-
Pooled Library#208054PurposepGL0_151 [RS421] is a library of broad host range prokaryotic promoters-RBS regulatory sequences formatted as a golden gate assembly Level 0 part.DepositorUseSynthetic BiologyAvailable sinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only
-
APA Variant and Reference Libraries
Pooled Library#1000000214DepositorAvailable sinceMarch 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_hCAR.Variant3
Plasmid#209133PurposeThe ORF of human constitutive androstane receptor (CAR), variant 3 cDNA amplified from the RT human liver RNA was cloned at the NheI/HindIII sites of the pcDNA3.1(+), the mammalian expression vector.DepositorInsertconstitutive androstane receptor (CAR), Variant 3 (NR1I3 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2_IRES2_EGFP_KCNQ2 var4
Plasmid#173154PurposeFor mammalian expression of KCNQ2 and creating variantsDepositorInsertKCNQ2 (KCNQ2 Human)
UseTagsIRES2-EGFPExpressionMammalianMutationPromoterSimian CMV IE94Available sinceSept. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
VRQR variant
Plasmid#138562PurposeExpresses human codon-optimized SpCas9-VRQR and blasticidin resistance: EFS promoter-VRQR-NLS-FLAG-P2A-BSDDepositorInsertVRQR
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationD1135V, G1218R, R1335Q, T1337RPromoterEFSAvailable sinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP VARP
Plasmid#42312DepositorInsertVARP (ANKRD27 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCS2_IRES2_CyOFP1_KCNQ2 var4
Plasmid#173155PurposeFor mammalian expression of KCNQ2 and creating variantsDepositorInsertKCNQ2 (KCNQ2 Human)
UseTagsIRES2-CyOFP1ExpressionMammalianMutationPromoterSimian CMV IE94Available sinceSept. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_SNIPR_CD8alpha variant 2
Plasmid#188362PurposeLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factorDepositorInsertPGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMV-VARNAM
Plasmid#115552PurposeRed fluorescent voltage indicator for mammalian expressionDepositorInsertVARNAM
UseTagsGolgi and ER export signalExpressionMammalianMutationPromoterCMV IE94Available sinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-EnvARGCD
Plasmid#100800Purposeexpress fusion protein of EnvA (ASLV-A envelope protein) extracellular and transmembrane domains with rabies virus SAD B19 strain glycoprotein intracellular domainDepositorInsertfusion protein of EnvA extracellular and transmembrane domain with rabies virus glycoprotein intracellular domain
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceDec. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_SNIPR_CD8alpha variant 4
Plasmid#188364PurposeLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant4-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factorDepositorInsertPGK_antiCD19_CD8alpha-variant4-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
VQR variant
Plasmid#138560PurposeExpresses human codon-optimized SpCas9-VQR and blasticidin resistance: EFS promoter-VQR-NLS-FLAG-P2A-BSDDepositorInsertVQR
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationD1135V, R1335Q, T1337RPromoterEFSAvailable sinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
QQR1 variant
Plasmid#138564PurposeExpresses human codon-optimized SpCas9-QQR1 and blasticidin resistance: EFS promoter-QQR1-NLS-FLAG-P2A-BSDDepositorInsertQQR1
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationG1218R, N1286Q, I1331F, D1332K, R1333Q, R1335Q, T…PromoterEFSAvailable sinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
VRER variant
Plasmid#138561PurposeExpresses human codon-optimized SpCas9-VRER and blasticidin resistance: EFS promoter-VRER-NLS-FLAG-P2A-BSDDepositorInsertVRER
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationD1135V, G1218R, R1335E, T1337RPromoterEFSAvailable sinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_SNIPR_CD8alpha variant 3
Plasmid#188363PurposeLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant3-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factorDepositorInsertPGK_antiCD19_CD8alpha-variant3-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
NODALvar-matDYK
Plasmid#115260PurposeFor mammalian expression of a human NODAL splice varaint open reading frame with an internal DYK tagDepositorInsertNODAL (NODAL Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
NODALvar-matMYC
Plasmid#115249PurposeFor mammalian expression of a human NODAL splice variant open reading frame with an internal MYC tagDepositorInsertNODAL splice variant (NODAL Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
NODALvar-T7TS
Plasmid#115251PurposeFor in vitro transcription of a human NODAL splice variant open reading frame RNADepositorInsertNODAL splice variant (NODAL Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
-
pLV_Zika_NS1_Flag (Variant: W98G)
Plasmid#79641PurposeThird generation Self-inactivating lentivector expressing NS1 (Variant W98G) from Zika virusDepositorInsertNS1
UseLentiviralTagsFlagExpressionMammalianMutationVariant: W98GPromoterEF1Available sinceSept. 22, 2016AvailabilityAcademic Institutions and Nonprofits only