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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 563 results for: pac

Showing: 301 - 320 of 563 results

  1. Engaging with science and society at pgEd

    Type
    Blog Post
    ...But as the science moves forward at a blistering pace, it is becoming ever more urgent for scientists ...at pgEd, I take on a variety of projects in the space of science education, communication, and policy....

  3. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    ...breed. Some problems with transgenic mice that can impact reproduction include embryonic lethality, a short...should be tested in cell lines to determine how it impacts the expression level of the transgene. In existing...

  4. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ...and selection markers. Need fewer components? A spacer can be inserted instead, allowing everything to...entirely, but instead will have to replace it with a spacer.) Each component available on the Fragmid website...

  5. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ...FRET pair. If you need to free up crowded optical space for multiplexing, GECO sensors are available in ...the calcium sensors described are also already packaged into viral vectors and ready to go. Pro tip: Vectors...

  6. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...allows you to study signaling pathways and the impacts of neural circuits on behavior. Figure 1:...channels. When working with PSAM receptors, your impact on the cell is determined by what IPD your PSAM...

  7. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...Gag structural protein on yet a third plasmid (packaging plasmid). Viral production then proceeds by transfection....   Figure 1: Separation of retroviral packaging components onto distinct plasmids for safety.  ...

  8. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...depiction of chromatin within a nucleus -> DNA compaction around histones -> Examples of histone-modifying...flanked by randomized nucleotides in place of a protospacer adjacent motif (PAM). They're used to determine...

  9. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ...counterparts, so they will be easier to deliver in low-capacity vectors like AAV. AAV (adeno-associated viral ...tissues. However, these vectors have a low genetic capacity of ~4.5 kb. At 4.1 kb, SpCas9 barely fits into...

  11. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...that is complementary to the target site, or protospacer, in the genome. Native Cas9 has two catalytic...which cleaves one strand of DNA upon binding the protospacer. The resulting double strand break (DSB) stimulates...

  12. Common Injection Routes in Mice

    Type
    Blog Post
    ...injections are given in the peritoneal cavity, or the space surrounding organs in the abdomen. They are a popular...cerebral spinal fluid. The injection site is the space in and around the vertebrae, so hitting the correct...

  13. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...genomic integration. Lab-engineered viruses will package your genetic cargo in lieu of their own genomes...genomes and deposit this cargo upon host infection. Packaging viruses also have various serotypes – the surface...

  15. PhD Applications After COVID

    Type
    Blog Post
    ...with picking universities?” and “How does COVID-19 impact all of this?” are all very valid questions, and...program will allow for you to have the time and space to delve into what matters to you. It can also be...

  16. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...mutations which do not interfere with ACE2 binding, but impact recognition by specific anti-SARS-CoV-2 antibodies...intron-optimized SpCas9-coding gene. SlugCas9 is a compact Cas9 ortholog from Staphylococcus lugdunensis that...

  17. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...a team to develop therapies that could directly impact patients. And, to be honest, I’ve never enjoyed...better understanding of the fast, deadline-driven pace of biotech. In other words, industry postdocs already...

  18. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ... of the protein’s backbone in three-dimensional space, but their flexible and jiggly nature suggested ...was short-lived as powerful molecular graphics packages were soon developed that allowed researchers to...

  20. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...integration into the genome, and its large DNA packaging capacity is well suited for larger transgenes. Unfortunately...
Showing: 301 - 320 of 563 results