...journal’s submission rules for review articles Whether submitting a review by invitation or by your own ...discussing the review topic with your peers. Start reading! I started by reading other reviews because, as...Writing a review article is a wonderful way to develop and exercise your scientist skill set. If you ...you dread the thought of writing a review, or if you’re currently stuck trying to write one, hopefully this...field and are the perfect person to be writing the review! Doing so is a great way to develop your ability...information: basically, a scientist’s tool kit. I wrote a review article on histone modifers in B and T cells with...thing I did was google “how to write a scientific review.” The second thing I did was stare in horror at...

...co-founders of Revolution Bioengineering.  Last year we started a company. Revolution Bioengineering ... how feasible they were, conducting literature reviews and having weekly journal clubs outside of work...process, we had an idea that survived the literature review, survived technical feasibility analysis, and was...accomplish the color change until a few months later. RevBio accelerates with help from SynBio Axlr8r We limped... revbio...

..., etc. Also, a constant in-flow of individuals wherever the cell cultures are kept will increase the risk...detection of mycoplasmas difficult (Ligasová, 2019). Prevention of mycoplasma contamination The best and the ...humans are the major source of contamination, the prevention steps start from you. If you are careful enough...contamination but it’s a very good practice for prevention. Prepare a small quantity of media stock that...that would be used for the experiment, preventing contamination of the rest. Avoid long exposure of the ...only be used to remove mycoplasma and not for preventing mycoplasma growth. Also, prolonged use of a particular...Conclusion To sum up, it is always important to prevent contamination rather than eradicating it later....

...ori) which allow it to replicate.   How can I prevent recombination in my samples? Plasmid recombination...Huang C, Liang Z, Ma X, Wang N, Huo Y-X (2020) Reversed paired-gRNA plasmid cloning strategy for efficient...

...pyogenes that locks itself together, to produce irreversible protein-peptide interactions. In a study published... peptide tags, which often form only weak and reversible bonds. Howarth’s team has already demonstrated...SpyCatcher system, to be able to grab on to proteins irreversibly in a genetically encoded way. SpyTag is nice...

...expression vectors. And more broadly, their data revealed that the longer a natural gene sequence is, the...

...early peer-review attempts took place at the beginning of the 18th century. Yet the peer-review was not ...the DNA, were not peer-reviewed (Baldwin, 2015). At its very core, peer-review is a touchstone of the...peer-reviewer will help spot inconsistencies in the work, missing information that would prevent the study... easier for me to go back and finish the review.  Peer-review step 2: Detailed assessment After this initial...the peer-reviewer you wish for The last thing that I wanted to mention about the peer-review, is that ... in reviewing the paper of your colleagues. The young scientists that are reading your peer-review comments...perspective of an author submitting a paper, the peer-review seems like another dragon to slay on the way to...

... pRSV-Rev - This 3rd generation lentiviral packaging plasmid from the Trono lab contains the rev gene....Didier Trono lab that expresses the HIV gag, pol, rev, and tet genes. It can be used with 2nd or 3rd generation...pMD2.G, both deposited from the same lab. Find pRSV-Rev. pMDLg/pRRE - This is a 3rd generation lentiviral...contains the gag and pol genes. It also requires pRSV-Rev and the envelope expressing plasmid pMD2.G. Find ...contains all the necessary elements for packaging, reverse transcription, and integration needed for the production...

...plasmid for expressing a novel reverse transcriptase (RT), MarathonRT. Reverse transcriptases are critical...Plasmids Podcast!  MarathonRT: A highly processive reverse transcriptase Article contributed by Will Arnold...outperforms other RTs in this regard and is even able to reverse transcribe the extremely long, highly structured...techniques that are dependent on long, error-free, reverse transcription of RNA such as long-read cDNA sequencing...

...packaging plasmid encoding HIV proteins gag, pol, rev, and tat. 3rd generation systems require an envelope..., one encoding gag and pol and a second encoding rev.  Secondly, 3rd generation packaging systems have.... Third-generation vectors contain gag, pol, and rev. Second-generation contains those three genes plus...specific tissues are areas of active study (for a review click here). Alternative envelopes are powerful...the 3'LTR in the transfer plasmid. The deletion prevents full-length viral RNA from being produced in target...

