Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 1,091 results for: addgene

Showing: 301 - 320 of 1091 results

  1. Congratulations, Deck The Lab winners!

    Type
    Blog Post
    ...remotely instead of from the lab. Nonetheless, Addgene’s annual Deck the Lab competition drew upon the ...Decoration Competition! #ScienceFun #ZoomParty @Addgene pic.twitter.com/drNBUdlQco — The Tarling-Vallim...CVRHutchinson #SciArt #STEAM #WinterWonderlab @Addgene Make your own here!: https://t.co/AnltWfKuQg pic.twitter.com...description of all the ornaments. #DeckTheLab @Addgene pic.twitter.com/GsAlD6sjM6 — Dr. Anne A Madden ...thank you to everyone who entered. Keep an eye on Addgene’s social media throughout the year for more fun ...decorations in the lab, and one for home. The Addgenies voted, and selected three winners in each category...

  2. What's New in CRISPR - Winter 2018

    Type
    Blog Post
    ... a few of the new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR...adapted for detecting SNPs. Learn more from this Addgene blog post Read the paper in Science Find the...editing is now possible. Learn more from this Addgene blog post Read the paper in Nature Methods Find...a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in the next What...please let us know! Additional resources on the Addgene blog Download the CRISPR 101 eBook Find our CRISPR...posts Read our CRISPR cheat sheet Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids by...

  3. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...this year!  Find Addgene's own Lentiviral backbone plasmids here! Figure 2: Addgene cloned EGFP into...Lenti CMV N-HA Puro (Addgene #236079) to create pAG Lenti CMV HA-EGFP Puro (Addgene #236081), then generated...particles in HEK293T cells with pMD2.G (Addgene #12259) and psPAX2 (Addgene #12260). HeLa cells were infected...Anti-GFP [N86/38.1R] (Addgene #180084), 0.01 µg/mL Anti-HA [12CA5] - Chimeric (Addgene #198003)) and Goat...increased sensitivity and tailored deactivation rates Addgene's own empty lentiviral backbones New recombinant...2025.03.20.643984.  Build your way: Explore Addgene's new empty backbones By Meghan Rego You asked, ...EGFP-expressing lentiviral backbones designed right here at Addgene. Here's what makes these new backbones special:...

  4. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    ...began working remotely recently? Most of us at Addgene began working from home last week to flatten the...new. We usually have yoga class twice a week at Addgene HQ, but now we’re sharing links to online yoga ...before bed will help you prepare for sleep. At Addgene, our mindfulness group is sticking with their Headspace...virtually connected with the people in your life. At Addgene, we’ve started virtual activities like team lunches...societies hosting virtual networking events. Download Addgene’s Science Career guide to learn more about career...framework to design your future experiments.  Use Addgene’s AAV Data Hub. You can search the AAV Data Hub ... to use in future experiments. If you’ve used Addgene’s AAV tools, you can contribute data to the AAV ...

  5. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    ...As part of our mission, we’ve been working with Addgene to develop and apply our plexWell™ Library Preparation... for NGS-based sequencing and confirmation of Addgene’s large and growing collection of curated plasmids...are using this technique in our partnership with Addgene to sequence large numbers of plasmids. plexWell...does not vary by DNA input amount. plexWell™ + Addgene = high quality NGS-assembled plasmids Because of...original sample. At seqWell, we have worked with Addgene to develop a highly optimized bioinformatics pipeline...circularized plasmids from plexWell™ NGS data. Once Addgene produces plates of plasmid DNA, they are shipped...NGS-based assemblies for over 3000 plasmids in the Addgene collection.   Check out the SeqWell website to...

