...Generation Type Description PI 12253 pRSV-Rev 3rd Packaging Contains Rev; can be used with 3rd generation transfer...with a plasmid encoding Rev Trono 12260 psPAX2 2nd Packaging Contains Gag, Pol, Rev, and Tat; can be used...pCMV delta R8.2 2nd Packaging Contains Gag, Pol, Rev, and Tat; can be used with 2nd and 3rd generation...pCMV-dR8.2 dvpr 2nd Packaging Contains Gag, Pol, Rev, Tat, Vif, and Nef; can be used with 2nd and 3rd ...22500 pNHP 2nd Packaging Contains gag, pol, tat, rev Kasparov 1732 pCPRDEnv 2nd Packaging part of the ...the FELIX vector system, expresses Gag-Pol and Rev Nolan 35617 pCAG-Eco N/A Envelope Ecotropic MLV expressing...pCD/NL-BH*DDD 2nd Packaging Contains Gag/Pol, Tat, Rev. Use with envelope plasmids from Jakob Reiser. Reiser...

...suppression. Bieging KT, Mello SS, Attardi LD. Nat Rev Cancer. 2014 May;14(5):359-70. PubMed PMID: 24739573...from the mutant p53 field. Brosh R, Rotter V. Nat Rev Cancer. 2009 Oct;9(10):701-13. PubMed PMID: 19693097...p53 targets. Menendez D, Inga A, Resnick MA. Nat Rev Cancer. 2009 Oct;9(10):724-37. PubMed PMID: 19776742...

...AAV9 AAV-FLEX-rev-ChR2(H134R)-mCherry Scott Sternson AV-9-18917P 18917-AAV9 AAV-FLEX-rev-ChR2-tdtomato... James M. Wilson AV-1-18917P 18917-AAV1 AAV-FLEX-rev-ChR2-tdtomato Optogenetics Scott Sternson AV-1-20071P...Addgene ID Name PI AV-1-18916P 18916-AAV1 AAV-FLEX-rev-ChR2(H134R)-mCherry Scott Sternson AV-1-20949P 20949...GENIE, Douglas Kim AV-10-18917P 18917-AAV1 AAV-FLEX-rev-ChR2-tdtomato Scott Sternson AV-10-PV1963 105542-... James M. Wilson AV-5-18917P 18917-AAV5 AAV-FLEX-rev-ChR2-tdtomato Scott Sternson AV-5-20071P 20071-AAV5...

... L, Sonenberg N, Pelletier J, Topisirovic I. Nat Rev Drug Discov. 2015 Apr;14(4):261-78. doi: 10.1038/...

...none 1, 2, 5, 8, 9, rg* Roth 52536 rAAV-CAG::FLEX-rev:: hM4D-2a-GFP hM4D(Gi) - Inhibition GFP Cre-dependent...

...probes targeting integrated copies of the lentiviral Rev responsive element (RRE) gene and the cellular ribonuclease... et al. (2014) (Link opens in a new window) ). Previous experience suggests that titers obtained using...

...signalling pathways in cancer therapy. Downward J. Nat Rev Cancer. 2003 Jan;3(1):11-22. PubMed PMID: 12509763...

...tetracycline resistance. Hillen W and Berens C. Annu Rev Microbiol . 1994;48:345-69. PubMed . Transcriptional...led to the development of a reverse Tet repressor, or rTetR, which reversed the phenotype and created a...26429 pLenti CMV rtTA3 Blast (w756-1) Lentiviral reverse tetracycline-controlled transactivator 3 (rtTA3...

...biological progress over 50 years in Rett syndrome. Nat Rev Neurol . 13, 37–51. PMID: 27934853 Neul et al. 2008...RNA trans-splicing, and protein replacement to reverse the impacts of this disease. Addgene is working...typically between 18 to 30 months, is regression of previously acquired skills, notably loss of acquired purposeful... demonstrates that Rett syndrome symptoms are reversible in mice if MECP2 gene expression is switched ... a new window) PMID: 24399845 Guy et al. 2007. Reversal of Neurological Defects in a Mouse Model of Rett...Laurvick et al. 2006. Rett syndrome in Australia: a review of the epidemiology. J Pediatric . 148(3):347-352...PMID: 18337588 Neul et al. 2010. Rett syndrome: revised diagnostic criteria and nomenclature. Ann Neurol...

