We narrowed to 567 results for: multi
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TypeBlog Post...fast-growing cell lines (such as HEK293, which multiplies every 16 hours). So if you, for example, split... its passage number. Especially in a lab where multiple people are working on different things, this will...
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Google Forums Round Up: First Impressions of NgAgo
TypeBlog Post..., the widespread success of CRISPR-Cas9 across multiple model organisms (3) lies in the versatility of...which could be less desirable if one has to target multiple sites in a single experiment. Also, the NGG PAM... -
Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards
TypeBlog Post...then, using multiple confinement strategies is strongly advised. The effects are multiplicative, and there... -
Plasmids 101: Choosing an Antibiotic Resistance Gene
TypeBlog Post...attention to antibiotic selection. If you feel like multiple of the above antibiotics would work for you, they...Antibiotic Resistance Stability Time Saving? Works for multiple organisms? Compatible with most bacteria? No satellite... -
A Career in Grants: How To Become a Grant Professional
TypeBlog Post... Skills such as research, collaboration across multiple disciplines, writing, project management, and ...knowledge of the 8 core competencies through a multiple choice exam. The bottom line on being a grants... -
Plasmids 101: Protein tags
TypeBlog Post...depicted in the accompanying cartoon, they have a multitude of uses including (but not limited to) purification...localizes your protein to the nucleus? Or you want multiple rounds of purification to get your protein as ... -
Plasmids 101: Stringent Regulation of Replication
TypeBlog Post... must bind ATP. Then, the ATP-bound DnaA binds multiple specific sites within the ori called DnaA boxes... Multiple Authors... -
Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View
TypeBlog Post...researcher exquisite control over expression of multiple genes in specific cells at specific times. Many.... “A FLEX Switch Targets Channelrhodopsin-2 to Multiple Cell Types for Imaging and Long-Range Circuit ... -
CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?
TypeBlog Post...SunTag system which facilitates recruitment of multiple activation domains to the same genetic locus via... Multiple Authors... -
Bioinformatics at Addgene
TypeBlog Post...inherent in Nextflow’s design, we were free to use multiple programming languages (Python, Perl, Java, R, ... adaptable property gave us the freedom to use multiple open-source sequence assembly tools at the appropriate... -
New CRISPR Tools: Cas7-11 and PASTE
TypeBlog Post... the more complex Class I systems that contain multiple subunits, but the more they worked with it, the...high-fidelity rate. Both Cas7-11 and PASTE open up multitudes of possibilities at the bench and potentially... -
CRISPR 101: Targeting RNA with Cas13a (C2c2)
TypeBlog Post...cleavage system. LwaCas13a is also amenable to multiplexing; expressing 5 gRNAs simultaneously produces ... Featured Topic Page Learn about Anti-CRISPRs Multiplex Genome Editing with Cpf1 Resources on Addgene.org... -
Using Video to Share Your Science: We Share Science
TypeBlog Post...interdisciplinarity index of research for single or multiple disciplines – you can select the disciplines and...networking, visual appeal, instant sharing across multiple other sites), I created a new platform for sharing... -
Plasmids 101: E. coli Strains for Protein Expression
TypeBlog Post...reductase to faciliate proper folding; may increase multimer formation Δ(ara-leu)7697 ΔlacX74 ΔphoA PvuII ... Proteins which contain hydrophobic regions or multiple disulfide bonds may aggregate and become insoluble... -
Data Freedom: The Expansion of Data Sharing in Research Publications
TypeBlog Post...behavioral geneticists may track the motion of multiple animals on different occasions via video, generating...limiting numbers of figures such that many have multiple, complex panels. PLOS, itself, has not finalized... -
Hot Plasmids: Summer 2024
TypeBlog Post...be packaged in AAV vectors, potentially with multiplexed targets. Figure 4: CHARM uses a fusion... Multiple Authors... -
Antibodies 101: Beyond Surface Labeling
TypeBlog Post...These methods can include labeling markers with multiple proteins/antibodies, targeting markers found within...Otherwise, your secondary antibody will bind to multiple primary antibodies, yielding a false positive ... -
CRISPR 101: Homology Directed Repair
TypeBlog Post...Pfeiffer P, et al. Mutagenesis (2000). PubMed. Multiple Pathways of Recombination Induced by Double-Strand... Multiple Authors... -
CRISPR 101: Validating Your Genome Edit
TypeBlog Post... words, any insertion or deletion that isn’t a multiple of three will do. A popular screening method for... Multiple Authors... -
Filming Science Videos in the Age of Social Distancing
TypeBlog Post... scrambling. Everything we had set up involved multiple people in tight locations. Conference rooms for...to the talent’s shirt. But knowing I would have multiple interview subjects, I positioned a boom mic just...