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We narrowed to 636 results for: erf

Showing: 301 - 320 of 636 results

  2. 6 Tips for Grant Writing

    Type
    Blog Post
    ...’t common to non-scientists and may make your perfectly reasonable paragraph unintelligible to a lay audience...Science Communication Skills 5. Practice makes perfect, apply for small grants too There are actually ...

  3. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...target sites that flox your genes of interest, a powerful FLEx switch can be created for your specifc experimental...more information. References: 1. Schnütgen F, Doerflinger N, Calléja C, Wendling O, Chambon P, and Ghyselinck...

  5. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    ...them. For example, gene knockdown with CRISPR interference (CRISPRi) has been limited to lab-adapted bacteria... Resources on Addgene.org Browse our CRISPR interference plasmids Find bacterial CRISPR plasmids Read...

  6. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...just be an artifact and eliminate sources of interference, but the result persisted. (Even better, multiple...Although a ~1% change in signal might not make a very powerful tool, it was promising enough to keep exploring...

  7. Of Myc and Men

    Type
    Blog Post
    ...antibodies (absolutely perfect in every way)? Unfortunately, no antibody is that perfect. One of the known ...

  8. PhD Applications After COVID

    Type
    Blog Post
    ...program you didn’t know much about could be your perfect fit! Even if you had planned a campus visit and...like sickness or scheduling issues can and do interfere with those in-person plans. Virtual options are...

  9. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...through Cas3. After these initial steps, Cas3 can perform its helicase and nuclease functions simultaneously...likes to bluff, making you into think it’s more powerful than it is. The nuclease domain of Cas3 is considered...

  10. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...allows for a wide variety of screens, including interference, activation, and knockout. Most CRISPR screens...CRISPR Delivery Genome-wide Screening Using CRISPR Performing In Vivo CRISPR Screens Using the FITS Approach...

  11. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...every work setting and no manager will ever be perfect, but the chances of getting a good manager do tend...at once. Many companies will hire a postdoc to perform a specific project, while others will hire a group...

  12. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...student at the Max Planck Institute of Colloids and Interfaces. Here are some tips for cell culture that will...lab at the Max Planck Institute of Colloids and Interfaces. Sana is particularly interested in Glycobiology...

  13. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    ... NGA is a secondary PAM for SpCas9. Hu et al. performed GUIDE-seq analysis to characterize genome-wide...an NGG PAM, xCas9 3.7-VPR constructs modestly outperformed SpCas9-VPR in activating transcription. Sites...

  14. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...protein. The Bender’s popularity was short-lived as powerful molecular graphics packages were soon developed...channel.   He then said “I hadn’t realized how perfectly flat the complex is on this surface”….where the...

  15. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...have its limitations, especially when trying to perform high-throughput experiments. If a user wants to... modified to tag hundreds of genes, generating powerful genetic screening libraries. Additional protein...

  16. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    ...necessarily represent the views of Addgene. Addgene performs Sanger sequencing on select regions of all distributed...plasmids as part of quality control, but does not perform functional tests. The newest genome engineer sharing...

  17. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...from organism to organism. MMEJ doesn’t yield a “perfect repair” like HDR, but it’s much more predictable...ready-made PITCh plasmids available from Addgene are perfect for expressing GFP from a given promoter, and the...

  18. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    ...discuss how CRISPR libraries are being used to perform genome-wide screens and highlight some of the reagents...What are the steps involved in a CRISPR screen? Performing a forward genetic screen using CRISPR libraries...

  19. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...belongs to the penicillin family of antibiotics. It interferes with cell wall synthesis as its mode of action...Plasmids 101: Blue-White Screening Choosing Your Perfect Plasmid Backbone  ...

  20. Tips for arabidopsis transformation

    Type
    Blog Post
    ... when they begin flowering. It is important to perform the transformation not long after flowering begins...2007) ‘Improved Gateway Binary Vectors: High-Performance Vectors for Creation of Fusion Constructs in ...
Showing: 301 - 320 of 636 results