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We narrowed to 449 results for: organization Addgene

Showing: 301 - 320 of 449 results

  1. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type
    Blog Post
    ... specificity of Cas9s isolated from different organisms and engineered Cas9 variants that were able to...PMCID: PMC6255809.  Additional resources on the Addgene blog Read our CRISPR 101 blog posts Find more ... Download the CRISPR 101 eBook Resources on Addgene.org Visit our CRISPR plasmids and resources page...

  2. Viral Vectors 101: Biosensors

    Type
    Blog Post
    ...analyte. This can be difficult to achieve in whole organisms, but AAVs make it possible. In AAV systems, different...AAV Viral Vectors 101 eBook Resources on the Addgene blog An Overview of AAV Encoded Calcium Biosensors.... DOI: 10.3389/fncel.2019.00474 Resources on Addgene.org Fluorescent Proteins: Biosensors Viral Service...

  3. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ...disciplinary boundaries and can allow for innovative organizations and companies to provide problem-solving platforms...shows workflow of open hardware.    Conclusion Addgene is a great example of how sharing of data and molecular...

  4. All in a Twist: dsRNA

    Type
    Blog Post
    ...previous chemical versions with toxicity to many organisms. Fig. 1: Exogenous sources of dsRNA ....1038/s41580-021-00430-1 More resources on the Addgene blog CRISPR 101: RNA Editing with Cas13  RNA...cellular dysregulation) is the mitochondria. These organelles have their own circular genomes which can produce...

  5. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type
    Blog Post
    ...embryos and adult fish. Zebrafish are an ideal model organism for these experiments because developing embryos...PubMed PMID: 16369538. Additional Resources on the Addgene Blog Read Other Optogenetics Blog Posts Sign Up...about Small Molecule Fluorophores Resources on Addgene.org Visit Our Fluorescent Protein Guide Page Check...

  6. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    ...careers through structural change in research organizations. “Fix the Knowledge” or “gendered innovations...Technology” in 2011 and again in 2014. Resources on the Addgene Blog Learn How Scientists Are Trying to Shape ...

  7. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type
    Blog Post
    ...Guide to Viral Vectors Resources on Addgene.org Learn about Addgene's viral service Find viral protocols...choice for many behavioral studies, though other organisms such as rats or zebrafish are also used. With ...detail and share ways how scientists have used Addgene’s plasmid repository and viral service for their...PMCID: PMC4135440.  Additional resources on the Addgene blog Read all of our viral vectors blog posts ...

  8. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ... of your organization Time management Managing a team Leadership Resources on Addgene.org Check out...training in a variety of transferable skills. At Addgene, we've set out to fill this gap by both highlighting...currently work on the Scientific Support Team here at Addgene, so troubleshooting molecular biology experiments...choose.    Resources Additional resources on the Addgene blog More from the transferable skills guide: ...

  10. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ...illuminate cell organization." bioRxiv (2017): 123042. Additional Resources on the Addgene Blog Learn More...with GFP. These plasmids are available through Addgene, and the stem cell lines we’ve made using them ...a variety of endogenous gene tagging systems. Addgene’s CRISPR-based Protein Tagging collection includes... Our Fluorescent Protein Posts Resources on Addgene.org Browse CRISPR Tagging Plasmids Browse the Fluorescent...

  11. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    ...career offices, postdoc offices or professional organizations can provide referrals. There are bad coaches...suggest some coaches? Yes I can. Please note that Addgene does not endorse any of these coaches and I can't...investment in your future. Additional Resources on the Addgene Blog 6 Steps to Submitting a Resume That Gets ...Future of Research Symposium Resources on the Addgene Website Check out Our Career Page for Recent Job...

  12. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    ...optogenetics Resources on Addgene.org Browse all viral vectors available at Addgene Visit our viral vectors...inside a mouse’s brain.  But the brain is a tricky organ to access, which often forces scientists to use .../s41592-019-0583-8 Additional resources on the Addgene blog Read more about the PHP.eB AAV capsid Read...

