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1. Truncated gRNAs for Regulating Gene Expression

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   Blog Post

   ...binding the protospacer. The resulting double strand break (DSB) stimulates DNA repair mechanisms that can ...

2. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

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   ... Vlieger D, Corbett KS, Torres GM, Wang N, Van Breedam W, Roose K, van Schie L, Hoffmann M, Pöhlmann S...

3. Polymerase Chain Reaction Overview and Applications

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   ...dsDNA template to a point where the hydrogen bonds break between the base pairs. This results in the separation...

4. Degrading DNA with Cascade-Cas3

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   ...During DNA repair, there is a chance that the two breakpoints will repair without the section between the two...

5. Common Injection Routes in Mice

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   ...patience, as you wait for the anesthetized mice to breathe in the liquid. Restraint: anesthesia Location: ...

6. Twenty Years of Addgene Sharing: CRISPR

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   ...effectors. Nucleases generate a classic double-stranded break, while modulators regulate transcription and chimeric...

7. PhD Applications After COVID

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   ...and video communication. But first, take a deep breath; at least you know you have an answer to that previous...

8. 3D Printing Meets CRISPR Cas9

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   ...sequence of events plays itself out: The researcher breaks eye-contact with any other human in the room. Their...

9. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

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   .... However, if the molecules are confined to a subregion of the cell and/or they cannot tumble in 720°,...

10. Experimenting with New Careers while in Grad School

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    ...to get recharged. The fun change of pace helped break up the negativity and allowed me to focus on the...

11. Genome-wide Screening Using CRISPR

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    ...-type Cas9 and a gRNA generates a double-strand break in the target DNA, which, when repaired through ...

12. Light Sheet Fluorescence Microscopy

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    ... the human ubiquitin C promoter. Anatomical abbreviations: Gr= Gracile tract, Cu= Cuneate tract, dCST=...

13. Which Fluorescent Protein Should I Use?

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    ...CFP), and yellow FP (YFP) were derived. For a breakdown of GFP, it's variants, and their relevant mutations...

14. CRISPR 101: Ribonucleoprotein (RNP) Delivery

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    ...Zhen, et al. "Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes...

15. AAVs in Retinal Gene Therapy

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    .... Jean Bennett (UPenn) that resulted in the groundbreaking 2001 study showing that AAV-mediated delivery...

16. Transferable Skills Guide: Identifying Your Transferable Skills

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    ...employer. Your heart is pounding, you’re short of breath, and you’re a little jumpy. You are at another ...

17. Viral Vectors 101: The Retroviral Lifecycle

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    ...tools in molecular biology. “Retroviruses” is an umbrella term which encompasses 1.) the gamma-retroviruses...

18. Getting to Know Expansion Microscopy

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    ...SRM), a family of imaging techniques developed to break through the resolution barrier while still utilizing...

19. Great Results Start with Great Standard Curves

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    ...and the quality of a standard curve can make or break an experiment. Here, we will provide an overview...

20. DNA Purification Without a Kit

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    ...method for you! And even if you’re not ready to break-up with your DNA purification kit, you can still...