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Showing: 301 - 320 of 381 results

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Mouse Modeling, Part 1: Genetically Engineered Mice

Type

Blog Post

...Outbred strains of mice are useful for comparing dominant and recessive traits and their impact on diseases...
Viral Vectors 101: Chemogenetics

Type

Blog Post

...Snowball A, Knauss S, von Schimmelmann M, During MJ, Lignani G, Schorge S, Young D, Kullmann DM, Lieb A (2019...
Plasmids 101: FLEx Vectors

Type

Blog Post

...Saunders A, Johnson CA, Sabatini BL. 2012. Novel recombinant adeno-associated viruses for Cre activated and...
Plasmids 101: Terminators and PolyA signals

Type

Blog Post

...many studies focus on promoter strength as a determinant of gene expression levels, the terminator also...
Experimenting in an Open Source lab: from CRISPR to Cats

Type

Blog Post

... a new project at a makerspace is finding the financial resources to pursue it. Since makerspaces are ...
Plasmids 101: 5 factors to help you choose the right cloning method

Type

Blog Post

... to the use of toxic ccdB to prevent any non-recombinant vectors from propagating. The ligation-independent...
Fluorescent Tagging of Endogenous Genes with SapTrap

Type

Blog Post

...plasmids for protein tagging, including luciferase (NanoLuc), fluorescent proteins (TagGFP2, TagBFP, TagRFP...
Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

Type

Blog Post

...feature of balancer chromosomes is that they produce dominant phenotypes visible on the flies. For example, ...
Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

Type

Blog Post

...between two molecules more closely is Förster Resonance Energy Transfer, FRET. Additional opportunities...
Truncated gRNAs for Regulating Gene Expression

Type

Blog Post

...where her research is focused on engineering nanoparticles as drug delivery systems. She is also a NASA...
Plasmids 101: Knockout/Knock-In Plasmids

Type

Blog Post

...marker HSV-tk is used to select against random recombinants. Design your targeting construct. For...
Production of Virus in Insect Versus Mammalian Cells

Type

Blog Post

...). HEK293 cell line as a platform to produce recombinant proteins and viral vectors. Frontiers in Bioengineering...
Pooled Library Amplifications

Type

Blog Post

...For larger libraries that require hundreds of nanograms of DNA, you will likely need to perform several...
Chromoproteins: Colorful Proteins For Molecular Biology Experiments

Type

Blog Post

...fluorescence lifetime imaging microscopy-based Förster resonance energy transfer (FLIM-FRET) in living cells (Figure...
Antibodies 101: Conventional vs Spectral Flow Cytometry

Type

Blog Post

...Antibody GuideAddgene's collection of ready-to-use recombinant antibodies...
When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

Type

Blog Post

...particular type will interact. A protein with a nanomolar Kd will exist primarily as a dimer in cells while...
PITChing MMEJ as an Alternative Route for Gene Editing

Type

Blog Post

...which has been difficult to modify due to NHEJ’s dominance over HDR in this species. As CRISPR technology...
Which Fluorescent Protein Should I Use?

Type

Blog Post

...effect called FRET (fluorescence [or Förster] resonance energy transfer). FRET occurs when energy transfer...
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

Type

Blog Post

...Medical Center. You’ve probably heard of Forster Resonance Energy Transfer (FRET). Through the non-radiative...
Droplet Digital PCR for AAV Quantitation

Type

Blog Post

...tool for titration and integrity evaluation of recombinant adeno-associated viral vectors." Human Gene Therapy...

Showing: 301 - 320 of 381 results

Results per page:

Mouse Modeling, Part 1: Genetically Engineered Mice

Viral Vectors 101: Chemogenetics

Plasmids 101: FLEx Vectors

Plasmids 101: Terminators and PolyA signals

Experimenting in an Open Source lab: from CRISPR to Cats

Plasmids 101: 5 factors to help you choose the right cloning method

Fluorescent Tagging of Endogenous Genes with SapTrap

Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

Truncated gRNAs for Regulating Gene Expression

Plasmids 101: Knockout/Knock-In Plasmids

Production of Virus in Insect Versus Mammalian Cells

Pooled Library Amplifications

Chromoproteins: Colorful Proteins For Molecular Biology Experiments

Antibodies 101: Conventional vs Spectral Flow Cytometry

When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

PITChing MMEJ as an Alternative Route for Gene Editing

Which Fluorescent Protein Should I Use?

Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

Droplet Digital PCR for AAV Quantitation

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