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Showing: 301 - 320 of 631 results

  1. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ... a destination vector via Golden Gate cloning, perform an exonuclease cleanup, transform, plate, and pick...sequencing and found 98% of the plasmids were perfectly correct. Because they created the assemblies in...

  2. Hot Plasmids February 2024

    Type
    Blog Post
    ...quickly saw wide interest, its dimeric nature can interfere with some applications. Several labs have been... (Chuapoco et al., 2023). They found the best-performing variant, CAP-Mac, was effective at breaching ...

  4. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...embryonic genes are individually depleted via RNA interference (RNAi). Scientists monitor the effects of RNAi...RNAi using four-dimensional contrast interference (DIC) microscopy and computer imaging processing. The...

  5. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...non-target proteins), variability (separate batches can perform differently – happens when the antibody is produced...binding to off-target molecules), reliable (wouldn’t perform differently over time), compatible with the desired...

  6. How to Prepare for an Industry Interview

    Type
    Blog Post
    ...general questions about job responsibilities, performance evaluations, professional development, and company... research is a deliverable. Find 10 steps to a perfect science talk Be prepared for potential technical...

  7. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...their genes of interest. While recombinases are powerful gene expression tools in and of themselves, their...reduced scale in screens without any reduction in performance compared to larger genome-wide libraries. Find...

  8. Engaging with science and society at pgEd

    Type
    Blog Post
    ...policy requires broad interest in issues at the interface of science and society, and an ability to talk...in the legal, ethical and policy issues at the interface of biology and society.     Additional resources...

  9. 6 Tips for Grant Writing

    Type
    Blog Post
    ...’t common to non-scientists and may make your perfectly reasonable paragraph unintelligible to a lay audience...Science Communication Skills 5. Practice makes perfect, apply for small grants too There are actually ...

  10. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...target sites that flox your genes of interest, a powerful FLEx switch can be created for your specifc experimental...more information. References: 1. Schnütgen F, Doerflinger N, Calléja C, Wendling O, Chambon P, and Ghyselinck...

  12. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...through Cas3. After these initial steps, Cas3 can perform its helicase and nuclease functions simultaneously...likes to bluff, making you into think it’s more powerful than it is. The nuclease domain of Cas3 is considered...

  13. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...allows for a wide variety of screens, including interference, activation, and knockout. Most CRISPR screens...CRISPR Delivery Genome-wide Screening Using CRISPR Performing In Vivo CRISPR Screens Using the FITS Approach...

  14. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...just be an artifact and eliminate sources of interference, but the result persisted. (Even better, multiple...Although a ~1% change in signal might not make a very powerful tool, it was promising enough to keep exploring...

  15. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...student at the Max Planck Institute of Colloids and Interfaces. Here are some tips for cell culture that will...lab at the Max Planck Institute of Colloids and Interfaces. Sana is particularly interested in Glycobiology...

  16. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    ... NGA is a secondary PAM for SpCas9. Hu et al. performed GUIDE-seq analysis to characterize genome-wide...an NGG PAM, xCas9 3.7-VPR constructs modestly outperformed SpCas9-VPR in activating transcription. Sites...

  17. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    ...them. For example, gene knockdown with CRISPR interference (CRISPRi) has been limited to lab-adapted bacteria... Resources on Addgene.org Browse our CRISPR interference plasmids Find bacterial CRISPR plasmids Read...

  18. Of Myc and Men

    Type
    Blog Post
    ...antibodies (absolutely perfect in every way)? Unfortunately, no antibody is that perfect. One of the known ...

  19. PhD Applications After COVID

    Type
    Blog Post
    ...program you didn’t know much about could be your perfect fit! Even if you had planned a campus visit and...like sickness or scheduling issues can and do interfere with those in-person plans. Virtual options are...

  20. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...protein. The Bender’s popularity was short-lived as powerful molecular graphics packages were soon developed...channel.   He then said “I hadn’t realized how perfectly flat the complex is on this surface”….where the...
Showing: 301 - 320 of 631 results