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We narrowed to 601 results for: sam

Showing: 301 - 320 of 601 results
  1. Career Insights: Technical Support Specialist

    Type
    Blog Post
    ...Support is essential for success. For possibly this same reason, it can be one of the more constant and safe...many different backgrounds and realizing that the same approach does not work for everyone. The benefit...
  2. New Tools Enable CRISPRa for Neuroscience Applications

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    Blog Post
    ... Gene isoforms refer to mRNAs generated from the same gene but with different untranslated or coding regions...transcriptional start sites, all of which yield the same BDNF protein. To demonstrate the specificity that...
  3. Neuronal labeling with Spaghetti Monster

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    Blog Post
    ...super-resolution STORM imaging, and electron microscopy. Sample preparation for these techniques sometimes makes...smFPs retain their ability to be detected after sample preparation better than standard proteins tags ...
  4. Hot Plasmids Spring 2024

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    Blog Post
    ...#211756 (left panel) or #211757 (middle panel). Samples were also immunoblotted against vinculin as a loading...during action potentials of fast-spiking neurons. The same A-to-D mutation in the related voltage indicator...
  5. To Codon Optimize or Not: That is the Question

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    Blog Post
    ...amino acids and three stop codons, meaning that the same amino acid can be encoded by more than one codon...organisms. There are several codons that encode the same amino acid. Each organism, however, may preferentially...
  6. The Challenges of Cell Culture

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    Blog Post
    ...materials available from commercial repositories and the same cell line from a colleague?”. The main difference...) profiling can be used to ID the cell line. The same test is so good that it is used as a forensic technique...
  7. Getting the Most from Your Lentiviral Transduction

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    Blog Post
    .... Know your target cells Labs tend to follow the same general procedure for lentiviral infections, produce...but also between different lots of serum from the same source. Labs that routinely produce lentivirus may...
  8. Tips for Using FRET in Your Experiments

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    Blog Post
    ...What is FRET? In their JCB 2003 paper, Sekar and Periasamy defined FRET as "a distance-dependent physical...341-50. doi: 10.1002/bies.201100098. Sekar RB, Periasamy A. Fluorescence resonance energy transfer (FRET...
  9. CRISPR 101: Validating Your Genome Edit

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    Blog Post
    ... as a proxy for your edit of interest. Using the same gRNA and donor, you can deliver a silent mutation...to compare the sequencing reads from your edited sample to an untreated population. Software such as CRISPResso...
  10. An Integrin Antibody Toolkit from IPI

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    Blog Post
    ...Cells often express multiple integrins that bind the same ligand, making it difficult to dissect the function...extracellular matrix. During immune responses, this same conformation encourages immune cell adhesion and...
  11. The Fluorescent Vegetables in Aptamer Soup

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    Blog Post
    ...orange fluorescent aptamers Corn was described in the same study as Red Broccoli and Orange Broccoli, two more...PMC5679246. Strack, Rita L., Matthew D. Disney, and Samie R. Jaffrey. "A superfolding Spinach2 reveals the...
  12. Fluorescent Proteins 101: When GFP lets you down

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    Blog Post
    ... proteins are also a better choice for thicker samples. Another solution to avoid autofluorescence is ...reaction and hence, there is no need to excite the sample. Although bioluminescent probes are usually dim...
  13. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

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    Blog Post
    ...activate or repress target genes in plants using the same general experimental design principles developed... expression of both Cas9 and the gRNA off of the same plasmid. Delivering CRISPR components to plant cells...
  14. Viral Vectors 101: AAV Serotypes and Tissue Tropism

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    Blog Post
    ...doi.org/10.1074/jbc.M101559200 Wu, Z., Asokan, A., & Samulski, R. J. (2006). Adeno-associated virus serotypes....2006.05.009 Wu, Z., Miller, E., Agbandje-McKenna, M., & Samulski, R. J. (2006). Α2,3 and α2,6 N-Linked Sialic Acids...
  15. Plasmids 101: Repressible Promoters

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    Blog Post
    ...system to GAL4/UAS that functions in essentially the same manner, with LexA binding to the lexA operator (...desirable to control or look at multiple genes at the same time.  The 3 binary systems listed above are orthogonal...
  16. Antibodies 101: Antibody Engineering and Directed Evolution

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    Blog Post
    ... about these methods is that they all follow the same general cyclical process (Figure 2). The differences...codons (different codon sequences that code for the same amino acid) for various amino acids. Essentially...
Showing: 301 - 320 of 601 results