We narrowed to 88 results for: cript

...VP16 transcriptional activation domain). For simplicity, other promoter elements and transcription machinery...Plasmid Description PI 63800 PB-TRE-dCas9-VPR Tet-inducible expression of SP-dCas9-VPR transcriptional activator..., the TetR protein binds to tet O, blocking transcription of the downstream gene. If tet or one of its...to tetracycline and releases tet O, enabling transcription (Figure 1). While TetR and tet O could be a ...tetracycline-controlled transactivator (fusion of TetR with VP16 transcriptional activation domain); rtTA: reverse tetracycline-controlled...to eliminate binding of endogenous mammalian transcription factors to further minimize background expression...plasmids use "TetO" or "Tet-O" in their name or description, meaning they contain a TRE or tet O and can ...

... PI Description MORF library 137000, 192821, 1000000218 Zhang A collection of human transcription factor...Browse the Zhang lab’s comprehensive human transcription factor collection, available at Addgene. The...The collection can be used for screening transcription factors for cellular programming or cancer drug ...Library The MORF library is a comprehensive human transcription factor collection that contains 1,836 genes ...collection, can be purchased at Addgene. Each transcription factor isoform has a unique 24 bp barcode that...that simplifies identification of the transcription factor isoform in pooled screens. This collection ...collection and library can be used for screening transcription factors for applications such as cellular programming...

...Targeting dCas9 to transcription start sites is sufficient to repress transcription by blocking initiation...approaches like zinc finger nucleases (ZFNs) or transcription-activator-like effector nucleases (TALENs) required...which Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) is fused to the C-terminus of Cas9...edit is incorporated into the DNA by reverse transcription. The edited DNA strand displaces the unedited...uses a CRISPR-Cas9n fused to both a reverse-transcriptase and a serine integrase (Bxb1), along with two...initiation. More robust transcriptional repression (CRISPR interference, or CRISPRi) or activation (CRISPRa...target genes can be achieved by fusing dCas9 to transcriptional repressors or activators and targeting promoter...

...Empty Backbones Plasmid Description Industry Return to top Blue Plasmid Description Industry Return to top...top Cyan Plasmid Description Industry Return to top Green Plasmid Description Industry Return to top mEmerald... mEmerald Plasmid Description Industry Return to top Yellow Plasmid Description Industry Return to top...top Orange Plasmid Description Industry Return to top Red Plasmid Description Industry Return to top Photoactivatable...photoactivatable fluorescent proteins. Plasmid Description Industry Return to top Content last reviewed:...

... protein half-lives, making them useful as transcriptional reporters, but are less-than-ideal protein ...mammalian systems. ID Plasmid Luciferase Type(s) Description PI 64784 pGL3-Basic-IRES Firefly Insertion of...Vector for investigating regions controlling transcription Debrya Groskreutz 18760 MSCV IRES Luciferase...Vector for investigating regions controlling transcription Pete Stecha 212933 pGL4.82(hRluc/Puro) Renilla...Vector for investigating regions controlling transcription Pete Stecha 106292 pLS-SV40-mP-Rluc Renilla ...plasmids. ID Plasmid Luciferase Type Promoter Description PI 60226 AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc...pEGB 35S:Luciferase:Tnos (GB0110) Firefly 35S Transcriptional unit for firefly luciferase expression in plants...

...at the bottom of this page. For more in depth descriptions of each serotype, check out Addgene's blog on...Browse Available PHP.eB AAV ID Name Promoter Description Category PI Controls 28306 pAAV-FLEX-tdTomato... Browse Available PHP.S AAV ID Name Promoter Description Category PI 28306 pAAV-FLEX-tdTomato CAG tdTomato...Browse Available PHP.V1 AAV ID Name Promoter Description Category PI 104052 pAAV-CAG-DIO-EYFP CAG EYFP...Browse Available MaCPNS1 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control...Browse Available MaCPNS2 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control...Browse Available CAP-B10 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control...

