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We narrowed to 466 results for: organization Addgene

Showing: 301 - 320 of 466 results

  1. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ...NBK19423/ Resources on Addgene.org Addgene's most popular lentiviral plasmids Addgene's most popular retroviral...retroviral plasmids Addgene's collection of AAV plasmids Addgene’s collection of adenovirus plasmids Resources...of viral vectors here! Need Virus? Check out Addgene's Viral Service! Types of viral vectors Of the many..., typically an expression construct, to cells/organisms. This is often cDNA, shRNA, or something similar...components of adenovirus Building viral vectors Addgene has many viral vectors and helper plasmids for ...Resources on the Addgene blog Parts of the AAV packaging plasmid Overview of Lentiviral Packaging Plasmids...

  2. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    ... the effects of the drug on the actual cells or organ system. This allows scientists to get a more definitive...and current Scientist, DNA Sequencing and QC at Addgene.  References and resources References Mosch, ...

  3. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    ...Bench setup Small adjustments in the physical organization of your workspace and your workflow can have...documents/Western%20Blotting.pdf  More resources on Addgene’s blog A Guide to Getting Started in Undergrad ...

  4. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

    Type
    Blog Post
    ...) is the movement of genetic material between organisms. It plays a key role in bacterial evolution and...and thus transformation efficiency. Check out Addgene’s blog to learn about making your own competent ... as well as ready-to-use viral preparations at Addgene. For more information on viral vectors, including...starting any molecular biology experiment check out Addgene’s protocol page, which has both protocols and videos...PubMed PMID: 16357262. Additional resources on the Addgene blog Read all of our Plasmids 101 blog posts Check... blog posts on plasmid cloning Resources on Addgene.org Read our protocols for plasmid cloning Find ...

  5. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type
    Blog Post
    ...several days. In 2015, the laboratory of long-time Addgene depositor Alice Ting used yeast display-based directed...makes PL a viable method for proteomics in model organisms in which it was previously not an option, including...toehold switches to detect Zika virus (available at Addgene). The plasmids for detecting norovirus will be ...SH3 domain containing plasmids available through Addgene. These plasmids can be used to carry out any biochemical...hundreds of additional plasmids available through Addgene, for study of the of WW, PDZ, and other protein...A fundamental approach to studying membranes, organelles, and other subcellular compartments is via proteomics... Various Addgenies...

  6. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    ...resources on the Addgene blog: Addgene Optogenetics guide Primer on Optogenetics blog series Addgene Biosensors...patterning activities of entire tissue types or even organs. In frogs, ectopic expression of ion channels, ...discussed in Mathews and Levin, 2018) available at Addgene: MerMAID2 - A metagenomically discovered marine...

  7. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ...resources Addgene’s viral services: AAV Resources on the Addgene blog Viral Vectors 101: Viral Vector...v13102073 Resources on Addgene.org Adeno-associated Virus (AAV) Guide Addgene’s viral plasmids and resources...interest. This is a particularly nice feature as organ-specific injections, or the location of a specific...

  8. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ... your CRISPR experiment was successful in your organism or cell type? You can use DNA sequencing or other...desired genomic edit.  Find SRIRACCHA plasmids at Addgene GEMCherry2, an improved monomeric fluorescent protein...stem cells.  Find GEmCherry2 reporter plasmids at Addgene Overall SRIRACHA and GEmCherry2 reporter systems.../jbc.m117.777722 Additional resources on the Addgene blog Browse all of our CRISPR blog posts Find ...with our CRISPR 101 blog posts Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids for...

  9. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    ...resources on the Addgene blog Find our synthetic biology blog posts Resources on Addgene.org Find plasmids...basics.  Mesophiles vs. extremophiles Mesophilic organisms are your regular next-door microbes which grow... pC013-Twinstrep-SUMO-huLwCas13a plasmids from Addgene, which would potentially allow a stable Cas expression...” by Benoit Giquel, an outreach scientist from Addgene. The talk highlighted the importance of reagent...

  10. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    ...Paolo Colombi, a product development scientist at Addgene. Cloning can be quite an arduous process. The PCR...want to amplify sequences from a large genome organisms like mouse or human. There are several online ...currently the product development scientist at Addgene. In the lab he develops new assays and processes...possible aspects.     Additional Resources on the Addgene Blog Browse our Plasmids 101 blog posts Read blog...about different plasmid elements Resources on Addgene.org Find protocols for plasmid cloning Read our ...

