We narrowed to 404 results for: pho;

1. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

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   ...al disrupted interactions between Cas9 and the phophate backbone of the target DNA strand via mutations...

2. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

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   ...FPs optimized for oxidizing environments to photoconvertible variants, it seems like GFP is always learning...

3. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

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   ...Louisiana State University. She is a fan of photosynthesis and writes about autotrophs and biochemistry...

4. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

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   ...change to large insertions like the addition of a fluorophore or tag. To utilize HDR, a donor template containing...

5. Overcoming the Challenges of Lentiviral Production

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   ...transfection method (i.e. polyethyleneimine, calcium phosphate or liposomal methods), the volume of the reaction...

6. Popular Retroviral Vectors and Their Uses in Scientific Research

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   ...genes via infection with a single ecotropic or amphotropic MuLE lentivirus provides significant genetic ...

7. Adenoviral Vector Production and Troubleshooting

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   ...looking at absorbance at 260nm using UV/Vis spectrophotometry. An absorbance of at 260nm corresponds to...

8. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

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   ...CRISPR-Cas activity. For example, fusing AcrA4II to the photo-sensitive LOV2 domain allows anti-CRISPR activity...

9. How to Prepare for an Industry Interview

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   ...PI of the lab and for one position, I just had a phone interview. I also transitioned between different...

10. Plasmids 101: Modular Cloning

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    ...Level 1 TUs with unique promoters, and the ideal phosphatase (or any modifying enzyme) to pair with it can...

11. Hot Plasmids February 2024

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    ...Ando et al., 2023). This variant has similar photostability and brightness compared to the original, though...

12. CRISPR in the Clinic

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    ...CRISPR spin. CRISPR CAR-T treatments for leukemia, lymphoma, and some solid tumors are currently in early-...

13. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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    ...quantification of various intermediate steps, a sizing electrophoretic gel, and qPCR quantification of the final libraries...

14. Hot Plasmids and Viral Preps - March 2021

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    ....   G-baToN can also be applied to other fluorophore/antibody pairs. Because the anti-GFP nanobody...

15. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

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    ... Addgene account username. Shipping address and phone number. Plasmid sequences or GenBank files. We encourage...

16. 5 Tips for Troubleshooting Viral Transductions

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    ...fibronectin-enhanced retroviral transduction of primary human T lymphocytes for adoptive immunotherapy." Acute Leukemias ...

17. Gaining Leadership Skills Volunteering at a Professional Organization

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    ... with other people mostly online and on monthly phone calls. This has its challenges as meeting in person...

18. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

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    ...discuss important issues in person or over the telephone instead of email. As you cannot see a person’s...

19. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

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    ... of the highly conserved glutamine-fructose-6-phosphate aminotransferase (glmS) gene in the recipient ...

20. Fluorescent Tagging of Endogenous Genes with SapTrap

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    ...about Fluorescent Protein Timers Check out Photoactivatable Fluorescent Proteins Use Rosella to Monitor...