  13. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...alternative option but it’s toxic to tissues and model organisms. Detecting RNA-protein interactions with RaPID... answer? The RaPID plasmids are available from Addgene! References Ramanathan, M., Majzoub, K., Rao, ...PMCID: PMC4054858.   Additional Resources on the Addgene Blog Study Protein-DNA Interactions with CUT&RUN...resources for studying chromatin Resources on Addgene.org Find Split-BioID plasmids Find CRISPR plasmids...

  14. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    ...guest blogger and Future of Research Symposium organizer, David T. Riglar PhD. Here, Dr. Riglar discusses...biomedical_research_wgreport.pdf Resources at Addgene Learn More about Careers in Science in Our Career...Advice on Networking at Scientific Meetings from Addgene Executive Director Joanne Kamens Learn how to be...experience from the perspective of both grassroots organisations that represent postdocs and institutes that ...

  15. Tips for Technical Support Calls

    Type
    Blog Post
    ...Resources on the Addgene Blog Learn About the Addgene Help Center Get Help with the Addgene Deposit Process...emails in my six years as a senior scientist at Addgene. Having spent that long in customer service, I'...Though my experience is based solely on my time at Addgene, I’m confident that these tips and tricks will ... the company’s FAQs? A lot of companies and organizations selling biological reagents have a ton of data...another great source of information. At least at Addgene, the questions in our FAQ section are really, truly...not realize it, all of the reagents available at Addgene come from researchers, and the vast majority of...happens more frequently than you might think. The Addgene scientists answering the phone are smart, but they...

  16. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...addition to useful plasmids and toolkits available at Addgene.  C. elegans open resources The C. elegans Neuronal...source project that is creating the “first virtual organism in a computer.” This project has produced various...Kyoda et al., 2012).  C. elegans plasmid tools at Addgene In addition to the wealth of open resources described...highlight a few toolkits that we have available at Addgene that were discussed primarily at the workshops ...interest.  SapTrap Tool Kit for CRISPR Cas9 edits At Addgene, we have the entire SapTrap toolkit from the Jorgensen...resources about C. elegans and the plasmids we have at Addgene check out our worm expression resources page. This...PMCID: PMC4689222. Additional resources on the Addgene blog Learn more about SapTrap Read about CRISPR...

  17. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ...maps. Resources on Addgene.org Sequence Analysis of your Addgene Plasmid Addgene Plasmid Guide     ...This post was updated on Dec 4, 2017. At Addgene, we continually use the Basic Local Alignment Search...databases, and elements of the BLAST search pages. At Addgene, we use blastn to identify any discrepancies in..., enter the common or scientific name into the Organism box. This small piece of information can significantly... viewing blastx results. When using blastx at Addgene, we use the default “Non-redundant protein sequences...NCBI BLAST website. Additional Resources on the Addgene Blog: Plasmids 101: How to Verify Your Plasmid...

  18. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...Guillain-Barré syndrome. In response, the World Health Organization has declared a global health emergency and called...corresponding portions of the Zika virus genome with Addgene to facilitate rapid sharing of these tools with... community. Find the Zika Sensor Constructs at Addgene As we report, we have embedded this next generation...27160350. Find Plasmids from the publication at Addgene 2. Green, Alexander A., et al. "Toehold switches...e15272. PubMed PMID: 27090089. Resources on the Addgene Blog Read other Synthetic Biology Posts Listen...Your Next Experiment Deposit Your Plasmids with Addgene ...Used to Study Dangerous Viruses Resources on Addgene.org Check out All the Plasmids from the Collin's...

  19. Genetic Code Expansion

    Type
    Collection
    ...Addgene's guide to plasmids and strains useful for expanding the genetic code in bacteria and mammalian...pairs of synthetase and tRNA that work for one organism may not work for another. The orthogonal synthetase...

  20. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...Alternatively, gRNA-only plasmids can be used in cells or organisms in which Cas9 has been integrated into the genome...Many gRNA empty vectors have been deposited at Addgene. To find the gRNA vector for your system, you can...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...
Showing: 301 - 320 of 449 results