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We narrowed to 610 results for: sam

Showing: 301 - 320 of 610 results

  3. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...libraries of bait and prey against each other at the same time in order to try and access the entire interactome...the protein partner or the UAS/reporter gene, the same bait and prey libraries can be screened using both...

  4. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...often: Q1: Are retroviruses and lentiviruses the same thing? Can I package lentivirus with retroviral ...both lentiviruses and gamma-retroviruses use the same genes for packaging (that's gag, pol, and env), ...

  6. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ...different from other plasmids? If we all use the same molecular code, can I transfect any old bacterial...it’s pretty incredible that my cells “speak” the same molecular language as the tree outside my window...

  7. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...machinery in order to replicate—but not always to the same extent. As described in our previous Origin of Replication...proteins, it can be regulated indirectly via the same mechanisms that affect DnaA and IHF. RepA also controls...

  8. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...#211756 (left panel) or #211757 (middle panel). Samples were also immunoblotted against vinculin as a loading...during action potentials of fast-spiking neurons. The same A-to-D mutation in the related voltage indicator...

  9. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ...plasmids, all of which need to be represented in the same proportion after amplification as they were prior...visualization) that confirm diversity of the library sample, and any other information or scripts provided ...

  10. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ...possible to set up the BP and LR reactions in the same tube, speeding up the cloning of the attB-PCR products...multiple DNA fragments into many vectors at once in the same tube. You can clone up to 4 DNA fragments, in a ...

  11. Career Insights: Technical Support Specialist

    Type
    Blog Post
    ...Support is essential for success. For possibly this same reason, it can be one of the more constant and safe...many different backgrounds and realizing that the same approach does not work for everyone. The benefit...

  12. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ... Gene isoforms refer to mRNAs generated from the same gene but with different untranslated or coding regions...transcriptional start sites, all of which yield the same BDNF protein. To demonstrate the specificity that...

  14. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...super-resolution STORM imaging, and electron microscopy. Sample preparation for these techniques sometimes makes...smFPs retain their ability to be detected after sample preparation better than standard proteins tags ...

  15. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...amino acids and three stop codons, meaning that the same amino acid can be encoded by more than one codon...organisms. There are several codons that encode the same amino acid. Each organism, however, may preferentially...

  16. The Challenges of Cell Culture

    Type
    Blog Post
    ...materials available from commercial repositories and the same cell line from a colleague?”. The main difference...) profiling can be used to ID the cell line. The same test is so good that it is used as a forensic technique...

  17. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    .... Know your target cells Labs tend to follow the same general procedure for lentiviral infections, produce...but also between different lots of serum from the same source. Labs that routinely produce lentivirus may...

  18. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...What is FRET? In their JCB 2003 paper, Sekar and Periasamy defined FRET as "a distance-dependent physical...341-50. doi: 10.1002/bies.201100098. Sekar RB, Periasamy A. Fluorescence resonance energy transfer (FRET...

  19. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ... as a proxy for your edit of interest. Using the same gRNA and donor, you can deliver a silent mutation...to compare the sequencing reads from your edited sample to an untreated population. Software such as CRISPResso...
Showing: 301 - 320 of 610 results