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We narrowed to 575 results for: multi

Showing: 301 - 320 of 575 results

  1. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    ...genome, they are not commonly used to introduce multiple mutations. This is because as more mutations are...challenge that may arise when crossing mice with multiple mutations is if the mutations are on the same ...

  2. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ...binding of the biosensor to endogenous calmodulin. Multiplexing Are you combining your calcium reading with ...you need to free up crowded optical space for multiplexing, GECO sensors are available in green, blue, ...

  3. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    ...pre-designed sets, so identical searches can return multiple answers. You might want to take advantage of their...SplitSet finds optimal fusion sites to split it into multiple fragments. You can define the number of fragments...

  4. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...elegant system that allows for the assembly of multiple DNA fragments efficiently and accurately in a ...system will allow investigators to rapidly create multiple synthetic chromatin-modifying proteins quickly...

  5. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...fast-growing cell lines (such as HEK293, which multiplies every 16 hours). So if you, for example, split... its passage number. Especially in a lab where multiple people are working on different things, this will...

  6. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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    Blog Post
    ...According to this logic, ω-dCas9 variants recognizing multiple PAMs should have a fitness advantage over proteins... and CAA PAMs, they saw that xCas9 3.7 cleaves multiple PAMs at a higher efficiency than SpCas9: NGT (...

  7. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ...editing efficiency of prime editing, whereby a multitude of configurations can be used to introduce specific...efficiencies for diverse prime editing systems in multiple cell types. Cell, 186, 1–17. https://doi.org/10.1016...

  8. Google Forums Round Up: First Impressions of NgAgo

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    Blog Post
    ..., the widespread success of CRISPR-Cas9 across multiple model organisms (3) lies in the versatility of...which could be less desirable if one has to target multiple sites in a single experiment. Also, the NGG PAM...

  10. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...attention to antibiotic selection. If you feel like multiple of the above antibiotics would work for you, they...Antibiotic Resistance Stability Time Saving?  Works for multiple organisms? Compatible with most bacteria? No satellite...

  11. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    ...  Skills such as research, collaboration across multiple disciplines, writing, project management, and ...knowledge of the 8 core competencies through a multiple choice exam.  The bottom line on being a grants...

  12. Plasmids 101: Protein tags

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    Blog Post
    ...depicted in the accompanying cartoon, they have a multitude of uses including (but not limited to) purification...localizes your protein to the nucleus? Or you want multiple rounds of purification to get your protein as ...

  13. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...cleavage system. LwaCas13a is also amenable to multiplexing; expressing 5 gRNAs simultaneously produces ... Featured Topic Page Learn about Anti-CRISPRs Multiplex Genome Editing with Cpf1 Resources on Addgene.org...

  14. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...interdisciplinarity index of research for single or multiple disciplines – you can select the disciplines and...networking, visual appeal, instant sharing across multiple other sites), I created a new platform for sharing...

  16. Bioinformatics at Addgene

    Type
    Blog Post
    ...inherent in Nextflow’s design, we were free to use multiple programming languages (Python, Perl, Java, R, ... adaptable property gave us the freedom to use multiple open-source sequence assembly tools at the appropriate...

  17. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ... the more complex Class I systems that contain multiple subunits, but the more they worked with it, the...high-fidelity rate. Both Cas7-11 and PASTE open up multitudes of possibilities at the bench and potentially...

  19. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    ...as those containing cassettes of the same gene multiple times.  Bioinformatics at Addgene After a run ...assembly. Geneious will show how reads align, reveal multiple contigs, and highlight sequence identities in ...
Showing: 301 - 320 of 575 results