is important to take advantage...often don’t tell you what company a job is with until just before the interview). This wasn’t exactly ...

  10. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ...cells in the population, so some of the cells will still appear green despite having active Cas9 (see tip...site in the middle of the PCR product, you would still be able to identify the cleavage product. 3. The...

  13. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ... directly injecting DNA into the pronuclei of fertilized eggs, which leads to random incorporation of ...genome after injection into the cytoplasm of a fertilized egg, a process which is inefficient when DNA ...

  14. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...’ve found that the advice in these resources is still valuable for anyone in the lab though, including...but didn’t realize I was interested in microbes until the end of my second year! Conclusion and other...

  17. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ... cancer, multiple immunotherapy targets can be utilized at the same time. A combination of tolerance marker...the development of immunotherapeutics. There are still many important questions in this exciting field ...

  18. Typing CRISPR Systems

    Type
    Blog Post
    ...we call CRISPR, by any other name would…probably still edit genomes. Understanding the different types... in archaea, though Class 1 remains largely underutilized compared to Class 2. Class 1 Despite their incredible...

  19. Which Fluorescence Microscopy Techniques is Best for Me?

    Type
    Blog Post
    ...Sheet Microscopy Light Sheet Microscopy typically utilizes a configuration of two or more objectives to create...monolayers of live cells with fluorescently labelled, motile structures These samples are thin enough to sit...

  20. Centrifugation

    Type
    Protocol
    ...been placed in the centrifuge before you and may still be on the equipment. Before using the centrifuge...
Showing: 301 - 320 of 607 results