We narrowed to 304 results for: cas9 genes

...articles, genes, and blog posts are not comprehensive, so just because you do not see a name or gene on this...plasmids containing these genes. As with the PIs above, many of these genes were discussed at the 2018 Keystone...HIST2H2BE RBX1 STAG2 Xist Your favorite gene! SWI/SNF Genes:       BRG1(SMARCA4) BAF170 (SMARCC2...Interactions: Using CRISPR-dCas9 to Study Gene Regulation CRISPR 101: Epigenetics and Editing the Epigenome...You!     Chromatin-related genes with plasmids available from Addgene The links below pull up lists...containing the indicated gene, and you can subscribe to these pages to learn when Addgene receives new plasmids... In-exhaustive Chromatin-related genes with plasmids on Addgene.org AGO1 BRCA1 CREBBP CUL1 H2AFX ...

...on vectors where the gene of interest is inserted into the middle of the lacZ gene, thus disrupting 𝛽-...where only cells that have gained or lost a specific gene survive (ex: antibiotic resistance marker). In a... the blue-white screen, which relies on the lacZ gene. lacZ encodes the enzyme 𝛽-galactosidase which ...scientists have found that when a portion of the gene is deleted, it creates a non-functional 𝛽-galactosidase...mentioned above. In molecular biology, cloning a gene into your plasmid is often a numbers game. By using...troubleshooting Sanger sequencing results. At Addgene, we now use next-generation sequencing during our QC process....comes in. Let’s take a look at All_in_one_CRISPR/Cas9_LacZ, a CRISPR gRNA plasmid from Lynne Postovit’s lab...

...which NANOG-related genes are critical to stem cell survival. Knowing which genes influence the survival...compaction, an enhancer can increase the expression of a gene that is millions of base pairs away. (For details...GPS for the genome” to compute coordinates for genetic elements like protein, RNA, and DNA (Table 1). ...family as HIV. By design, our lentivirii are genetically programmed to CRISPR out the enhancers we identified...differentiate over time, we periodically use next-generation sequencing to measure the relative proportion...pillar and the ESC dies. Order Lentivirus from Addgene If you’re interested in designing your own CRISPR...Hypersensitive Sites and Intergenic CRISPRs Yours truly generated the DHS data set by starting with all of the DHS...

...Community Addgene: In 2012 your lab first deposited plasmids with Addgene. In 2014, you joined Addgene's Advisory...lab. In June 2014, Dr. Fujii joined Addgene's Advisory Board.  Addgene: Your lab has worked extensively ... enChIP through Addgene. Retroviral Expression System for enChIP using CRISPR Addgene: Your lab recently...applications? Fujii: We generated a series of retroviral vectors expressing tagged dCas9 and guide RNA. The...the plasmids of these systems to Addgene in the near future. Addgene: Has your lab been working on any...first knew Addgene around 2010 and found the scheme very nice at first sight in that Addgene takes care...ourselves without Addgene. So, when I received an invitation to join the Advisory Board of Addgene, I accepted...