...find more information. Delivery Method Species Description Article PI Lentivirus Human Expression of human...human Klf4, Oct4, c-Myc, and Sox2 as VP-16 transcriptional activating fusions in single lentiviral vectors...human iPSC generation OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming Human Fibroblasts...Progenitor Cells Lentiviral Human Cooperative Transcription Factor Induction Mediates Hemogenic Reprogramming...Oligodendrocyte Progenitor Cells Lentiviral Mouse Transcription factor-mediated reprogramming of fibroblasts...fibroblasts into functional astrocytes by defined transcription factors. Stem Cell Reports. 2015 Jan 13;4(1)...Neurons Lentiviral Human Chemical modulation of transcriptionally enriched signaling pathways to optimize the...

... interfere with transcription elongation. CRISPR Activation : Target the transcription start site. CRISPRa...fused to transcriptional activators that need to be within a given range of the transcription start site...Prime editor (Cas9 nickase fused to reverse transcriptase (RT)) Can edit within a wider window than most...genome sequence dCas9 or dCas9 fusion with transcriptional repressor (such as dCas9-KRAB) dCas9-KRAB can...modifying the genome sequence dCas9 fusion with transcriptional activator (such as dCas9-VP64) Many different... interest, where the Cas9 protein can block transcription from ever initiating. gRNAs targeting regions...and gRNA. This method involves using in vitro transcription reactions to generate mature Cas mRNA and gRNA...

...Polymerases Restriction Endonucleases Reverse Transcriptases RNA Polymerases Other Enzymes Additional Resources...polymerases, RNA polymerases, DNA ligases, reverse transcriptases, and restriction enzymes. These enzymes are... cloning, isothermal amplification, reverse transcription of RNA into DNA, and dNTP synthesis. Additionally...Polymerases Restriction Endonucleases Reverse Transcriptases RNA Polymerases Other Enzymes DNA Ligases Addgene...165568 pOpen-NcoIR NcoI Return to top Reverse Transcriptases Addgene ID Plasmid Gene/insert 165546 pOpen-MMLV_RT...Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase (RNAse H deactivated by 3 mutations) 165556 ...Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase RNaseH - (lacking RNaseH domain) 165566 pOpen-TthRT...

... ID Plasmid Depositing PI Description Golden Gate TALEN 2.0 38142 RCIscript-GoldyTALEN Daniel Carlson ...new tools for genome engineering. Fusions of transcription activator-like (TAL) effectors of plant pathogenic...with custom repeat arrays. TALEN Kits TALEN Kit Description Depositing PI FusX TALEN Assembly System Contains...expression of TALENs from a truncated CAGs promoter. RCIscript-GoldyTALEN is designed for in vitro synthesis ...strong nuclear localisation tags and repress transcription initiation when targeted to DNA sequences within...destination vectors can be used to create TALE-transcription factors and are used at the final assembly step...for generating TALEN mRNAs through in vitro transcription as an alternative. Based on transient double-selection...

...light”): Cre Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional Addgene resources...Return to top Flp Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional Addgene resources...Return to top Dre Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional Addgene resources...Site-Specific Recombinase Plasmids ID Plasmid Description Gene/Insert Vector Type Promoter PI Return to...target site (lox, FRT, rox, etc.) ID Plasmid Description Vector Type PI Additional Addgene resources Use...clones, and making transgenic mice. ID Plasmid Description Gene/Insert Vector Type PI Pooled Libraries Pooled... Pooled Library Name Type PI Description Mouse Tumor Suppressor Gene CRISPR Knockout Library CRISPR Sidi...

... bind transcriptional co-activators and modulate transcription. The list of p53 transcriptional targets...collection of plasmids for the p53 pathway. p53 is a transcription factor and tumor suppressor activated by cellular...p53 Gene List References Background p53 is a transcription factor and tumor suppressor activated by cellular...mutations interfere with p53’s ability to activate transcription, and they also have a dominant negative effect... receptor superfamily, member 10b E2F-1 E2F transcription factor 1 Fas Fas cell surface death receptor...

...promoter or cloning method of choice. ID Plasmid Description Promoter Cloning Method PI Synthetic Biology ...for creating plant expression vectors. ID Kit Description Cloning Type PI 1000000036 GreenGate Cloning ...different functional variants of Cas9. ID Plasmid Description PI Genome Editing Kits The following plasmid ...designed for genome editing in plants. ID Kit Description Gene Editing Type PI 1000000076 GoldenBraid 2.0...silencing suppressors, such as p19. ID Plasmid Description PI Additional Gene Silencing Resources A modular...physiological processes in plants. ID Plasmid Description PI Kits for Protein–Protein Interactions The ...protein–protein interactions in plants. ID Kit Description Cloning Type PI 1000000147, 1000000148, 1000000149...

