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Showing: 301 - 320 of 567 results
  1. 10 Basic tips for mammalian cell culture

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    Blog Post
    ...fast-growing cell lines (such as HEK293, which multiplies every 16 hours). So if you, for example, split... its passage number. Especially in a lab where multiple people are working on different things, this will...
  2. Google Forums Round Up: First Impressions of NgAgo

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    Blog Post
    ..., the widespread success of CRISPR-Cas9 across multiple model organisms (3) lies in the versatility of...which could be less desirable if one has to target multiple sites in a single experiment. Also, the NGG PAM...
  3. Plasmids 101: Choosing an Antibiotic Resistance Gene

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    Blog Post
    ...attention to antibiotic selection. If you feel like multiple of the above antibiotics would work for you, they...Antibiotic Resistance Stability Time Saving?  Works for multiple organisms? Compatible with most bacteria? No satellite...
  4. A Career in Grants: How To Become a Grant Professional

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    Blog Post
    ...  Skills such as research, collaboration across multiple disciplines, writing, project management, and ...knowledge of the 8 core competencies through a multiple choice exam.  The bottom line on being a grants...
  5. Plasmids 101: Protein tags

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    Blog Post
    ...depicted in the accompanying cartoon, they have a multitude of uses including (but not limited to) purification...localizes your protein to the nucleus? Or you want multiple rounds of purification to get your protein as ...
  6. Bioinformatics at Addgene

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    Blog Post
    ...inherent in Nextflow’s design, we were free to use multiple programming languages (Python, Perl, Java, R, ... adaptable property gave us the freedom to use multiple open-source sequence assembly tools at the appropriate...
  7. New CRISPR Tools: Cas7-11 and PASTE

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    Blog Post
    ... the more complex Class I systems that contain multiple subunits, but the more they worked with it, the...high-fidelity rate. Both Cas7-11 and PASTE open up multitudes of possibilities at the bench and potentially...
  8. CRISPR 101: Targeting RNA with Cas13a (C2c2)

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    Blog Post
    ...cleavage system. LwaCas13a is also amenable to multiplexing; expressing 5 gRNAs simultaneously produces ... Featured Topic Page Learn about Anti-CRISPRs Multiplex Genome Editing with Cpf1 Resources on Addgene.org...
  9. Using Video to Share Your Science: We Share Science

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    Blog Post
    ...interdisciplinarity index of research for single or multiple disciplines – you can select the disciplines and...networking, visual appeal, instant sharing across multiple other sites), I created a new platform for sharing...
  10. Plasmids 101: E. coli Strains for Protein Expression

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    Blog Post
    ...reductase to faciliate proper folding; may increase multimer formation Δ(ara-leu)7697 ΔlacX74 ΔphoA PvuII ... Proteins which contain hydrophobic regions or multiple disulfide bonds may aggregate and become insoluble...
  11. Hot Plasmids: Summer 2024

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    Blog Post
    ...be packaged in AAV vectors, potentially with multiplexed targets.   Figure 4: CHARM uses a fusion...  Multiple Authors...
  12. Antibodies 101: Beyond Surface Labeling

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    Blog Post
    ...These methods can include labeling markers with multiple proteins/antibodies, targeting markers found within...Otherwise, your secondary antibody will bind to multiple primary antibodies, yielding a false positive ...
  13. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...Pfeiffer P, et al. Mutagenesis (2000). PubMed. Multiple Pathways of Recombination Induced by Double-Strand... Multiple Authors...
  14. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ... scrambling. Everything we had set up involved multiple people in tight locations. Conference rooms for...to the talent’s shirt. But knowing I would have multiple interview subjects, I positioned a boom mic just...
Showing: 301 - 320 of 567 results