We narrowed to 362 results for: tac

1. The Fluorescent Vegetables in Aptamer Soup

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   Blog Post

   ...with DFHBI, but its structure allows for greater contact with its RNA aptamer. This increased interaction...

2. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

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   ...that contains a fluorophore that is quenched when intact and released when cleaved. Cleavage results in ...

3. Transferable Skills Guide: Conflict Resolution

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   ...the past, or how completely different teams are tackling comparable conflicts. Is this level of domain ...

4. Tips for Getting a Faculty Position

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   Blog Post

   ...your first grant, how you will overcome certain obstacles, alternative approaches, potential collaborations...

5. Antibodies 101: Beyond Surface Labeling

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   ...more. This process, however, leaves the nucleus intact. If markers of interest are located inside the ...

6. An Integrin Antibody Toolkit from IPI

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   ...ligand-binding pocket, their sheer size impeding ligand attachment. The five other IPI rAbs bind RGD-binding integrins...

7. CRISPR 101: Validating Your Genome Edit

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   ...can ensure you aren’t looking for a needle in a haystack before going on to generate and screen individual...

8. Filming Science Videos in the Age of Social Distancing

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   ...mounted a monitor to it. It had an extension cord attached, with the option to mount a battery to it if necessary...

9. Multiple Plasmids at a Low Price: Kits from Addgene

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   ...research. If you’d ever like to deposit a kit, please contact us at [email protected]. ...

10. 10 Steps to a Perfect Science Talk

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    Blog Post

    ...Describe how you pushed to a solution or overcame obstacles. Take time to consider the order of your narrative...

11. How to Negotiate a Successful Lab Start Up Budget

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    ...your lab. That in itself is a strong negotiating tactic, and puts you ahead of most start-up P.I.’s. It...

12. Technologies Enabled by NanoLuc® Luciferase

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    ...around the world and had specific strategies and tactics for getting the plasmids he wanted. Addgene makes...

13. How to Design Your gRNA for CRISPR Genome Editing

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    ...clonal heterogeneity may represent a more serious obstacle to the generation of truly isogenic cell lines...

14. Recombinase-based State Machines Enable Order-dependent Logic in vivo

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    ... perturbation. Also, this method requires live, intact cells; the sequencing and qPCR methods do not. ...

15. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

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    ...chicory root, but considering this is a needle in a haystack type of objective, it was surprisingly easy. It...

16. A History of Genome Engineering in Popular Culture

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    ...wreaking havoc in a theme park.  Released in 1997, GATTACA is an American SciFi film starring Ethan Hawke,...

17. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

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    ...recombination rate of the vector you are using. Contact Addgene ([email protected]), or the supplier of ...

18. Live and Let Dye: Self-Labeling Protein Tags

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    ... strategies include using engineered ligases to attach a ligand to a peptide tag on your protein of interest...

19. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

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    Blog Post

    ... if you have any questions, do not hesitate to contact me at [email protected]. Keep pipetting and...

20. Custom CRISPR Screens & the Green Listed Software

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    ... to target surface proteins to determine which contact points are involved in a studied cellular behavior...