  11. Addgene: The First Twenty Years

    Type
    Blog Post
    ... a repository others could request from — Addgene!  Addgene was established as an e-commerce company, ...a resource like Addgene to all get the same virus.” — Grad student visiting Addgene’s booth at the Society...information across the globe.  “Read the Addgene guide: https://www.addgene.org/guides/crispr/” — SirHamhands, ...young again! Addgenie Marcy (now our Sr. Director of Production) pie-fully celebrating Addgene's 10 year anniversary...Addgene is ending 2024 with big plans for our future. And while we’ve been excited to share them with...thoughts from the community that makes us… us. “The Addgene not-for-profit is an amazing service to biology...Lunenfeld-Tanenbaum Research Institute, posting about Addgene’s 20th anniversary on Twitter. In the beginning ...

  12. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ...in Molecular Biology. This annual symposium is organized by international PhD students at the Max Planck...PMCID: PMC4121734. Additional Resources on the Addgene Blog Fluorescent microscopy techniques - Which...protein-protein interactions Deposit plasmids with Addgene! ... tagging strategies Additional Resources on Addgene.org Fluorescent protein plasmids Use FRET to study...

  13. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...Sequencing the 16S rRNA reveals the identity of the organisms present while sequencing of all transcripts gives...PubMed Central PMCID: PMC6104080. Resources on the Addgene Blog Find microbiology blog posts Read about synthetic...Deposit microbiology plasmids with Addgene Find synthetic biology plasmids ...of our Plasmids 101 blog posts Resources on Addgene.org Check out our microbiology resource page Deposit...

  14. Plasmids for Stem Cell Research

    Type
    Collection
    ...Browse Addgene's collection of plasmids for the generation and study of stem cells, from iPSC generation...and have the capacity to give rise to an entire organism. iPSC technology removes the possible ethical ...may be difficult to decide which method to use. Addgene's blog post Delivery Methods for Generating iPSCs...know which method you would like to use, browse Addgene's collection of Reprogramming Plasmids in the table...cells or fully differentiated cell types. Browse Addgene's collection of Differentiation Plasmids below. ...inherent disadvantage of iPS cell technology. Browse Addgene's collection of Transdifferentiation Plasmids below...below will help you find plasmids available from Addgene that can be used to create iPSCs or another cell...

  15. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...plasmids from this publication at Addgene. Additional Resources on the Addgene Blog Read More about Fluorescent...helping researchers visualize the genome and its organization within the nucleus, also called the 4D nucleome...cells! This technique was developed in the lab of Addgene depositor Stanley Qi and used in Chen et al. to...colorful? CRISPRainbow is available as a kit from Addgene.   References 1. Chen, Baohui, et al. “Dynamic...PMC3918502. Find plasmids from this publication at Addgene. 2. Ma, Hanhui, et al. “Multicolor CRISPR labeling...PMC4364232. Find the plasmids from this publication at Addgene. 3. Ma, Hanhui, et al. “Multiplexed labeling of...PMC4864854. Find the plasmids from this publication at Addgene. 4. Kiani, Samira, et al. “Cas9 gRNA engineering...

  16. Viral Vectors 101: Biosensors

    Type
    Blog Post
    ...analyte. This can be difficult to achieve in whole organisms, but AAVs make it possible. In AAV systems, different...AAV Viral Vectors 101 eBook Resources on the Addgene blog An Overview of AAV Encoded Calcium Biosensors.... DOI: 10.3389/fncel.2019.00474 Resources on Addgene.org Fluorescent Proteins: Biosensors Viral Service...

  17. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ...disciplinary boundaries and can allow for innovative organizations and companies to provide problem-solving platforms...shows workflow of open hardware.    Conclusion Addgene is a great example of how sharing of data and molecular...

  18. All in a Twist: dsRNA

    Type
    Blog Post
    ...previous chemical versions with toxicity to many organisms. Fig. 1: Exogenous sources of dsRNA ....1038/s41580-021-00430-1 More resources on the Addgene blog CRISPR 101: RNA Editing with Cas13  RNA...cellular dysregulation) is the mitochondria. These organelles have their own circular genomes which can produce...

  19. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type
    Blog Post
    ... specificity of Cas9s isolated from different organisms and engineered Cas9 variants that were able to...PMCID: PMC6255809.  Additional resources on the Addgene blog Read our CRISPR 101 blog posts Find more ... Download the CRISPR 101 eBook Resources on Addgene.org Visit our CRISPR plasmids and resources page...

  20. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type
    Blog Post
    ...embryos and adult fish. Zebrafish are an ideal model organism for these experiments because developing embryos...PubMed PMID: 16369538. Additional Resources on the Addgene Blog Read Other Optogenetics Blog Posts Sign Up...about Small Molecule Fluorophores Resources on Addgene.org Visit Our Fluorescent Protein Guide Page Check...
Showing: 301 - 320 of 466 results