...journal’s submission rules for review articles Whether submitting a review by invitation or by your own ...discussing the review topic with your peers. Start reading! I started by reading other reviews because, as...Writing a review article is a wonderful way to develop and exercise your scientist skill set. If you ...you dread the thought of writing a review, or if you’re currently stuck trying to write one, hopefully this...field and are the perfect person to be writing the review! Doing so is a great way to develop your ability...information: basically, a scientist’s tool kit. I wrote a review article on histone modifers in B and T cells with...thing I did was google “how to write a scientific review.” The second thing I did was stare in horror at...

...co-founders of Revolution Bioengineering.  Last year we started a company. Revolution Bioengineering ... how feasible they were, conducting literature reviews and having weekly journal clubs outside of work...process, we had an idea that survived the literature review, survived technical feasibility analysis, and was...accomplish the color change until a few months later. RevBio accelerates with help from SynBio Axlr8r We limped... revbio...

..., etc. Also, a constant in-flow of individuals wherever the cell cultures are kept will increase the risk...detection of mycoplasmas difficult (Ligasová, 2019). Prevention of mycoplasma contamination The best and the ...humans are the major source of contamination, the prevention steps start from you. If you are careful enough...contamination but it’s a very good practice for prevention. Prepare a small quantity of media stock that...that would be used for the experiment, preventing contamination of the rest. Avoid long exposure of the ...only be used to remove mycoplasma and not for preventing mycoplasma growth. Also, prolonged use of a particular...Conclusion To sum up, it is always important to prevent contamination rather than eradicating it later....

...ori) which allow it to replicate.   How can I prevent recombination in my samples? Plasmid recombination...Huang C, Liang Z, Ma X, Wang N, Huo Y-X (2020) Reversed paired-gRNA plasmid cloning strategy for efficient...

...pyogenes that locks itself together, to produce irreversible protein-peptide interactions. In a study published... peptide tags, which often form only weak and reversible bonds. Howarth’s team has already demonstrated...SpyCatcher system, to be able to grab on to proteins irreversibly in a genetically encoded way. SpyTag is nice...

...expression vectors. And more broadly, their data revealed that the longer a natural gene sequence is, the...

...early peer-review attempts took place at the beginning of the 18th century. Yet the peer-review was not ...the DNA, were not peer-reviewed (Baldwin, 2015). At its very core, peer-review is a touchstone of the...peer-reviewer will help spot inconsistencies in the work, missing information that would prevent the study... easier for me to go back and finish the review.  Peer-review step 2: Detailed assessment After this initial...the peer-reviewer you wish for The last thing that I wanted to mention about the peer-review, is that ... in reviewing the paper of your colleagues. The young scientists that are reading your peer-review comments...perspective of an author submitting a paper, the peer-review seems like another dragon to slay on the way to...

... pRSV-Rev - This 3rd generation lentiviral packaging plasmid from the Trono lab contains the rev gene....Didier Trono lab that expresses the HIV gag, pol, rev, and tet genes. It can be used with 2nd or 3rd generation...pMD2.G, both deposited from the same lab. Find pRSV-Rev. pMDLg/pRRE - This is a 3rd generation lentiviral...contains the gag and pol genes. It also requires pRSV-Rev and the envelope expressing plasmid pMD2.G. Find ...contains all the necessary elements for packaging, reverse transcription, and integration needed for the production...

...plasmid for expressing a novel reverse transcriptase (RT), MarathonRT. Reverse transcriptases are critical...Plasmids Podcast!  MarathonRT: A highly processive reverse transcriptase Article contributed by Will Arnold...outperforms other RTs in this regard and is even able to reverse transcribe the extremely long, highly structured...techniques that are dependent on long, error-free, reverse transcription of RNA such as long-read cDNA sequencing...

...evolutionary classification of CRISPR-Cas systems. Nat Rev Microbiol . 13(11):722-36. PMID: 26411297 Mali P,...gRNA Design Tools CRISPR Blog Posts The CRISPR revolution shows no signs of slowing down. This prokaryotic...employ varied mechanisms to block CRISPR. Some prevent the CRISPR-Cas complex from binding to DNA. Others...or repressors can modulate transcription in a reversible manner. Epigenetics : dCas9 fused to epigenetic...Plants (monocots and dicots) C. elegans Yeast ( S. cerevisiae and S. pombe ) Zebrafish Xenopus References Barrangou...