... HIV Tat gene (previously used to drive expression from the LTRs) has been removed, Rev (which facilitates...the production of a viral particle (Gag, Pol, Tat, Rev and Env) and the transfer plasmid encoding the insert... sequence, a part of the env gene containing the rev response element (RRE), an internal promoter, and...vector requires only three HIV-1 genes (gag, pol, and rev) for production. Third generation lentiviral vectors...cells transduced by a GFP encoded virus, and the reverse transcriptase activity assay uses qPCR to quantify... All of these methods have been described in a previous Addgene blog Once you know your viral titre, you...and U. Stein. "Viral vectors for gene transfer a review of their use in the treatment of human disease....

...sulfur amino acids in Saccharomyces cerevisiae. Microbiol Mol Biol Rev 61, 503-532. (1997). PubMed PMID:...10436161. 7. Dujon, B. Yeast evolutionary genomics. Nat Rev Genet 11, 512-524. (2010). PubMed PMID: 20559329....the S. cerevisiae lineage (7). In our class research project, students transform S. cerevisiae met deletion...synthesis (5). The budding yeast, Saccharomyces. cerevisiae, makes a great reference organism for the project... is available (6). Methionine synthesis in S. cerevisiae (Figure 1) occurs through a well-characterized...the same function as their counterparts in S. cerevisiae. The two yeasts are thought to have diverged ...carrying MET/Met genes from either S. pombe or S. cerevisiae. Students then determine if expression of the...

... transcriptional activation from the 5’ LTR) and Rev (facilitates nuclear export of transcripts). A different... system is split into two plasmids: one encoding Rev and one encoding Gag and Pol, increasing the number... vectors contain a deletion in the 3’ LTR that prevents aberrant activation of nearby genes. These safer...

... human induced pluripotent stem cells. Stem Cell Rev, 2015. 11(2): p. 242-53. PubMed PMID: 25578634. PubMed...reprogramming: three routes to regeneration. Nat Rev Mol Cell Biol, 2011. 12(2): p. 79-89. PubMed PMID... and transdifferentiation are back in style. Nat Rev Mol Cell Biol, 2016. 17(7): p. 413-25. PubMed PMID...via: ReprogrammingA differentiated somatic cell reverts back to a pluripotent state, which then proliferates...pluripotent stage. Cellular reprogramming The reversal of a differentiated cell type to an undifferentiated... - that induce a differentiated somatic cell to revert back to a pluripotent stage (iPSC formation) [2...Trends in Pharmacological Sciences, a Cell Press reviews journal. She joined Cell Press to pursue a career...

...for CRISPR? A user’s guide to genetic screens. Nat Rev Genet 19:67–80 . https://doi.org/10.1038/nrg.2017.97...the genome and transcriptome of living cells. Nat Rev Genet 19:770–788 . https://doi.org/10.1038/s41576...discussion of these subjects can be found in two recent review articles (Doench et al., 2017, and Hanna et al.... single cell cloning – as a control, one should revert the edit back to the original in order to prove...the window. Another technology, prime editing (reviewed in Anzalone et al., 2020). is not limited to single...

...Deisseroth New serotype (multiple) rAAV-CAG::FLEX-rev:: hM4D-2a-GFP AAV1 Chemogenetics Scott Sternson New...

... CRISPR-Cas in drug development and therapy. Nat Rev Drug Discov (2017) 16(2):89-100. DOI: 10.1038/nrd...they are deeply intertwined. In this blog we will review how CRISPR technology has brought drug and genetic...affect disease progression or onset. These hits can reveal individual genes or sometimes even genetic pathways...proficient and deficient for the target. CRISPR has revolutionized the generation of knockout cell lines which...

...modify clinical-grade T cells to target CD19. Immunol Rev. 2014 Jan;257(1):181-90. doi: 10.1111/imr.12137. ...

...References Robinson HL. Retroviruses and cancer. Rev Infect Dis. 1982 Sep-Oct;4(5):1015-25. DOI: 10.1093...Retroviruses are single-stranded RNA viruses that use reverse transcriptase to synthesize complementary DNA copies...their features Choosing a virus Now that we’ve reviewed the common viruses used in biological research...