We narrowed to 422 results for: cript

...cause off-target effects in a variety of RNA transcripts independent of Cas9 targeting (Rees et al., 2019...

...fact! Endogenous miRNA production starts with transcription of the miRNA to create pri-miRNA, which is then...

...encoding your repair template, then uses reverse transcriptase to generate the complementary ssDNA. Easi-CRISPR...

...together a video from their recorded answers. No scripting made this a lot easier and I didn’t need to be...

...prevails. Sequencing whole genomes or profiling transcriptomes (RNAseq) require sequencing of many samples...

...PI on the panel even suggested projecting your manuscript onto a wall and reading it out loud. While it...

...addition of a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), commonly used to...

...mutations found in E.coli strains Gene(s) Description Functional Consequence dam DNA adenine methylase...

... insertion, a combined endonuclease-reverse transcriptase uses homology arms flanking an RNA payload to...

...and probe with live cells The following is a description of a technique published by Simpson, Sacher, ...

...effects, such as with signaling molecules or transcription factors, or when the gene is constitutively-...

...and can immediately undergo replication and transcription. However, with scAAV the effective genome size...

...transduced by a GFP encoded virus, and the reverse transcriptase activity assay uses qPCR to quantify viral RNA...

...members could be recruited, but certainly not conscripted. There’s also no end-game here, unless we manage...

...levels to generate anything from individual transcription units (TUs) used to express your favorite gene-of-interest...

...fluorescent donors and acceptors in their product descriptions, or technical manuals. Specific applications...

...transdifferentiation is usually carried out by expressing transcription factors specific to the lineage of the target...

...commercially available. Various proteomic and transcriptomic datasets can be used to select a candidate ...

...Sendai virus doesn’t rely on promoters for transcriptional regulation, so it requires a different approach...

... The human U6 promoter prefers a 'G' at the transcription start site to have high expression, so adding...