...Alternatively, gRNA-only plasmids can be used in cells or organisms in which Cas9 has been integrated into the genome...Many gRNA empty vectors have been deposited at Addgene. To find the gRNA vector for your system, you can...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

... a repository others could request from — Addgene!  Addgene was established as an e-commerce company, ...a resource like Addgene to all get the same virus.” — Grad student visiting Addgene’s booth at the Society...information across the globe.  “Read the Addgene guide: https://www.addgene.org/guides/crispr/” — SirHamhands, ...young again! Addgenie Marcy (now our Sr. Director of Production) pie-fully celebrating Addgene's 10 year anniversary...Addgene is ending 2024 with big plans for our future. And while we’ve been excited to share them with...thoughts from the community that makes us… us. “The Addgene not-for-profit is an amazing service to biology...Lunenfeld-Tanenbaum Research Institute, posting about Addgene’s 20th anniversary on Twitter. In the beginning ...

...in Molecular Biology. This annual symposium is organized by international PhD students at the Max Planck...PMCID: PMC4121734. Additional Resources on the Addgene Blog Fluorescent microscopy techniques - Which...protein-protein interactions Deposit plasmids with Addgene! ... tagging strategies Additional Resources on Addgene.org Fluorescent protein plasmids Use FRET to study...

...Sequencing the 16S rRNA reveals the identity of the organisms present while sequencing of all transcripts gives...PubMed Central PMCID: PMC6104080. Resources on the Addgene Blog Find microbiology blog posts Read about synthetic...Deposit microbiology plasmids with Addgene Find synthetic biology plasmids ...of our Plasmids 101 blog posts Resources on Addgene.org Check out our microbiology resource page Deposit...

...plasmids from this publication at Addgene. Additional Resources on the Addgene Blog Read More about Fluorescent...helping researchers visualize the genome and its organization within the nucleus, also called the 4D nucleome...cells! This technique was developed in the lab of Addgene depositor Stanley Qi and used in Chen et al. to...colorful? CRISPRainbow is available as a kit from Addgene.   References 1. Chen, Baohui, et al. “Dynamic...PMC3918502. Find plasmids from this publication at Addgene. 2. Ma, Hanhui, et al. “Multicolor CRISPR labeling...PMC4364232. Find the plasmids from this publication at Addgene. 3. Ma, Hanhui, et al. “Multiplexed labeling of...PMC4864854. Find the plasmids from this publication at Addgene. 4. Kiani, Samira, et al. “Cas9 gRNA engineering...

...embryos and adult fish. Zebrafish are an ideal model organism for these experiments because developing embryos...PubMed PMID: 16369538. Additional Resources on the Addgene Blog Read Other Optogenetics Blog Posts Sign Up...about Small Molecule Fluorophores Resources on Addgene.org Visit Our Fluorescent Protein Guide Page Check...

...disciplinary boundaries and can allow for innovative organizations and companies to provide problem-solving platforms...shows workflow of open hardware.    Conclusion Addgene is a great example of how sharing of data and molecular...

...previous chemical versions with toxicity to many organisms. Fig. 1: Exogenous sources of dsRNA ....1038/s41580-021-00430-1 More resources on the Addgene blog CRISPR 101: RNA Editing with Cas13  RNA...cellular dysregulation) is the mitochondria. These organelles have their own circular genomes which can produce...

...analyte. This can be difficult to achieve in whole organisms, but AAVs make it possible. In AAV systems, different...AAV Viral Vectors 101 eBook Resources on the Addgene blog An Overview of AAV Encoded Calcium Biosensors.... DOI: 10.3389/fncel.2019.00474 Resources on Addgene.org Fluorescent Proteins: Biosensors Viral Service...

... specificity of Cas9s isolated from different organisms and engineered Cas9 variants that were able to...PMCID: PMC6255809.  Additional resources on the Addgene blog Read our CRISPR 101 blog posts Find more ... Download the CRISPR 101 eBook Resources on Addgene.org Visit our CRISPR plasmids and resources page...

