We narrowed to 648 results for: ato
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TypeBlog Post...experiments in the beginning. In most classrooms, the laboratory experience is focused on classical embryology...for other developmental models including flies, nematodes, and zebrafish Find plasmids for Mammalian RNAi...
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Pathways Over Time Plasmids Engage Students in Functional Genomics Research
TypeBlog Post...experiences to undergraduate students (1, 2), but educators face significant challenges in designing suitable...Functional genomics research in an introductory laboratory course. CBE-Life Sci. Educ. 17, ar1. (2018). ... -
Open Resources and Plasmid Tools For Studying C. elegans
TypeBlog Post...open worm browser, which allows you to view the anatomy of worm layer by layer, the NeuroML C. elegans ...neural network, and Sibernetic, a fluid mechanics simulator that can be used to simulate muscle tissue as ... -
Corralling and Cornering the Goldilocks Antibody
TypeBlog Post...high-quality antibodies for IHC. Working with collaborators and support from the Brain Research Through ...intuitive guide for checking their staining and neuroanatomy without the fear of referencing a wrong section... -
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation
TypeBlog Post...Daniel M. Sapozhnikov, a graduate student in the laboratory of Dr. Moshe Szyf at McGill University. Some ...Daniel M. Sapozhnikov is a graduate student in the laboratory of Dr. Moshe Szyf at McGill University, working... -
Fluorescent Proteins 101: Introduction to FRET
TypeBlog Post...our curated list of biosensors too, as another laboratory may have already created the sensor that you ...PMC18018. 2. Miyawaki, Atsushi, et al. "Fluorescent indicators for Ca2+ based on green fluorescent proteins ... -
Quickest Way to Deposit Plasmids: The Deposit Spreadsheet
TypeBlog Post...zebrafish), D. melanogaster (fly), C. elegans (nematode), S. cerevisiae (budding yeast), S. pombe (fission...Distribute pending QC. The name of the Principal Investigator and Organization where your plasmids were first... -
Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene
TypeBlog Post...ask the depositing laboratory to review the discrepancies. If the depositing laboratory confirms that these... -
Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015
TypeBlog Post...human tRNA genes and, until now, we've overlooked regulatory fragments of these transcripts. I remember when...Medicine presented another unexpected finding in the regulatory RNA field. The Rajewsky lab is studying circular... -
Polymerase Chain Reaction Overview and Applications
TypeBlog Post...If you have ever worked in a molecular biology laboratory you have likely done a polymerase chain reaction...method that can help save time and effort in the laboratory. There are two main categories of multiplex ... -
Fluorescent Tagging of Endogenous Genes with SapTrap
TypeBlog Post...additional protein characteristics. For example, Masato Kanemaki's lab has developed a CRISPR/Cas-based...of auxin to the culture medium. Any number of regulatory sequences, including protein enhancers and repressors... -
Quick Guide to Working with Drosophila Part 1: Getting Started with Flies
TypeBlog Post...daytime. They mostly stop emerging at night. An incubator with a light cycle makes for very predictable ... phenotypes, check out the Classical Genetics Simulator website. One interesting thing to know is that... -
Pooled Library Amplifications
TypeBlog Post...protocol, the size of the cuvettes your lab’s electroporator uses, etc. The DNA amount can be anywhere from...Screening Zhang Lab - Multiplexed Overexpression of Regulatory Factors (MORF) Library 3,548 250 - 10,000... -
Experimenting with New Careers while in Grad School
TypeBlog Post...in real time! I follow great online science communicators like Phil Plait @BadAstronomer, Emily Graslie...when I was trying to find local informal science educators, and ever since, the State Museum has been the... -
New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries
TypeBlog Post...CRISPR/Cas9 Synergistic Activation Mediator) uses an improved activator design that consists of inactive... -
AAVs in Retinal Gene Therapy
TypeBlog Post...viral vectors) without triggering a destructive inflammatory immune response. At the time, adenoviral vectors...choroideremia, X-linked retinitis pigmentosa, achromatopsia, and age-related macular degeneration are ongoing... -
The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data
TypeBlog Post...University; and Howard Hughes Medical Institute Investigator. Liu’s research integrates chemistry and evolution...four examples of technologies pioneered in his laboratory. Learn more here. Additional resources on the... -
What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry
TypeBlog Post...(Figure 1B). The A260/230 ratio can be a good indicator of protein contamination, but a number of other...researchers traditionally use the A260/280 ratio as an indicator of protein contamination. However, this ratio ... -
Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus
TypeBlog Post...Microbiology, National Emerging Infectious Disease Laboratories. She is interested in genome replication and...Karl-Klaus Conzelmann. "Generation of bovine respiratory syncytial virus (BRSV) from cDNA: BRSV NS2 is... -
The Importance of a Fun Workplace: Company Culture at Addgene
TypeBlog Post... confident that their teammates and project collaborators have completed their work thoroughly. It’s kind...Veckerelli is the Administrative and Operations Coordinator at Addgene. In addition to her operational responsibilities...