  6. Pooled Library Amplifications

    Type
    Blog Post
    ...Libraries Resources on Addgene.org Addgene's Guide to Plasmid Pooled Libraries  Addgene's Pooled Library Collection...contact us at deposit@addgene.org. Happy amplifying! More resources on the Addgene blog Genome-Wide Screening...If you’re depositing a pooled library with Addgene, you may be surprised to learn that we ask for an ...recombination and loss of plasmid diversity, so Addgene strives to distribute aliquots of the original ...requesting scientist receives a pooled library from Addgene, they’ll need to amplify the sample for further...generally cheaper and easier. One library that Addgene distributes with a chemical transformation amplification...Table 1: Examples of Different Pooled Libraries at Addgene.  Library Type Name Number of Plasmids ...

  7. Participate in the Pets of Addgene Scavenger Hunt

    Type
    Blog Post
    ...You will be trying to find pictures of Addgene pets with Addgene-branded clothing on the blog. Below each...just fun and games this April Fool’s Day on the Addgene blog.  We’ve been mostly working remotely for the.... Now, we’ve collected photos of our pets with Addgene gear, and have unleashed them all over our recently...

  8. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...plasmids from this publication at Addgene. Additional Resources on the Addgene Blog  Read more about Cpf1... will the number of reagents available through Addgene! If you're trying to decide which CRISPR tools .../nature24049 Find plasmids from this paper at Addgene. Amrani N, Gao XD, Liu P, Edraki A, Mir A, Ibraheim...science.aaq0180 Find plasmids from this paper at Addgene. Kim D, Kim J, Hur JK, Been KW, Yoon S, Kim J-...nbt.3609 Find plasmids from this publication at Addgene. Kim E, Koo T, Park SW, Kim D, Kim K, Cho H-Y,...ncomms14500 Find plasmids from this publication at Addgene. Kleinstiver BP, Prew MS, Tsai SQ, Topkar VV, ...nature14592 Find plasmids from this publication at Addgene. Kleinstiver BP, Tsai SQ, Prew MS, Nguyen NT, ...

  9. Biosensor AAV Preps

    Type
    Collection
    ...Ready-to-use AAV available from Addgene's viral service encoding biosensor tools, including calcium sensors...

  10. Supporting Rare Disease Research with BeHeard 2017

    Type
    Blog Post
    ...resources for those studying rare disease. This year, Addgene will be providing plasmid grants worth $2,000 each...BeHEARD Award. Read on to learn how plasmids from Addgene will be used to accelerate rare disease research...his work in C. elegans, Dr. Di Schiavi will use Addgene plasmids to characterize smn1 interactions with...plan to use CRISPR and lentiviral plasmids from Addgene to knockout, knockdown, or overexpress these candidates...underrepresented individuals deserve a voice. Check back with Addgene often to learn of future rare disease research ...19 (2015): S64-S65. Additional Resources on the Addgene Blog Previous BeHeard Awardees Other Efforts to...Developing Countries with Seeding Labs Resources on Addgene.org Find Plasmids for Disease Research ...

  11. Introducing...Addgene Quick Tips!

    Type
    Blog Post
    ...perhaps are a little limited on time — check out Addgene’s new Quick Tip series on YouTube! In this series...

  12. A Case Study of Addgene Bioinformatics

    Type
    Blog Post
    ...collaboration.  In that post, we talked about Addgene’s data processing needs, our criteria for selecting...Recently, Seqera published a case study on how Addgene has used Nextflow to improve and redesign our pipeline...

  13. Introducing Addgene's Microbiology Pages

    Type
    Blog Post
    ...Addgene will be at the American Society for Microbiology Microbe meeting here in Boston next week - be... and say hello! Just in time for this meeting, Addgene is launching a new resource page for the Microbiology.... coli and yeast, you’re in luck! Addgene has so many of these resources that we created filters just ...microbial technology, sign up to write about it on the Addgene blog. ...plasmids made in your lab, see www.addgene.org/deposit or email deposit@addgene.org for more information or ...organisms we should include by emailing us at help@addgene.org. If you are looking for plasmids used in E. ...

