We narrowed to 614 results for: TIL;

... in the middle of your insert. Gateway cloning utilizes λ integrase to catalyze directional cloning of... Brook Pyhtila...

... models, and new Cre-lox based technologies are still being developed today. In this post, I will highlight...labeling system - a perfect example of the continued utility of Cre-lox. Check out our previous blog post, Plasmids...

...been placed in the centrifuge before you and may still be on the equipment. Before using the centrifuge...

...ve reduced the chance of off-target effects, you still need to do your due diligence to ensure they are...here. For some applications, this is ok, but it still must be controlled for and acknowledged as a potential...

...with a light-pulse at one wavelength and stay on until a second pulse of light, with a different wavelength... number of strategies have been developed for utilizing light to control GPCR activation and downstream...

...how a virus enters the cell. Different serotypes utilize a number of different receptors, co-receptors, ...cells express your gene. Off-target expression can still occur (Fig. 3) - for example, some neurons can transiently...

...cells. Flexible tagging: Choose untagged or add a versatile N- or C-terminal HA tag. Selectable and stable... embryonic development and cancer. But there is still much to learn about their myriad functions and therapeutic...

...diverse biological entities on our planet, but we still know collectively little about how they infect and...be understood. Therefore, in addition to their utility in the health sector, phages represent a reservoir...

...and therefore it is important that the digest go until completion. The time required for complete digestion...colonies are a result of uncut empty plasmid, you will still have colonies when you do not add ligase. If the...

...research in a lab in California. I felt then, and still feel now, that it is important to take advantage...often don’t tell you what company a job is with until just before the interview). This wasn’t exactly ...

...percentage of fluorescent cells, these cells can still be used in downstream applications. Safety Warnings...

...and circularization of dsDNA (6). Less commonly utilized in vitro, Taq DNA ligase will ligate only nicks...Despite this higher fidelity, Taq DNA Ligase can still detectably ligate many T:G, T:T, and A:C mismatches...

...cells in the population, so some of the cells will still appear green despite having active Cas9 (see tip...site in the middle of the PCR product, you would still be able to identify the cleavage product. 3. The...

...Twitter.   References Arribere JA, Bell RT, Fu BXH, Artiles KL, Hartman PS, Fire AZ (2014) Efficient Marker-Free...Schmidt H, Krause MW, Seydoux G (2014) Scalable and Versatile Genome Editing Using Linear DNAs with Microhomology...

...invisible to naked eyes and hence cannot be detected until it leads to contamination. Another source of the...regularly and dampness of any kind should be avoided. Distilled water provided to maintain the humidity should...

... directly injecting DNA into the pronuclei of fertilized eggs, which leads to random incorporation of ...genome after injection into the cytoplasm of a fertilized egg, a process which is inefficient when DNA ...

...’ve found that the advice in these resources is still valuable for anyone in the lab though, including...but didn’t realize I was interested in microbes until the end of my second year! Conclusion and other...

...time by shortening or skipping many steps and will still get enough colonies for your next step. Remember...

...Sheet Microscopy Light Sheet Microscopy typically utilizes a configuration of two or more objectives to create...monolayers of live cells with fluorescently labelled, motile structures These samples are thin enough to sit...

...immune response, but the study of these dynamics was still laborious as epifluorescence microscopy wasn’t able... provide quantitative measurements of cellular motility, interactions, and response dynamics. In the past...