...scientists. Antibodies ID Recombinant Antibody Description PI 211756 Anti-SARS-CoV-2 Nucleocapsid Protein...Plasmid Distribution to Industry page. ID Plasmid Description Industry PI Additional Addgene resources SARS-CoV... SARS-CoV, MERS, BAT-CoV Plasmids ID Plasmid Description Industry PI Due to export control regulations...plasmids related to these genes below. ID Plasmid Description Industry PI Additional Addgene resources ACE2... protease (Mpro) activity reporters Reverse transcriptase, protease, and polymerase constructs for SARS-CoV...Neutralizing nanobody plasmids And more! ID Plasmid Description Industry PI Addgene blog Finding nucleic acids...Pooled Libraries Pooled Library Name Type PI Description SARS-CoV-2 Spike (S) Ectodomain and RBD Libraries...

...IGDCC3 (PUNC) Antibodies ID Recombinant Antibody Description Isotype Reactivity Recommended Applications PI...scientists worldwide. ID Recombinant Antibody Description Isotype Reactivity Recommended Applications PI...Neuroligin Antibodies ID Recombinant Antibody Description Isotype Reactivity Recommended Applications PI... Integrin Antibodies ID Recombinant Antibody Description Isotype Reactivity Recommended Applications Return...common cellular proteins. ID Recombinant Antibody Description Isotype Reactivity Recommended Applications Return...express plasmid-based antibodies. ID Plasmid Description Return to top...

...disease severity. The MECP2 protein is a global transcriptional regulator of thousands of genes and studies...studies have suggested roles in transcriptional repression, activity dependent de-repression, chromatin remodeling...DNA and recruit known co-repressors or other transcriptional modifiers suggests the major function of MECP2...NTD) the M ethyl B inding D omain (MBD) a T ranscriptional R epressor D omain (TRD) the TRD contains a...species (mouse or human) Mutation ID Plasmid Description Gene/Insert Mutations PI Return to top External...Developing Human Brain - includes developmental transcriptome information, prenatal LMD microarray data, ISH...

... the REAL-Fast assembly method (Reyon et al., manuscript submitted), and the FLASH (Fast Ligation-based...Hockemeyer et al., Nat Biotechnol. 2011 ) TALE transcriptional activators in human cells ( Maeder et al., ...identify both dimeric TALEN and monomeric TALE transcriptional activator target sites and to simplify the ...sequences of interest for potential TALEN or TALE transcriptional activator target sites and then generates individual...Vectors for REAL, REAL-Fast and FLASH TALE Transcriptional Activator Expression Vectors for REAL, REAL-Fast...

...encoded on RNA. The genome includes a reverse transcriptase and an integrase to insert the viral genome ...plasmids that have inserts. ID Plasmid 5'LTR Description PI 1780 pBABE-neo largeTcDNA MoMLV Expression...plasmid 12282 for the addition of WRE, a post-transcriptional regulatory element that increases export to...Gamma-Retroviral Packaging Plasmids ID Plasmid Description PI 8449 pUMVC Contains MoMLV gag and pol . Use...Gamma-Retroviral Envelope Plasmids ID Plasmid Description PI 1733 pCI-VSVG VSV-G recommended for the FELIX...

...124606 SOX2-mEGFP AICSDP-60 mEGFP Transcription factor SOX-2 Transcription Factor 124607 ACTN2-mEGFP AICSDP...human cell lines of their choice. For a full description of how to use these plasmids, check out Plasmids...133963 UBTF-HaloTag AICSDP-80 HaloTag Nucleolar transcription factor UBF Nucleolus (fibrillar center) 133964...159745 TFAM-mEGFP AICSDP-72 mEGFP Mitochondrial transcription factor A (TFAM) Mitochondrial nucleoids 164499...

...example, search "Cdc42", "transcription", or other terms. ID Plasmid Description Gene/Insert Vector Type...many different types of proteins. For a full description (including peak response spectra) of different...preparations of many optogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter Eligible for...