...inhibitor of uracil DNA glycosylase (UGI) to dCas9 prevents base excision repair, which would change the U...of inosine from DNA is likely infrequent, thus preventing the induction of base excision repair. In terms...improved base editing capacity, even at sites previously difficult to target. ABE8s can achieve 98-99%...

...water bath with distilled water, not tap water, to prevent salts from accumulating on the surfaces of the ...evaporates from the tub. Disinfectant may be added to prevent growth of bacteria or fungi. There are disinfectants...water bath cover on the top of the water bath to prevent evaporation and maintain the desired temperature...in the lab, you can leave the water in it from previous uses. However, you will still need to regularly...

...that enables high school students to publish peer-reviewed scientific research. You’ll meet some of the folks...mouth and personal networking!) and the manuscript review/submission process at JEI. Chapter 3 - JEI target...

...DeckTheLab and tagging us, @addgene to be judged! In previous years, scientists have decorated their labs with...on the entries, select up to three winners, and reveal the winning entries on Twitter and Instagram! Happy...

...microbiology lab every year for over 20 years. Previously, the lab used three different species for the...continues until all quadrants are used, hopefully revealing single, isolated colonies in the last quadrant...

... expression of specific genes in the brain in reversible fashion. And that’s exactly what the LITEs now...response can be graded with light dose and is fully reversible after light retraction. Finally, because light...

...ITR: ITR Forward Primer: 5’-CGGCCTCAGTGAGCGA ITR Reverse Primer: 5’-GGAACCCCTAGTGATGGAGTT ITR Probe: -FAM-CACTCCCTCTCTGCGCGCTCG-BBQ...Forward ITR Primer (10 µM) 1.8 µL 16.2 µL 900 nM Reverse ITR Primer (10 µM) 1.8 µL 16.2 µL 900 nM Nuclease-free...tube, gently uncap the tubes. Add 5 µL of the previously aliquoted 1X dilution buffer from step 9 to the...titering using ddPCR, read our blog post. Last reviewed on: August 31, 2023...

... affects the solubility of the RNA. Pro-Tip To prevent overdrying, watch the pellet and carefully remove...for RNA. Store your RNA sample(s) at -80 °C to prevent RNA degradation and avoid multiple freeze-thaw ... for RNA.Store your RNA sample(s) at -80 °C to prevent RNA degradation and avoid multiple freeze-thaw ...

...identify mutations of unknown importance in cancer Previously available methods for investigating and functionally...that that confer proliferation advantages will be revealed in the absence of growth factor. The team assigned...

...honest feedback DePace’s yearly planning meetings revolve around two simple worksheets, each of which is ...sections, the trainee speaks first for each section, preventing the meeting from feeling like an assessment or...

... stage is often much easier said than done. As reviewed in our Plasmids 101 series, proteins are overexpressed...protein expression and purification: a comprehensive review of affinity tags and microbial applications." Biotechnology...

...x-ray machine not on teeth, but on blossoms, thus revealing the startling inner beauty of familiar flowers...such as those collected by Darwin on his voyage. Previous destructive techniques were incompatible with ...

...another is not just to share your riches but to reveal to him his own." -Benjamin Disraeli Mentor, Sponsor...and more. It is instructive (and entertaining) to review this list of bad mentor behaviors. Sadly, it is...

...repression of gene expression in Saccharomyces cerevisiae. The system consists of dCas9, the mammalian ...multiplexed gRNAs in human pluripotent stem cells. Previously, CRISPRi or CRISPRa depended on continued expression...

...assembly from cryo-electron microscopy, the team reveal the mechanism behind its activity including an ...motif, averaging 19 targets per gene. The lab has previously used the library with SpdCas9 to perform pooled...

...As previous blogs have noted, plants are an important foundation for life on Earth. Selective breeding... resistance. Much of plant genetic engineering revolves around Agrobacterium tumifaciens. Agrobacterium...

...transcriptional activation/repression. dCas9 has also previously been adapted to fluorescently label a sequence...calculated fluorescent distances correlating with the previously established physical map. In comparing pairs ...

...Cre-estrogen receptor ligand binding domain-fusion can prevent this toxicity, but with the caveat of partial rather...toxicity. FLP/FRT recombinases (from Saccharomyces cerevisiae) had been used successfully in Drosophila, so...

...to control their own future in science (see my previous blog on this).  Indeed, that was a primary message...rules and practices of indirect costs.  The peer review and grant awards systems need to be radically overhauled...

...variety of careers outside of academia. She has had previous graduate students and postdocs go on to work in...what you are truly passionate about. Check out my previous post if you're looking for more information about...