We narrowed to 407 results for: pho

1. Visualizing Protein Turnover In Situ

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   Blog Post

   ...Which is best for me? Learn how small molecule fluorophores can be used for cell labeling Check out other...

2. Summer SciComm: Preprints

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   Blog Post

   ... CERN — it's a new age of accelerating science! Photo courtesy of Henry Mühlpfordt, under a CC BY-SA license...

3. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

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   ...al disrupted interactions between Cas9 and the phophate backbone of the target DNA strand via mutations...

4. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

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   ...FPs optimized for oxidizing environments to photoconvertible variants, it seems like GFP is always learning...

5. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

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   ...Louisiana State University. She is a fan of photosynthesis and writes about autotrophs and biochemistry...

6. Hot Plasmids February 2024

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   ...Ando et al., 2023). This variant has similar photostability and brightness compared to the original, though...

7. CRISPR in the Clinic

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   Blog Post

   ...CRISPR spin. CRISPR CAR-T treatments for leukemia, lymphoma, and some solid tumors are currently in early-...

8. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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   ...quantification of various intermediate steps, a sizing electrophoretic gel, and qPCR quantification of the final libraries...

9. Hot Plasmids and Viral Preps - March 2021

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   ....   G-baToN can also be applied to other fluorophore/antibody pairs. Because the anti-GFP nanobody...

10. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

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    Blog Post

    ...CRISPR-Cas activity. For example, fusing AcrA4II to the photo-sensitive LOV2 domain allows anti-CRISPR activity...

11. How to Prepare for an Industry Interview

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    Blog Post

    ...PI of the lab and for one position, I just had a phone interview. I also transitioned between different...

12. Adenoviral Vector Production and Troubleshooting

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    Blog Post

    ...looking at absorbance at 260nm using UV/Vis spectrophotometry. An absorbance of at 260nm corresponds to...

13. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

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    ... Addgene account username. Shipping address and phone number. Plasmid sequences or GenBank files. We encourage...

14. Overcoming the Challenges of Lentiviral Production

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    ...transfection method (i.e. polyethyleneimine, calcium phosphate or liposomal methods), the volume of the reaction...

15. Popular Retroviral Vectors and Their Uses in Scientific Research

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    ...genes via infection with a single ecotropic or amphotropic MuLE lentivirus provides significant genetic ...

16. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

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    Blog Post

    ...change to large insertions like the addition of a fluorophore or tag. To utilize HDR, a donor template containing...

17. Plasmids 101: Modular Cloning

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    Blog Post

    ...Level 1 TUs with unique promoters, and the ideal phosphatase (or any modifying enzyme) to pair with it can...

18. 5 Tips for Troubleshooting Viral Transductions

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    Blog Post

    ...fibronectin-enhanced retroviral transduction of primary human T lymphocytes for adoptive immunotherapy." Acute Leukemias ...

19. Gaining Leadership Skills Volunteering at a Professional Organization

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    Blog Post

    ... with other people mostly online and on monthly phone calls. This has its challenges as meeting in person...

20. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

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    Blog Post

    ...discuss important issues in person or over the telephone instead of email. As you cannot see a person’s...