We narrowed to 398 results for: aga

1. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

   Type

   Blog Post

   ...with 10 sgRNAs in the sgRNA metrics, confirming again that sgRNA design plays a significant role in library...

2. AAVs in Retinal Gene Therapy

   Type

   Blog Post

   ...emerges from disgrace to be the next big thing, again 3. Acland G. et al., 2001. Gene therapy restores...

3. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

   Type

   Blog Post

   ...localization of BiFC constructs should also be compared against those of their endogenous counterparts (by immunocytochemistry...

4. A Guide to Starting Your Own Journal Club

   Type

   Blog Post

   ...the questions “Would you attend this journal club again?” and “How would you improve the structure of the...

5. SciComm: Taking Science to Elementary and Middle Schools

   Type

   Blog Post

   ...jobs, and an enthusiasm for coming back and trying again next year. There are many reasons why you might ...

6. Negative Can Be Positive: Open AAV Data with Addgene

   Type

   Blog Post

   ...D-F)  High-magnification images immuno-stained against dopamine beta-hydroxylase (DBH, magenta) to label...

7. An Introduction to Adenovirus

   Type

   Blog Post

   ... Vectors Overcome Preexisting Humoral Immunity against Human Adenoviruses In Vivo. J Virol, 73(8), 6930...

8. Viral Vectors 101: The Retroviral Lifecycle

   Type

   Blog Post

   ...emerges. Retrovirus life cycle Retroviruses go against the grain of the Central Dogma of Biology (gDNA...

9. Great Results Start with Great Standard Curves

   Type

   Blog Post

   ...concentration of a serially diluted standard is plotted against its OD560 measurements and the trendline y = 0.6561x...

10. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type

    Blog Post

    ...plasmid from the NeuroMab collection for an antibody against PARIS, a zinc finger protein involved in neurodegeneration...

11. Simplify Cloning with in vivo Assembly

    Type

    Blog Post

    ...vitro, before transforming it into E. coli for propagation. However, while not widely known, plasmid assembly...

12. Lentiviral Vector Uses and Overview

    Type

    Blog Post

    ...lentiviral vector containing an antisense sequence against the HIV-1 envelope. As of 2013, 65 patients had...

13. Plasmids 101: Colony PCR

    Type

    Blog Post

    ... complete, it’s time to run the products on an agarose gel to determine their size. Make sure to use an...

14. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type

    Blog Post

    ...separate plasmids), there are a few reasons arguing against this approach. First, this would prevent the study...

15. Tips for Screening with Yeast Two Hybrid Systems

    Type

    Blog Post

    ... possible to screen libraries of bait and prey against each other at the same time in order to try and...

16. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type

    Blog Post

    ...track your samples today and never lose a sample again!   Amanda Hazen is the Laboratory Operations Manager...

17. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type

    Blog Post

    ...genome functions in the hope of developing drugs against intractable diseases. Currently, a number of labs...

18. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type

    Blog Post

    ...molecular cloning protocols, including making LB agar plates, restriction digest of plasmid DNA, and bacterial...

19. Negotiating Work and Life: How to Find the Joy

    Type

    Blog Post

    ...children, so my life will be under control.” Think again. Maybe you’ll need to care for an elderly family...

20. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type

    Blog Post

    ... Barrangou R, Boyaval P, Fremaux C, Horvath P, Magadán AH, Moineau S (2010) The CRISPR/Cas bacterial immune...