  14. 2022 and 2023: Reflections and Looking Forward

    Type
    Blog Post
    ...quietly learning about Addgene’s past and present, but the real promise of Addgene lies in our future. As...Addgene saw big changes in 2022, from the launch of a new material to the redesign of a community resource...organization. One thing that will remain the same is Addgene’s commitment to supporting the research community...number of people. I look forward to working with Addgene colleagues, collaborators, partners, and the larger...

  15. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    ...announce that the plasmid most requested from the Addgene repository in 2015 was... lentiCRISPR V2   LentiCRISPR...Availability and Use LentiCRISPR v2 is available from Addgene both as a single empty vector and in pooled libraries...reagents available to the research community through Addgene and look forward to another great year of sharing...PubMed Central PMCID: PMC4486245. Resources on the Addgene Blog Browse Our CRISPR Posts Learn about Using... Plasmids to Mammalian Cells Resources on the Addgene Website Visit our Lentiviral Guide Pages Find ...

  16. New Videos: Addgene Lab Tips

    Type
    Blog Post
    ...blog@addgene.org.  Additional Resources on the Addgene Blog New Educational Resource: The Addgene Videos...Addgene has distributed over 800,000 plasmids since 2004. In that time, our lab has gotten a lot experience...decided to share some of our tips in our new series: Addgene Lab Tips! In each short episode of this series...Science: We Share Science Additional Resources on Addgene.org Browse our Video Library Check out Our Protocol...

  17. Lab to Office Culture Shock

    Type
    Blog Post
    ...Resources at Addgene.org Find careers at Addgene Watch out career videos Sign up for the Addgene quarterly...making this transition. In this installment of the Addgene career guide, we’ll think about what causes this...job candidate for a senior scientist position at Addgene, the skeptical applicant asked if she would partake...of culture shock above, but after polling some Addgene colleagues, we’ve come up with a list of things...the years that one of the things I enjoy about Addgene is that I’ve never been bored. There is always ...someone who coordinates cross-team projects at Addgene, I find that my vocabulary improves on a weekly...fix them, and take a look at these tips from the Addgene blog. You’ll have the opportunity to discover...

  18. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    ...Introduction to Antibodies Resources on Addgene.org  Browse Addgene's recombinant antibody plasmid collection...Addgene has recently announced the launch of an open-access recombinant antibody resource called NABOR...expressed from plasmids that have been deposited with Addgene. Here, we take a closer look at the construction...that will be the first antibodies distributed by Addgene.  The plasmids used to generate these antibodies...sequence. These plasmids have been deposited with Addgene for distribution to the scientific community with...affinity reagent tools to include nanobodies. At Addgene, we are excited to be part of this initiative and...References and Resources Additional Resources on the Addgene blog:  Plasmid-Based Recombinant Antibodies  Plasmids...

  19. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

    Type
    Blog Post
    ... available at Addgene Read about SARS-CoV-2 detection methods Resources on Addgene.org Find all plasmids...been functionally tested by Ginkgo Bioworks or Addgene. If you used plasmids from this collection in your...cerevisiae expression vectors are available at Addgene. A set of mammalian expression vectors containing...COVID-19 Plasmid Collection Quality control at Addgene in the midst of COVID-19 We received Ginkgo’s first... our standard full plasmid sequencing by NGS. Addgene’s QC scientists immediately screen these plasmids...screening and the use of high throughput platforms, Addgene and Ginkgo are striking a balance by modifying ...978-1-59745-181-9_23 Additional resources on the Addgene blog Read all of our COVID-19 blog posts Learn...

  20. Genome Engineering, CRISPR, and Research in Singapore: Interview with Wei Leong Chew

    Type
    Blog Post
    ...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.      Additional...Additional Resources from the Addgene Blog Visit our CRISPR featured topic page Check out our Hot Plasmids... addgene podcast...synthetic biology researcher Tom Ellis Resources at Addgene.org Find CRISPR Plasmids Find Synthetic Biology ...
Showing: 301 - 320 of 1091 results