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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 772 results for: Vars;

Showing: 301 - 320 of 772 results

  1. New Viral Vectors - Summer 2024

    Type
    Blog Post
    ...also added AAV1 and AAV5 preparations of several variants of a new optogenetic tool called PdCO, deposited...offering AAV1 and AAV5 preparations of several variants of a new optogenetic tool called PdCO.  This is...Hub What is a Virus (and a Viral Vector)?  AAV Variables that Matter...

  2. Editor's Choice, October 2016

    Type
    Blog Post
    ...have a variety of downstream effects including possibly decreasing the number of postdocs various academic...Program in Biological and Biomedical Sciences at Harvard University. Ben and Niroshi have tons of great ...

  3. Grad School Advice Part 1: Picking a Lab and a Project

    Type
    Blog Post
    ... Biological and Biomedical Sciences program at Harvard University to get the scoop on the ups and down...candidate in the Program in Genetics and Genomics at Harvard Medical School. He studies developmental gene regulation...of the PhD program in Genetics and Genomics at Harvard Medical School, and is currently a fellow in clinical...

  4. Editor's Choice, July 2016

    Type
    Blog Post
    ...editing when compared to the most commonly used Cas9 variant, SpCas9. These properties, combined with other ...in the post, may make Cpf1 the preferred CRISPR variant going forward.   Special Delivery Fluorophore Targeting...describes how to target and use FRET flurophores for a variety of applications. While editing this post, I learned...

  5. Grad School Advice Part 2: Building Community

    Type
    Blog Post
    ... Biological and Biomedical Sciences program at Harvard University and pick their brains on how they've...candidate in the Program in Genetics and Genomics at Harvard Medical School. He studies developmental gene regulation...of the PhD program in Genetics and Genomics at Harvard Medical School, and is currently a fellow in clinical...

  7. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    ...backbones containing GFP variants used by Sattarzadeh et al., as well as a wide variety of other fluorescent...imaging process worked well with the common GFP variants EGFP, mGFP4-T, and S65T GFP, and no red color ...

  8. IDP and your PI: A Roadmap for Career Planning and Personal Development

    Type
    Blog Post
    ...opportunities or to find oneself unsure of the next steps. Harvard PI Angela DePace has put personal development at...conversation. As the conversation proceeds through the various sections, the trainee speaks first for each section...assessment or critique. When trainees ask for help with various issues, the PI is responsible for determining meaningful...

  9. Celebrating Accomplishments in the Lab

    Type
    Blog Post
    ...London), Dr. Connie Cepko (Harvard Medical School), and Dr. George Church (Harvard Medical School) for taking...Church and his family spend family reunions in various wildernesses and enjoys “three generations walk...

  10. Supporting Rare Disease Research with BeHeard 2017

    Type
    Blog Post
    ... these various model systems should allow Nabavi and coworkers to test inhibitors of a variety of signaling...the lungs is relatively well known, there are a variety of lesser known mesothelioma subsets. Peritoneal...

  12. Build Your CRISPR Vocabulary

    Type
    Blog Post
    ... is composed of identical repeat sequences and variable spacer sequences. The array is transcribed and...in the non-target strand of DNA to be cut, which varies between CRISPR systems. Cas proteins won’t cut ... CRISPR by mutating these sequences. Pro tip! Various labs have created Cas9 enzymes with looser PAM ...

  13. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

    Type
    Blog Post
    ...efficiency in PAM-in and PAM-out configurations with varied nick distances (40 - 130 nt). From these results...H840A are potent editors, but their indel profiles vary. In the PAM-out configuration, D10A tends to generate...Using a D10A nickase to insert an EcoRI site in various spots between the nick sites, they observed >20%...

  14. Year of the Camelid: Antibody Style

    Type
    Blog Post
    ...developing transgenic mice that express camelid variable domains (aka nanomice), which gives researchers...modifications made to generate the nanomouse. (VHH = variable domains of heavy chain only antibodies) (B) Nanomice... camelid mice and llamas neutralize SARS-CoV-2 variants. Nature 595:278–282. https://doi.org/10.1038/s41586...

  15. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ...Streptomyces, a prokaryotic genus capable of producing a variety of natural products. Recently, this group optimized...base-editing efficiency using XMAS-TREE-based methods at various genomic loci in four different categories. ...  Various Addgenies...

  17. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    ... confirm expression in target cells. While the various AAV serotypes can be used to achieve different ... plasmids now packaged in AAVrg. jGCaMP7s, the variant with the most sensitive response to action potentials...to measure glutamate levels. iGluSnFR.A184S, a variant with higher affinity to glutamate, has been packaged...

  18. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    ...pH-insensitive RFP variant, DsRed.T3, that’s connected to a pH-sensitive GFP variant, super ecliptic pHluorin...fixed cells. Find the mitochondria-targeted Keima variant at Addgene! mRFP-GFP-LC3 tandem fluorescent protein...

  19. Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation

    Type
    Blog Post
    ...Professor at the Lab for Innovation Science at Harvard, wanted to see how new tools drive innovation in... their gene sequence may have slight or large variations depending on how closely related these species...percentage were not good predictors. There is not much variation in GC content between many organisms and DNA synthesis...

  20. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    ...to the beat of their own drum. They have a wide variety of possible genome types that can be either double...positive sense ssRNA, or negative sense ssRNA) and varying methods of replication. In fact, an entire classification...proteins. RNA viruses replicate their genomes using a variety of RNA or DNA intermediaries, depending on the ...
Showing: 301 - 320 of 772 results