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We narrowed to 581 results for: arl

Showing: 301 - 320 of 581 results

  1. Evolution of Lab Techniques

    Type
    Blog Post
    ...cars become more technologically advanced leaving earlier versions obsolete, the techniques we use in lab...researchers at a time when sequencing was still in the early days of development. Today, researchers use both...

  2. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ...this way, if you know what you need. Most often, early career scientists aren’t trained to have these types...based on financial values. All too often, we see early investigators making unnecessary short term sacrifices...

  3. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...unanticipated sites. While off-target effects are nearly impossible to eliminate, it’s essential to understand...appropriate method for an activator or repressor Cas. Similarly, Cas ChIP-Seq would also identify all DNA binding...

  4. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...different system (Issa et al., 2023). This can be particularly burdensome in the brain where many different...such as chicken-beta-actin or CAG can drive strong early gene expression, but can become methylated over ...

  5. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...requires a nearby PAM, although these are still early days for this technology, and the user may need ... P-Y, Daley G, Church G (2013) Optimization of scarless human stem cell genome editing. Nucleic Acids ...

  6. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...specific changes into the mouse genome. With these early technologies, a researcher would first introduce... is a molecular biologist with Biogen and is particularly interested in applying CRISPR for mouse genome...

  7. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ...to recognize DNA sequences is not ideal. For the early generation gene editors, new amino acid recognition...engineering of novel base editor proteins. She is particularly interested in combining the realms of art and...

  8. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...waivers. One of the blog posts that helped me quite early on was Christine Liu’s “How to Become a Scientist...on the Addgene blog Check out our resources for early career researchers Get advice for applying for jobs...

  9. Antibodies 101: Validation

    Type
    Blog Post
    ... does not mean that is all it will bind to - particularly when you start using complex samples such as... the antibody webpages. These outlets can be particularly useful for sharing negative data. Negative data...

  10. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...bacteria or cell-free systems. The approach is particularly useful for producing drug precursors, biofuels...works in both mammalian systems and Drosophila. Similarly, the Multi-Kingdom (MK) Golden Gate system was...

  11. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ... different colors. This lends flow cytometry particularly well to complex experiments looking at a variety...from the use of secondary antibodies. It’s also particularly useful for intracellular staining where binding...

  12. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...Initial photoactivatable CRISPR systems published in early 2015 focused on using light to control transcription...Moritoshi Sato’s lab at the University of Tokyo and Charles Gersbach’s lab at Duke University developed similar...

  13. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...Early CRISPR applications were often limited by the low editing efficiency of homology-directed repair...nickase (Nishida et al., 2016). Target-AID acts similarly but not identically to BE3, modifying a 3-5 base...

  14. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...enough to get good separation in your ladder, particularly in the sizes around your protein of interest... leftover sample) to fill the relevant wells. Similarly, if you load a gel and then forget to start it...

  15. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...provides improvements to classical cloning methods, particularly for complex transgenesis constructs. Kirchmaier...terminator). Next, up to six Level 1 modules can be similarly assembled into a Level 2 vector, thus forming ...

  16. Chemogenetics Guide

    Type
    Guide
    ...Chemogenetics Guide Jump To: Chemogenetics Guide Early Chemogenetic Receptors: RASSLs DREADDs PSAMs LMOs...General overview of a chemogenetics experiment. Early Chemogenetic Receptors: RASSLs The first chemogenetic...

  17. Optogenetics Guide

    Type
    Guide
    ...and light oxygen voltage (LOV) domains function similarly to cryptochromes with light-induced changes in...Synthetic Neurobiology Group Optogenetics Resources Karl Deisseroth's Lab: Optogenetics Resource Center Boyden...

  18. Molecular Biology Reference

    Type
    Guide
    ...of phenotypic information was introduced in the early 19th century by Gregor Mendel, who later demonstrated...clones of the original cell. The plasmid DNA is similarly referred to as cloned DNA, and this process of...

  19. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...viral genome. Helper plasmid E4 in trans Adenoviral early gene that encodes for proteins essential for AAV...replication and packaging. E2A in trans Adenoviral early gene that encodes a DNA-binding protein (DBP) that...

  20. Antibodies 101 Series on TikTok

    Type
    Blog Post
    ... Addgene We'll be posting these animations regularly from now until May, so follow us to get updates...
Showing: 301 - 320 of 581 results