... To generate a specific sequence change the cell must undergo homology-directed repair (HDR), a distinct...the cells. However, our excitement turned into frustration when we sequenced our cells and found how inefficiently...deep sequencing, but interestingly (and to our frustration) in many cases we only saw incorporation of the...but not the other – the efficiency of editing is just too low for one to expect two successful mutations...

... al is a PCR-based approach for generating a customizable comprehensive mutagenesis library that’s ready...plasmid. If the sites are in opposite directions, you just need to use a plasmid with BbvCI restriction sites...sites in the same orientation as the gene or use custom gene synthesis or site directed mutagenesis to .... PubMed PMID: 20676978. 3. Wrenbeck, Emily E., Justin R. Klesmith, James A. Stapleton, Adebola Adeniran...

...loop do not fully activate MEK1. In fact, replacing just one of the MEK1 phosphomimetic residues in the above...can also be cloned with an ORF of interest for customized experiments. Once the ORF of interest is cloned...the amino acid to be replaced with phosphoserine must be recoded to ‘TGA’ using site-directed mutagenesis...specifically to phosphate moieties and promotes a robust mobility shift in phosphorylated, but not non-phosphorylated...

...first time live tweeting: a fun and surprisingly exhausting experience. As Addgene believes many of the topics...note, it was highlighted during the conference that just increasing funding for academic research will not...sought to make an interactive game that highlights just this point. As part of this, trainees need to be... impending problems. This can lead to a lack of trust in the mentor and this training is clearly not equitable...

... sciences and featured speakers from academia, industry, nonprofits, and publishing. The livestream video... the panel, irreproducibility undermines public trust in science and research. Nonetheless, I’d like to... the health of the scientific enterprise and not just impact. Cause #3: Poor experimental design It’s ...publishing community views reproducibility, how industry relies on reproducibility, and how you can push...

...? Or maybe you just want to make some really beautiful images? Multiplex IF may be just what you need!...different colors in one session – fast and easy! Just make sure your primary antibodies are all of different...the sample is not being degraded. Whether you’re just getting started with two or three targets or you...

... from drug treatment versus and which are likely just natural differences between individuals. Recommended...Antibody-based assays have their own unique controls that must be considered (Burry 2011). Perhaps the most critical...staining approaches, secondary antibody specificity must also be demonstrated. Secondary antibodies have ...antibody-based experiments, it is by no means an exhaustive list and we encourage you to review the literature...

..., it could lead to greater off-target expression just due to more overall viral particles getting into...to begin with. Now you will mainly only have to adjust the titer in order to minimize off-target expression...interest. You should now be able to make smaller adjustments to a set of injections into your animal model...optimizing all conditions first, you will save time, frustration, and funds in the long-run by ensuring you are...

...Gregor Mendel on the genetic inheritance (1866) and just when the Mendelian genetics was being revived by... Park by Michael Crichton was published in 1990, just a year after the discovery of meganucleases. The... a single-guide RNA sequence.  The Cas proteins, just like its predecessor induce a double-strand break... potentially kill one-third of the human beings (just like the black plague).  Base editors The major ...

...advertise for Group Leader positions starting in August, the year before the position would begin- for ...position yourself for a job search. Therefore, you must balance these competing goals. Knowing when to follow...project are fundamental skills that all group leaders must master. While the calculus of these decisions is... a group leader. That said, being a PF can be exhausting. Now is the time to develop strategies to rest...

...this receptor to evade immune detection. We've only just touched the surface of immunotherapies currently...most efficient outcomes, collaborative therapies must be explored. When attacking cancer, multiple immunotherapy...efficiency. The objective of efficient immunotherapy must be to reach a flexible personalized treatment, and... If it is to be successful, cancer immunotherapy must aspire to be a field of healthcare as dynamic as...

...Feeling any better yet? If not, just remember that the next holiday break is just a few weeks away. Is your ...people to take some time out every once in a while – just read Melina Fan’s post on lunch. Why should our ...

...created a robust support system for scientists using materials in our repository, from our customer and science...scientists in 8,900 institutions across 111 countries. Just eight days into 2024, we've already shipped 2,078...

...renewable electricity is certainly not the only sustainability effort we’ve implemented at Addgene. The kitchen...check out Leila Haery’s blog series, “Bringing Sustainable Practices to the Lab.”  Whether participating...blog Subscribe to the Addgene blog Read our sustainability in the lab blog posts Read another blog post...

...challenge to the scientific research ecosystem, not just in the United States but around the world. As an...research institutions can turn to Addgene as a trusted partner for archiving their biological materials... seeking and expanding our collaborations with industry and public and private partners to create additional...

...anticipation. Their trust in the quality of the content is implicitly defined by their trust in the piñata ... tools published and documented by OMICtools. It must be confusing for a newcomer to decide on the right...development since its inaugural year in 2012. That’s just 3 to 4 years ago. It is a bubble of innovation (...

...LinkedIn for repository news and updates. Scientists must do science to be happy. What do we have to change...problem and for finding solutions. Even better than just reading this article - you should join Dr. Bourne... some organizations are woefully behind and some just don't have the resources to do enough. There are...

...buoyancy devices” (also known as gas vesicles) as acoustic biomolecule-based dyes. This technology is widely...about ‘Using ultrasound to image bacteria in vivo: acoustic reporter genes’ on our blog. Creative approaches.... References 1. Bourdeau, Raymond W., et al. "Acoustic reporter genes for noninvasive imaging of microorganisms...

...lab that has multiple collaborations, even with Industry labs Work on a big project that relies on a division...inviting others (be on a speaker committee) Follow industry publications like FierceBiotech to “learn the .... For More Reading: From Academic Solos to Industrial Symphonies by Gwen Acton, Alicia Gómez-Yafal ...

... assemble a large number of building blocks with just two or three inexpensive enzymes. Now, a new toolkit...traced the problem back to the endonuclease. “It’s just a sensitive protein,” he says. “There is nothing...do about it but be gentle. If it no longer works, just trash it.” Lohmann and his colleagues including ...