...careers through structural change in research organizations. “Fix the Knowledge” or “gendered innovations...Technology” in 2011 and again in 2014. Resources on the Addgene Blog Learn How Scientists Are Trying to Shape ...

...Additional Resources on the Addgene Blog Volunteer to write for the Addgene blog Listen to Our Podcast...Communication Posts Resources on Addgene.org Explore careers at Addgene Other Resources The National ...government. All the Health and Human Services (HHS) organizations have so many [science communication] needs. ...

... of your organization Time management Managing a team Leadership Resources on Addgene.org Check out...training in a variety of transferable skills. At Addgene, we've set out to fill this gap by both highlighting...currently work on the Scientific Support Team here at Addgene, so troubleshooting molecular biology experiments...choose.    Resources Additional resources on the Addgene blog More from the transferable skills guide: ...

...Guide to Viral Vectors Resources on Addgene.org Learn about Addgene's viral service Find viral protocols...choice for many behavioral studies, though other organisms such as rats or zebrafish are also used. With ...detail and share ways how scientists have used Addgene’s plasmid repository and viral service for their...PMCID: PMC4135440.  Additional resources on the Addgene blog Read all of our viral vectors blog posts ...

...illuminate cell organization." bioRxiv (2017): 123042. Additional Resources on the Addgene Blog Learn More...with GFP. These plasmids are available through Addgene, and the stem cell lines we’ve made using them ...a variety of endogenous gene tagging systems. Addgene’s CRISPR-based Protein Tagging collection includes... Our Fluorescent Protein Posts Resources on Addgene.org Browse CRISPR Tagging Plasmids Browse the Fluorescent...

...career offices, postdoc offices or professional organizations can provide referrals. There are bad coaches...suggest some coaches? Yes I can. Please note that Addgene does not endorse any of these coaches and I can't...investment in your future. Additional Resources on the Addgene Blog 6 Steps to Submitting a Resume That Gets ...Future of Research Symposium Resources on the Addgene Website Check out Our Career Page for Recent Job...

...Resources on the Addgene Blog Learn About the Addgene Help Center Get Help with the Addgene Deposit Process...emails in my six years as a senior scientist at Addgene. Having spent that long in customer service, I'...Though my experience is based solely on my time at Addgene, I’m confident that these tips and tricks will ... the company’s FAQs? A lot of companies and organizations selling biological reagents have a ton of data...another great source of information. At least at Addgene, the questions in our FAQ section are really, truly...not realize it, all of the reagents available at Addgene come from researchers, and the vast majority of...happens more frequently than you might think. The Addgene scientists answering the phone are smart, but they...

...alternative option but it’s toxic to tissues and model organisms. Detecting RNA-protein interactions with RaPID... answer? The RaPID plasmids are available from Addgene! References Ramanathan, M., Majzoub, K., Rao, ...PMCID: PMC4054858.   Additional Resources on the Addgene Blog Study Protein-DNA Interactions with CUT&RUN...resources for studying chromatin Resources on Addgene.org Find Split-BioID plasmids Find CRISPR plasmids...

...optogenetics Resources on Addgene.org Browse all viral vectors available at Addgene Visit our viral vectors...inside a mouse’s brain.  But the brain is a tricky organ to access, which often forces scientists to use .../s41592-019-0583-8 Additional resources on the Addgene blog Read more about the PHP.eB AAV capsid Read...

...guest blogger and Future of Research Symposium organizer, David T. Riglar PhD. Here, Dr. Riglar discusses...biomedical_research_wgreport.pdf Resources at Addgene Learn More about Careers in Science in Our Career...Advice on Networking at Scientific Meetings from Addgene Executive Director Joanne Kamens Learn how to be...experience from the perspective of both grassroots organisations that represent postdocs and institutes that ...